Restriction map for
pF1KE0585
(Linear) (Six-base) MAPSORT of: KIEE0585.seq Check: 1933 from: 1 to: 906
KIEE0585.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AatII G_ACGT'C
Cuts at: 0 854 906
Size: 854 52
AccI GT'mk_AC
Cuts at: 0 672 906
Size: 672 234
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 761 906
Size: 761 145
AflII C'TTAA_G
Cuts at: 0 256 906
Size: 256 650
AflIII A'CryG_T
Cuts at: 0 605 906
Size: 605 301
AhdI GACnn_n'nnGTC
Cuts at: 0 657 850 906
Size: 657 193 56
Fragments arranged by size:
657 193 56
AleI CACnn'nnGTG
Cuts at: 0 517 906
Size: 517 389
AlwNI CAG_nnn'CTG
Cuts at: 0 346 514 906
Size: 346 168 392
Fragments arranged by size:
392 346 168
ApoI r'AATT_y
Cuts at: 0 723 906
Size: 723 183
BciVI GTATCCnnnnn_n'
Cuts at: 0 628 906
Size: 628 278
BclI T'GATC_A
Cuts at: 0 365 906
Size: 365 541
BfrBI ATG'CAT
Cuts at: 0 295 456 474 885 889 906
Size: 295 161 18 411 4 17
Fragments arranged by size:
411 295 161 18 17 4
BglII A'GATC_T
Cuts at: 0 249 534 906
Size: 249 285 372
Fragments arranged by size:
372 285 249
BmrI ACTGGGnnnn_n'
Cuts at: 0 524 906
Size: 524 382
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 906
Size: 596 310
BsaI GGTCTCn'nnnn_
Cuts at: 0 665 906
Size: 665 241
BsaAI yAC'GTr
Cuts at: 0 226 906
Size: 226 680
BsaHI Gr'CG_yC
Cuts at: 0 851 906
Size: 851 55
BsaWI w'CCGG_w
Cuts at: 0 69 906
Size: 69 837
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 161 191 906
Size: 161 30 715
Fragments arranged by size:
715 161 30
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 214 560 906
Size: 214 346 346
Fragments arranged by size:
346 346 214
BsiEI CG_ry'CG
Cuts at: 0 70 906
Size: 70 836
BsmI GAATG_Cn'
Cuts at: 0 26 456 906
Size: 26 430 450
Fragments arranged by size:
450 430 26
BsrFI r'CCGG_y
Cuts at: 0 548 906
Size: 548 358
BstAPI GCAn_nnn'nTGC
Cuts at: 0 895 906
Size: 895 11
BstBI TT'CG_AA
Cuts at: 0 102 906
Size: 102 804
BstXI CCAn_nnnn'nTGG
Cuts at: 0 519 906
Size: 519 387
BstYI r'GATC_y
Cuts at: 0 249 534 831 906
Size: 249 285 297 75
Fragments arranged by size:
297 285 249 75
BtsI GCAGTG_nn'
Cuts at: 0 444 893 906
Size: 444 449 13
Fragments arranged by size:
449 444 13
DraI TTT'AAA
Cuts at: 0 185 906
Size: 185 721
EaeI y'GGCC_r
Cuts at: 0 546 906
Size: 546 360
EarI CTCTTCn'nnn_
Cuts at: 0 459 776 906
Size: 459 317 130
Fragments arranged by size:
459 317 130
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 761 906
Size: 596 165 145
Fragments arranged by size:
596 165 145
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 643 675 836 868 906
Size: 27 616 32 161 32 38
Fragments arranged by size:
616 161 38 32 32 27
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 825 906
Size: 72 753 81
Fragments arranged by size:
753 81 72
MslI CAynn'nnrTG
Cuts at: 0 275 517 629 906
Size: 275 242 112 277
Fragments arranged by size:
277 275 242 112
MspA1I CmG'CkG
Cuts at: 0 574 906
Size: 574 332
NsiI A_TGCA'T
Cuts at: 0 297 458 476 887 891 906
Size: 297 161 18 411 4 15
Fragments arranged by size:
411 297 161 18 15 4
NspI r_CATG'y
Cuts at: 0 456 609 889 893 906
Size: 456 153 280 4 13
Fragments arranged by size:
456 280 153 13 4
PciI A'CATG_T
Cuts at: 0 605 906
Size: 605 301
PmlI CAC'GTG
Cuts at: 0 226 906
Size: 226 680
PvuII CAG'CTG
Cuts at: 0 574 906
Size: 574 332
SmlI C'TyrA_G
Cuts at: 0 256 906
Size: 256 650
SphI G_CATG'C
Cuts at: 0 889 893 906
Size: 889 4 13
Fragments arranged by size:
889 13 4
SspI AAT'ATT
Cuts at: 0 289 906
Size: 289 617
StuI AGG'CCT
Cuts at: 0 61 906
Size: 61 845
ZraI GAC'GTC
Cuts at: 0 852 906
Size: 852 54
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AhdI AleI AlwNI
ApoI BciVI BclI BfrBI BglII BmrI BpmI BsaI
BsaAI BsaHI BsaWI BsaXI BsgI BsiEI BsmI BsrFI
BstAPI BstBI BstXI BstYI BtsI DraI EaeI EarI
Eco57MI Hin4I MmeI MslI MspA1I NsiI NspI PciI
PmlI PvuII SmlI SphI SspI StuI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AlfI AloI ApaI
ApaLI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbsI BbvCI BcgI BglI BlpI
Bme1580I BmgBI BmtI BplI Bpu10I BpuEI BsaBI BseRI
BseYI BsiHKAI BsiWI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstEII BstZ17I Bsu36I
BtgI BtgZI ClaI CspCI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI PacI PasI PflMI PfoI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SrfI StyI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI