Restriction map for
pF1KE0594
(Linear) (Six-base) MAPSORT of: KIEE0594.seq Check: 3445 from: 1 to: 510
KIEE0594.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 83 510
Size: 83 427
AlwNI CAG_nnn'CTG
Cuts at: 0 446 510
Size: 446 64
AvaI C'yCGr_G
Cuts at: 0 34 81 129 256 510
Size: 34 47 48 127 254
Fragments arranged by size:
254 127 48 47 34
BamHI G'GATC_C
Cuts at: 0 307 510
Size: 307 203
BanI G'GyrC_C
Cuts at: 0 465 510
Size: 465 45
BanII G_rGCy'C
Cuts at: 0 182 510
Size: 182 328
BbeI G_GCGC'C
Cuts at: 0 469 510
Size: 469 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 456 510
Size: 456 54
BsaHI Gr'CG_yC
Cuts at: 0 466 510
Size: 466 44
BsaWI w'CCGG_w
Cuts at: 0 174 304 485 510
Size: 174 130 181 25
Fragments arranged by size:
181 174 130 25
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 316 510
Size: 316 194
BsmI GAATG_Cn'
Cuts at: 0 359 510
Size: 359 151
BsmBI CGTCTCn'nnnn_
Cuts at: 0 255 382 510
Size: 255 127 128
Fragments arranged by size:
255 128 127
Bsp1286I G_dGCh'C
Cuts at: 0 182 510
Size: 182 328
BspEI T'CCGG_A
Cuts at: 0 174 510
Size: 174 336
BsrBI CCG'CTC
Cuts at: 0 117 510
Size: 117 393
BsrDI GCAATG_nn'
Cuts at: 0 134 498 510
Size: 134 364 12
Fragments arranged by size:
364 134 12
BsrFI r'CCGG_y
Cuts at: 0 233 510
Size: 233 277
BstAPI GCAn_nnn'nTGC
Cuts at: 0 488 510
Size: 488 22
BstYI r'GATC_y
Cuts at: 0 307 510
Size: 307 203
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 107 510
Size: 107 403
EaeI y'GGCC_r
Cuts at: 0 380 510
Size: 380 130
EarI CTCTTCn'nnn_
Cuts at: 0 368 429 510
Size: 368 61 81
Fragments arranged by size:
368 81 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 34 510
Size: 34 476
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 366 510
Size: 366 144
HaeII r_GCGC'y
Cuts at: 0 469 510
Size: 469 41
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 69 101 510
Size: 69 32 409
Fragments arranged by size:
409 69 32
KasI G'GCGC_C
Cuts at: 0 465 510
Size: 465 45
MfeI C'AATT_G
Cuts at: 0 503 510
Size: 503 7
MscI TGG'CCA
Cuts at: 0 382 510
Size: 382 128
MspA1I CmG'CkG
Cuts at: 0 153 510
Size: 153 357
NarI GG'CG_CC
Cuts at: 0 466 510
Size: 466 44
PasI CC'CwG_GG
Cuts at: 0 45 159 182 510
Size: 45 114 23 328
Fragments arranged by size:
328 114 45 23
PflMI CCAn_nnn'nTGG
Cuts at: 0 446 510
Size: 446 64
PfoI T'CCnGG_A
Cuts at: 0 398 406 510
Size: 398 8 104
Fragments arranged by size:
398 104 8
PvuII CAG'CTG
Cuts at: 0 153 510
Size: 153 357
SapI GCTCTTCn'nnn_
Cuts at: 0 368 429 510
Size: 368 61 81
Fragments arranged by size:
368 81 61
SfoI GGC'GCC
Cuts at: 0 467 510
Size: 467 43
SgrAI Cr'CCGG_yG
Cuts at: 0 233 510
Size: 233 277
SmaI CCC'GGG
Cuts at: 0 131 258 510
Size: 131 127 252
Fragments arranged by size:
252 131 127
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 150 375 510
Size: 150 225 135
Fragments arranged by size:
225 150 135
XmaI C'CCGG_G
Cuts at: 0 129 256 510
Size: 129 127 254
Fragments arranged by size:
254 129 127
Enzymes that do cut:
AhdI AlwNI AvaI BamHI BanI BanII BbeI BmrI
BsaHI BsaWI BseRI BsmI BsmBI Bsp1286I BspEI BsrBI
BsrDI BsrFI BstAPI BstYI BtgZI EaeI EarI EcoNI
FalI HaeII Hin4I KasI MfeI MscI MspA1I NarI
PasI PflMI PfoI PvuII SapI SfoI SgrAI SmaI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaXI BseYI BsgI BsiEI BsiHKAI BsiWI BspHI BspMI
BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FseI FspI
FspAI HincII HindIII HpaI KpnI MluI MmeI MslI
NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI
NspI PacI PciI PmeI PmlI PpiI PpuMI PshAI
PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfcI SfiI
SgfI SmlI SnaBI SpeI SphI SrfI SspI StuI
StyI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmnI ZraI