Restriction map for
pF1KE0584
(Linear) (Six-base) MAPSORT of: KIEE0584.seq Check: 6386 from: 1 to: 498
KIEE0584.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 170 498
Size: 170 328
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 222 498
Size: 222 276
AleI CACnn'nnGTG
Cuts at: 0 493 498
Size: 493 5
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 208 498
Size: 208 290
AvaI C'yCGr_G
Cuts at: 0 402 498
Size: 402 96
BanII G_rGCy'C
Cuts at: 0 296 498
Size: 296 202
BmgBI CAC'GTC
Cuts at: 0 339 390 495 498
Size: 339 51 105 3
Fragments arranged by size:
339 105 51 3
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 498
Size: 73 425
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 136 145 340 498
Size: 136 9 195 158
Fragments arranged by size:
195 158 136 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 498
Size: 157 341
BsiHKAI G_wGCw'C
Cuts at: 0 365 498
Size: 365 133
Bsp1286I G_dGCh'C
Cuts at: 0 296 365 498
Size: 296 69 133
Fragments arranged by size:
296 133 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 170 498
Size: 170 328
BsrFI r'CCGG_y
Cuts at: 0 83 207 498
Size: 83 124 291
Fragments arranged by size:
291 124 83
BtgI C'CryG_G
Cuts at: 0 14 498
Size: 14 484
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 48 498
Size: 48 450
BtsI GCAGTG_nn'
Cuts at: 0 285 498
Size: 285 213
EarI CTCTTCn'nnn_
Cuts at: 0 326 498
Size: 326 172
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 222 498
Size: 73 149 276
Fragments arranged by size:
276 149 73
EcoNI CCTnn'n_nnAGG
Cuts at: 0 468 498
Size: 468 30
EcoO109I rG'GnC_Cy
Cuts at: 0 417 498
Size: 417 81
HaeII r_GCGC'y
Cuts at: 0 214 430 498
Size: 214 216 68
Fragments arranged by size:
216 214 68
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 98 203 498
Size: 98 105 295
Fragments arranged by size:
295 105 98
MslI CAynn'nnrTG
Cuts at: 0 493 498
Size: 493 5
MspA1I CmG'CkG
Cuts at: 0 171 278 498
Size: 171 107 220
Fragments arranged by size:
220 171 107
NaeI GCC'GGC
Cuts at: 0 85 209 498
Size: 85 124 289
Fragments arranged by size:
289 124 85
NgoMIV G'CCGG_C
Cuts at: 0 83 207 498
Size: 83 124 291
Fragments arranged by size:
291 124 83
PshAI GACnn'nnGTC
Cuts at: 0 16 498
Size: 16 482
PstI C_TGCA'G
Cuts at: 0 314 425 498
Size: 314 111 73
Fragments arranged by size:
314 111 73
PvuII CAG'CTG
Cuts at: 0 278 498
Size: 278 220
SbfI CC_TGCA'GG
Cuts at: 0 314 425 498
Size: 314 111 73
Fragments arranged by size:
314 111 73
ScaI AGT'ACT
Cuts at: 0 94 498
Size: 94 404
SexAI A'CCwGG_T
Cuts at: 0 188 498
Size: 188 310
SfcI C'TryA_G
Cuts at: 0 310 421 498
Size: 310 111 77
Fragments arranged by size:
310 111 77
SmaI CCC'GGG
Cuts at: 0 404 498
Size: 404 94
StuI AGG'CCT
Cuts at: 0 463 498
Size: 463 35
TatI w'GTAC_w
Cuts at: 0 92 498
Size: 92 406
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 186 498
Size: 186 312
XmaI C'CCGG_G
Cuts at: 0 402 498
Size: 402 96
Enzymes that do cut:
AarI AcuI AleI AlfI AvaI BanII BmgBI BpmI
BseRI BsgI BsiHKAI Bsp1286I BspMI BsrFI BtgI BtgZI
BtsI EarI Eco57MI EcoNI EcoO109I HaeII MmeI MslI
MspA1I NaeI NgoMIV PshAI PstI PvuII SbfI ScaI
SexAI SfcI SmaI StuI TatI XcmI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BanI BbeI BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmrI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspHI BsrBI BsrDI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI
DraI DraIII DrdI EaeI EagI EciI EcoICRI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI KasI KpnI MfeI MluI MscI NarI NcoI
NdeI NheI NotI NruI NsiI NspI PacI PasI
PciI PflMI PfoI PmeI PmlI PpiI PpuMI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StyI SwaI TaqII TstI
Tth111I XbaI XhoI XmnI ZraI