Restriction map for
pF1KE0576
(Linear) (Six-base) MAPSORT of: KIEE0576.seq Check: 6345 from: 1 to: 813
KIEE0576.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 258 289 813
Size: 258 31 524
Fragments arranged by size:
524 258 31
ApaI G_GGCC'C
Cuts at: 0 465 813
Size: 465 348
ApoI r'AATT_y
Cuts at: 0 352 760 813
Size: 352 408 53
Fragments arranged by size:
408 352 53
BanII G_rGCy'C
Cuts at: 0 465 813
Size: 465 348
BfrBI ATG'CAT
Cuts at: 0 778 813
Size: 778 35
BlpI GC'TnA_GC
Cuts at: 0 362 813
Size: 362 451
Bme1580I G_kGCm'C
Cuts at: 0 465 813
Size: 465 348
BsaI GGTCTCn'nnnn_
Cuts at: 0 622 813
Size: 622 191
BsaWI w'CCGG_w
Cuts at: 0 218 813
Size: 218 595
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 813
Size: 19 30 764
Fragments arranged by size:
764 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 430 813
Size: 430 383
BseYI C'CCAG_C
Cuts at: 0 565 710 813
Size: 565 145 103
Fragments arranged by size:
565 145 103
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 519 769 813
Size: 519 250 44
Fragments arranged by size:
519 250 44
BsiHKAI G_wGCw'C
Cuts at: 0 368 440 813
Size: 368 72 373
Fragments arranged by size:
373 368 72
Bsp1286I G_dGCh'C
Cuts at: 0 368 440 465 813
Size: 368 72 25 348
Fragments arranged by size:
368 348 72 25
BsrBI CCG'CTC
Cuts at: 0 30 134 813
Size: 30 104 679
Fragments arranged by size:
679 104 30
BsrDI GCAATG_nn'
Cuts at: 0 561 735 813
Size: 561 174 78
Fragments arranged by size:
561 174 78
BstAPI GCAn_nnn'nTGC
Cuts at: 0 610 813
Size: 610 203
BstEII G'GTnAC_C
Cuts at: 0 345 813
Size: 345 468
BstXI CCAn_nnnn'nTGG
Cuts at: 0 357 813
Size: 357 456
BstYI r'GATC_y
Cuts at: 0 548 813
Size: 548 265
Bsu36I CC'TnA_GG
Cuts at: 0 442 813
Size: 442 371
BtgI C'CryG_G
Cuts at: 0 560 813
Size: 560 253
DrdI GACnn_nn'nnGTC
Cuts at: 0 157 813
Size: 157 656
EarI CTCTTCn'nnn_
Cuts at: 0 691 813
Size: 691 122
EcoO109I rG'GnC_Cy
Cuts at: 0 461 813
Size: 461 352
HaeII r_GCGC'y
Cuts at: 0 291 813
Size: 291 522
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 3 35 813
Size: 3 32 778
Fragments arranged by size:
778 32 3
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 813
Size: 67 746
MslI CAynn'nnrTG
Cuts at: 0 371 813
Size: 371 442
NcoI C'CATG_G
Cuts at: 0 560 813
Size: 560 253
NsiI A_TGCA'T
Cuts at: 0 780 813
Size: 780 33
PasI CC'CwG_GG
Cuts at: 0 652 813
Size: 652 161
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 62 94 813
Size: 62 32 719
Fragments arranged by size:
719 62 32
PspOMI G'GGCC_C
Cuts at: 0 461 813
Size: 461 352
PstI C_TGCA'G
Cuts at: 0 591 813
Size: 591 222
SbfI CC_TGCA'GG
Cuts at: 0 591 813
Size: 591 222
SexAI A'CCwGG_T
Cuts at: 0 301 813
Size: 301 512
SfcI C'TryA_G
Cuts at: 0 587 813
Size: 587 226
StuI AGG'CCT
Cuts at: 0 636 813
Size: 636 177
StyI C'CwwG_G
Cuts at: 0 560 751 813
Size: 560 191 62
Fragments arranged by size:
560 191 62
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 423 813
Size: 423 390
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 749 813
Size: 749 64
Enzymes that do cut:
AlfI ApaI ApoI BanII BfrBI BlpI Bme1580I BsaI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrBI
BsrDI BstAPI BstEII BstXI BstYI Bsu36I BtgI DrdI
EarI EcoO109I HaeII Hin4I MmeI MslI NcoI NsiI
PasI PpiI PspOMI PstI SbfI SexAI SfcI StuI
StyI TaqII XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AloI AlwNI ApaLI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BbeI
BbsI BbvCI BcgI BciVI BclI BglI BglII BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaAI BsaBI
BsaHI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrFI BsrGI BssHII BssSI BstBI BstZ17I BtgZI BtsI
ClaI CspCI DraI DraIII EaeI EagI EciI EcoICRI
Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI
HincII HindIII HpaI KasI KpnI MfeI MluI MscI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NspI PacI PciI PflMI PfoI PmeI PmlI PpuMI
PshAI PsiI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI
ZraI