Restriction map for
pF1KE0589
(Linear) (Six-base) MAPSORT of: KIEE0589.seq Check: 2319 from: 1 to: 1386
KIEE0589.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AflIII A'CryG_T
Cuts at: 0 893 1139 1386
Size: 893 246 247
Fragments arranged by size:
893 247 246
AlwNI CAG_nnn'CTG
Cuts at: 0 252 1386
Size: 252 1134
AscI GG'CGCG_CC
Cuts at: 0 544 1386
Size: 544 842
AvaI C'yCGr_G
Cuts at: 0 1049 1115 1386
Size: 1049 66 271
Fragments arranged by size:
1049 271 66
BamHI G'GATC_C
Cuts at: 0 261 1386
Size: 261 1125
BanI G'GyrC_C
Cuts at: 0 675 1110 1386
Size: 675 435 276
Fragments arranged by size:
675 435 276
BanII G_rGCy'C
Cuts at: 0 948 1386
Size: 948 438
BbeI G_GCGC'C
Cuts at: 0 1114 1386
Size: 1114 272
BbsI GAAGACnn'nnnn_
Cuts at: 0 107 1113 1386
Size: 107 1006 273
Fragments arranged by size:
1006 273 107
BbvCI CC'TCA_GC
Cuts at: 0 166 1386
Size: 166 1220
BlpI GC'TnA_GC
Cuts at: 0 406 1386
Size: 406 980
Bme1580I G_kGCm'C
Cuts at: 0 680 1386
Size: 680 706
BmgBI CAC'GTC
Cuts at: 0 396 1386
Size: 396 990
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 730 1188 1386
Size: 210 520 458 198
Fragments arranged by size:
520 458 210 198
Bpu10I CC'TnA_GC
Cuts at: 0 166 1386
Size: 166 1220
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 922 1386
Size: 922 464
BsaI GGTCTCn'nnnn_
Cuts at: 0 176 1386
Size: 176 1210
BsaAI yAC'GTr
Cuts at: 0 1146 1386
Size: 1146 240
BsaBI GATnn'nnATC
Cuts at: 0 745 1386
Size: 745 641
BsaHI Gr'CG_yC
Cuts at: 0 467 1111 1386
Size: 467 644 275
Fragments arranged by size:
644 467 275
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1038 1386
Size: 1038 348
BseYI C'CCAG_C
Cuts at: 0 194 498 1386
Size: 194 304 888
Fragments arranged by size:
888 304 194
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 775 1290 1386
Size: 775 515 96
Fragments arranged by size:
775 515 96
BsiHKAI G_wGCw'C
Cuts at: 0 1066 1386
Size: 1066 320
BsmI GAATG_Cn'
Cuts at: 0 138 1386
Size: 138 1248
Bsp1286I G_dGCh'C
Cuts at: 0 680 948 1066 1386
Size: 680 268 118 320
Fragments arranged by size:
680 320 268 118
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1307 1386
Size: 1307 79
BsrDI GCAATG_nn'
Cuts at: 0 833 1386
Size: 833 553
BsrFI r'CCGG_y
Cuts at: 0 865 1013 1386
Size: 865 148 373
Fragments arranged by size:
865 373 148
BssHII G'CGCG_C
Cuts at: 0 544 1386
Size: 544 842
BssSI C'ACGA_G
Cuts at: 0 368 1386
Size: 368 1018
BstEII G'GTnAC_C
Cuts at: 0 990 1386
Size: 990 396
BstXI CCAn_nnnn'nTGG
Cuts at: 0 708 924 1077 1203 1386
Size: 708 216 153 126 183
Fragments arranged by size:
708 216 183 153 126
BstYI r'GATC_y
Cuts at: 0 32 261 1386
Size: 32 229 1125
Fragments arranged by size:
1125 229 32
BtgI C'CryG_G
Cuts at: 0 20 1386
Size: 20 1366
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 329 1386
Size: 329 1057
BtsI GCAGTG_nn'
Cuts at: 0 65 539 665 770 1386
Size: 65 474 126 105 616
Fragments arranged by size:
616 474 126 105 65
DrdI GACnn_nn'nnGTC
Cuts at: 0 1171 1386
Size: 1171 215
EaeI y'GGCC_r
Cuts at: 0 360 430 698 1001 1193 1205 1386
Size: 360 70 268 303 192 12 181
Fragments arranged by size:
360 303 268 192 181 70 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 226 1386
Size: 226 1160
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 210 730 1188 1386
Size: 210 520 458 198
Fragments arranged by size:
520 458 210 198
EcoNI CCTnn'n_nnAGG
Cuts at: 0 649 1386
Size: 649 737
EcoO109I rG'GnC_Cy
Cuts at: 0 42 198 337 449 569 933 1386
Size: 42 156 139 112 120 364 453
Fragments arranged by size:
453 364 156 139 120 112 42
EcoRV GAT'ATC
Cuts at: 0 609 1386
Size: 609 777
FspI TGC'GCA
Cuts at: 0 694 1386
Size: 694 692
FspAI rTGC'GCAy
Cuts at: 0 694 1386
Size: 694 692
HaeII r_GCGC'y
Cuts at: 0 595 1114 1386
Size: 595 519 272
Fragments arranged by size:
595 519 272
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 895 927 1268 1300 1341 1373 1386
Size: 895 32 341 32 41 32 13
Fragments arranged by size:
895 341 41 32 32 32 13
HincII GTy'rAC
Cuts at: 0 762 863 1386
Size: 762 101 523
Fragments arranged by size:
762 523 101
KasI G'GCGC_C
Cuts at: 0 1110 1386
Size: 1110 276
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1299 1386
Size: 1299 87
MscI TGG'CCA
Cuts at: 0 362 432 1195 1207 1386
Size: 362 70 763 12 179
Fragments arranged by size:
763 362 179 70 12
MslI CAynn'nnrTG
Cuts at: 0 619 1144 1386
Size: 619 525 242
Fragments arranged by size:
619 525 242
MspA1I CmG'CkG
Cuts at: 0 310 358 401 1386
Size: 310 48 43 985
Fragments arranged by size:
985 310 48 43
NarI GG'CG_CC
Cuts at: 0 1111 1386
Size: 1111 275
NcoI C'CATG_G
Cuts at: 0 20 1386
Size: 20 1366
NspI r_CATG'y
Cuts at: 0 897 1143 1386
Size: 897 246 243
Fragments arranged by size:
897 246 243
PciI A'CATG_T
Cuts at: 0 893 1139 1386
Size: 893 246 247
Fragments arranged by size:
893 247 246
PflMI CCAn_nnn'nTGG
Cuts at: 0 687 1386
Size: 687 699
PfoI T'CCnGG_A
Cuts at: 0 707 1359 1386
Size: 707 652 27
Fragments arranged by size:
707 652 27
PpuMI rG'GwC_Cy
Cuts at: 0 42 198 337 449 933 1386
Size: 42 156 139 112 484 453
Fragments arranged by size:
484 453 156 139 112 42
PstI C_TGCA'G
Cuts at: 0 59 653 1046 1386
Size: 59 594 393 340
Fragments arranged by size:
594 393 340 59
PvuII CAG'CTG
Cuts at: 0 310 358 1386
Size: 310 48 1028
Fragments arranged by size:
1028 310 48
SanDI GG'GwC_CC
Cuts at: 0 42 337 1386
Size: 42 295 1049
Fragments arranged by size:
1049 295 42
SbfI CC_TGCA'GG
Cuts at: 0 59 653 1386
Size: 59 594 733
Fragments arranged by size:
733 594 59
SfcI C'TryA_G
Cuts at: 0 55 486 649 1042 1386
Size: 55 431 163 393 344
Fragments arranged by size:
431 393 344 163 55
SfoI GGC'GCC
Cuts at: 0 1112 1386
Size: 1112 274
SmaI CCC'GGG
Cuts at: 0 1051 1117 1386
Size: 1051 66 269
Fragments arranged by size:
1051 269 66
SmlI C'TyrA_G
Cuts at: 0 937 1386
Size: 937 449
StyI C'CwwG_G
Cuts at: 0 20 218 291 1184 1386
Size: 20 198 73 893 202
Fragments arranged by size:
893 202 198 73 20
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 930 1386
Size: 930 456
Tth111I GACn'n_nGTC
Cuts at: 0 931 1386
Size: 931 455
XmaI C'CCGG_G
Cuts at: 0 1049 1115 1386
Size: 1049 66 271
Fragments arranged by size:
1049 271 66
Enzymes that do cut:
AflIII AlwNI AscI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaI BsaAI BsaBI BsaHI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspMI BsrDI BsrFI BssHII BssSI BstEII
BstXI BstYI BtgI BtgZI BtsI DrdI EaeI EciI
Eco57MI EcoNI EcoO109I EcoRV FspI FspAI HaeII Hin4I
HincII KasI MmeI MscI MslI MspA1I NarI NcoI
NspI PciI PflMI PfoI PpuMI PstI PvuII SanDI
SbfI SfcI SfoI SmaI SmlI StyI TaqII Tth111I
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI
AseI AsiSI AvrII BaeI BcgI BciVI BclI BfrBI
BglI BglII BmrI BmtI BplI BsaWI BsaXI BsiEI
BsiWI BsmBI BspEI BspHI BsrBI BsrGI BstAPI BstBI
BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI EarI
EcoICRI EcoRI FalI FseI HindIII HpaI KpnI MfeI
MluI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SapI
ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI TatI TstI XbaI XcmI
XhoI XmnI ZraI