(Linear) (Six-base) MAPSORT of: KIEE0580.seq  Check: 1416  from: 1  to: 468



KIEE0580.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     468 
   Size:         59     409
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     121     468 
   Size:        121     347
 
BglI GCCn_nnn'nGGC

Cuts at:      0      76     468 
   Size:         76     392
 
BmgBI CAC'GTC

Cuts at:      0     125     468 
   Size:        125     343
 
BsaHI Gr'CG_yC

Cuts at:      0     159     468 
   Size:        159     309
 
BsiEI CG_ry'CG

Cuts at:      0     257     468 
   Size:        257     211
 
BsiHKAI G_wGCw'C

Cuts at:      0     193     468 
   Size:        193     275
 
Bsp1286I G_dGCh'C

Cuts at:      0     193     468 
   Size:        193     275
 
BstEII G'GTnAC_C

Cuts at:      0     369     468 
   Size:        369      99
 
BtgI C'CryG_G

Cuts at:      0     245     468 
   Size:        245     223
 
BtsI GCAGTG_nn'

Cuts at:      0     151     468 
   Size:        151     317
 
DraI TTT'AAA

Cuts at:      0      65     468 
   Size:         65     403
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     123     249     468 
   Size:        123     126     219

  Fragments arranged by size: 

                 219     126     123
 
EaeI y'GGCC_r

Cuts at:      0     213     254     362     468 
   Size:        213      41     108     106

  Fragments arranged by size: 

                 213     108     106      41
 
EagI C'GGCC_G

Cuts at:      0     254     468 
   Size:        254     214
 
EarI CTCTTCn'nnn_

Cuts at:      0     181     256     468 
   Size:        181      75     212

  Fragments arranged by size: 

                 212     181      75
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     374     468 
   Size:        374      94
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      59     468 
   Size:         59     409
 
HaeII r_GCGC'y

Cuts at:      0       7     468 
   Size:          7     461
 
HindIII A'AGCT_T

Cuts at:      0     208     468 
   Size:        208     260
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     419     468 
   Size:        419      49
 
MscI TGG'CCA

Cuts at:      0     215     468 
   Size:        215     253
 
MspA1I CmG'CkG

Cuts at:      0     141     240     468 
   Size:        141      99     228

  Fragments arranged by size: 

                 228     141      99
 
NspI r_CATG'y

Cuts at:      0     410     468 
   Size:        410      58
 
SapI GCTCTTCn'nnn_

Cuts at:      0     181     468 
   Size:        181     287
 
StyI C'CwwG_G

Cuts at:      0     181     468 
   Size:        181     287
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     119     151     366     398     468 
   Size:        119      32     215      32      70

  Fragments arranged by size: 

                 215     119      70      32      32
 

 Enzymes that do cut:

     AcuI     BbsI     BglI    BmgBI    BsaHI    BsiEI  BsiHKAI Bsp1286I
   BstEII     BtgI     BtsI     DraI     DrdI     EaeI     EagI     EarI
     EciI  Eco57MI    HaeII  HindIII     MmeI     MscI   MspA1I     NspI
     SapI     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI
    BglII     BlpI Bme1580I     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaWI    BsaXI    BseRI    BseYI
     BsgI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI    BstXI
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI   DraIII  EcoICRI
    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    Hin4I   HincII     HpaI     KasI     KpnI     MfeI     MluI     MslI
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI
    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI