Restriction map for
pF1KE0595
(Linear) (Six-base) MAPSORT of: KIEE0595.seq Check: 4085 from: 1 to: 510
KIEE0595.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 83 510
Size: 83 427
AvaI C'yCGr_G
Cuts at: 0 34 81 129 256 510
Size: 34 47 48 127 254
Fragments arranged by size:
254 127 48 47 34
BamHI G'GATC_C
Cuts at: 0 307 510
Size: 307 203
BanI G'GyrC_C
Cuts at: 0 443 465 510
Size: 443 22 45
Fragments arranged by size:
443 45 22
BanII G_rGCy'C
Cuts at: 0 182 510
Size: 182 328
BbeI G_GCGC'C
Cuts at: 0 469 510
Size: 469 41
BglII A'GATC_T
Cuts at: 0 416 510
Size: 416 94
BmgBI CAC'GTC
Cuts at: 0 265 510
Size: 265 245
BsaBI GATnn'nnATC
Cuts at: 0 415 510
Size: 415 95
BsaHI Gr'CG_yC
Cuts at: 0 466 510
Size: 466 44
BsaWI w'CCGG_w
Cuts at: 0 174 304 485 510
Size: 174 130 181 25
Fragments arranged by size:
181 174 130 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 510
Size: 316 194
BsmBI CGTCTCn'nnnn_
Cuts at: 0 255 382 510
Size: 255 127 128
Fragments arranged by size:
255 128 127
Bsp1286I G_dGCh'C
Cuts at: 0 182 510
Size: 182 328
BspEI T'CCGG_A
Cuts at: 0 174 510
Size: 174 336
BsrBI CCG'CTC
Cuts at: 0 117 510
Size: 117 393
BsrDI GCAATG_nn'
Cuts at: 0 134 359 498 510
Size: 134 225 139 12
Fragments arranged by size:
225 139 134 12
BsrFI r'CCGG_y
Cuts at: 0 233 510
Size: 233 277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 488 510
Size: 488 22
BstYI r'GATC_y
Cuts at: 0 307 416 510
Size: 307 109 94
Fragments arranged by size:
307 109 94
EaeI y'GGCC_r
Cuts at: 0 380 510
Size: 380 130
EarI CTCTTCn'nnn_
Cuts at: 0 143 368 429 510
Size: 143 225 61 81
Fragments arranged by size:
225 143 81 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 510
Size: 34 476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 366 510
Size: 366 144
HaeII r_GCGC'y
Cuts at: 0 469 510
Size: 469 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 69 101 510
Size: 69 32 409
Fragments arranged by size:
409 69 32
KasI G'GCGC_C
Cuts at: 0 465 510
Size: 465 45
MfeI C'AATT_G
Cuts at: 0 503 510
Size: 503 7
MscI TGG'CCA
Cuts at: 0 382 510
Size: 382 128
NarI GG'CG_CC
Cuts at: 0 466 510
Size: 466 44
PasI CC'CwG_GG
Cuts at: 0 45 159 182 510
Size: 45 114 23 328
Fragments arranged by size:
328 114 45 23
PfoI T'CCnGG_A
Cuts at: 0 398 406 510
Size: 398 8 104
Fragments arranged by size:
398 104 8
SapI GCTCTTCn'nnn_
Cuts at: 0 143 368 429 510
Size: 143 225 61 81
Fragments arranged by size:
225 143 81 61
SfoI GGC'GCC
Cuts at: 0 467 510
Size: 467 43
SgrAI Cr'CCGG_yG
Cuts at: 0 233 510
Size: 233 277
SmaI CCC'GGG
Cuts at: 0 131 258 510
Size: 131 127 252
Fragments arranged by size:
252 131 127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 150 375 510
Size: 150 225 135
Fragments arranged by size:
225 150 135
XmaI C'CCGG_G
Cuts at: 0 129 256 510
Size: 129 127 254
Fragments arranged by size:
254 129 127
Enzymes that do cut:
AhdI AvaI BamHI BanI BanII BbeI BglII BmgBI
BsaBI BsaHI BsaWI BseRI BsmBI Bsp1286I BspEI BsrBI
BsrDI BsrFI BstAPI BstYI EaeI EarI EcoNI FalI
HaeII Hin4I KasI MfeI MscI NarI PasI PfoI
SapI SfoI SgrAI SmaI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaXI
BseYI BsgI BsiEI BsiHKAI BsiWI BsmI BspHI BspMI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FseI
FspI FspAI HincII HindIII HpaI KpnI MluI MmeI
MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfcI SfiI SgfI SmlI SnaBI SpeI
SphI SrfI SspI StuI StyI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmnI ZraI