Restriction map for
pF1KE0596
(Linear) (Six-base) MAPSORT of: KIEE0596.seq Check: 6138 from: 1 to: 468
KIEE0596.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AleI CACnn'nnGTG
Cuts at: 0 391 468
Size: 391 77
AlwNI CAG_nnn'CTG
Cuts at: 0 429 468
Size: 429 39
ApaI G_GGCC'C
Cuts at: 0 70 365 468
Size: 70 295 103
Fragments arranged by size:
295 103 70
BanI G'GyrC_C
Cuts at: 0 148 298 468
Size: 148 150 170
Fragments arranged by size:
170 150 148
BanII G_rGCy'C
Cuts at: 0 28 70 183 354 365 468
Size: 28 42 113 171 11 103
Fragments arranged by size:
171 113 103 42 28 11
Bme1580I G_kGCm'C
Cuts at: 0 70 151 303 365 468
Size: 70 81 152 62 103
Fragments arranged by size:
152 103 81 70 62
BmgBI CAC'GTC
Cuts at: 0 393 468
Size: 393 75
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 468
Size: 152 316
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 268 468
Size: 268 200
BseYI C'CCAG_C
Cuts at: 0 21 51 62 144 308 434 468
Size: 21 30 11 82 164 126 34
Fragments arranged by size:
164 126 82 34 30 21 11
Bsp1286I G_dGCh'C
Cuts at: 0 28 70 151 183 303 354 365 468
Size: 28 42 81 32 120 51 11 103
Fragments arranged by size:
120 103 81 51 42 32 28 11
BsrFI r'CCGG_y
Cuts at: 0 280 371 468
Size: 280 91 97
Fragments arranged by size:
280 97 91
BstAPI GCAn_nnn'nTGC
Cuts at: 0 429 468
Size: 429 39
BtgI C'CryG_G
Cuts at: 0 78 89 221 294 354 468
Size: 78 11 132 73 60 114
Fragments arranged by size:
132 114 78 73 60 11
EaeI y'GGCC_r
Cuts at: 0 2 92 468
Size: 2 90 376
Fragments arranged by size:
376 90 2
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 152 468
Size: 152 316
EcoO109I rG'GnC_Cy
Cuts at: 0 66 67 137 362 468
Size: 66 1 70 225 106
Fragments arranged by size:
225 106 70 66 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 282 468
Size: 282 186
HaeII r_GCGC'y
Cuts at: 0 325 468
Size: 325 143
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 94 126 468
Size: 94 32 342
Fragments arranged by size:
342 94 32
MslI CAynn'nnrTG
Cuts at: 0 391 468
Size: 391 77
MspA1I CmG'CkG
Cuts at: 0 51 201 287 419 426 468
Size: 51 150 86 132 7 42
Fragments arranged by size:
150 132 86 51 42 7
NaeI GCC'GGC
Cuts at: 0 282 373 468
Size: 282 91 95
Fragments arranged by size:
282 95 91
NgoMIV G'CCGG_C
Cuts at: 0 280 371 468
Size: 280 91 97
Fragments arranged by size:
280 97 91
PpuMI rG'GwC_Cy
Cuts at: 0 137 468
Size: 137 331
PspOMI G'GGCC_C
Cuts at: 0 66 361 468
Size: 66 295 107
Fragments arranged by size:
295 107 66
PstI C_TGCA'G
Cuts at: 0 50 338 468
Size: 50 288 130
Fragments arranged by size:
288 130 50
PvuII CAG'CTG
Cuts at: 0 51 201 287 468
Size: 51 150 86 181
Fragments arranged by size:
181 150 86 51
SfcI C'TryA_G
Cuts at: 0 46 334 468
Size: 46 288 134
Fragments arranged by size:
288 134 46
StuI AGG'CCT
Cuts at: 0 236 468
Size: 236 232
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 166 322 468
Size: 166 156 146
Fragments arranged by size:
166 156 146
Tth111I GACn'n_nGTC
Cuts at: 0 216 468
Size: 216 252
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 200 468
Size: 200 268
Enzymes that do cut:
AleI AlwNI ApaI BanI BanII Bme1580I BmgBI BpmI
BseRI BseYI Bsp1286I BsrFI BstAPI BtgI EaeI Eco57MI
EcoO109I FalI HaeII Hin4I MslI MspA1I NaeI NgoMIV
PpuMI PspOMI PstI PvuII SfcI StuI TaqII Tth111I
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrGI BssHII
BssSI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NcoI NdeI NheI NotI NruI NsiI
NspI PacI PasI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StyI SwaI TatI TstI XbaI XhoI
XmaI XmnI ZraI