Restriction map for
pF1KE0573
(Linear) (Six-base) MAPSORT of: KIEE0573.seq Check: 9742 from: 1 to: 867
KIEE0573.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AccI GT'mk_AC
Cuts at: 0 446 867
Size: 446 421
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 483 867
Size: 483 384
AlwNI CAG_nnn'CTG
Cuts at: 0 73 258 372 506 867
Size: 73 185 114 134 361
Fragments arranged by size:
361 185 134 114 73
AvaI C'yCGr_G
Cuts at: 0 12 412 867
Size: 12 400 455
Fragments arranged by size:
455 400 12
BanI G'GyrC_C
Cuts at: 0 352 735 867
Size: 352 383 132
Fragments arranged by size:
383 352 132
BanII G_rGCy'C
Cuts at: 0 379 475 598 828 867
Size: 379 96 123 230 39
Fragments arranged by size:
379 230 123 96 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 182 867
Size: 182 685
BglI GCCn_nnn'nGGC
Cuts at: 0 761 867
Size: 761 106
BlpI GC'TnA_GC
Cuts at: 0 373 867
Size: 373 494
Bme1580I G_kGCm'C
Cuts at: 0 241 355 738 867
Size: 241 114 383 129
Fragments arranged by size:
383 241 129 114
BmgBI CAC'GTC
Cuts at: 0 858 867
Size: 858 9
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 38 867
Size: 38 829
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 235 280 302 508 711 742 867
Size: 37 198 45 22 206 203 31 125
Fragments arranged by size:
206 203 198 125 45 37 31 22
BsaI GGTCTCn'nnnn_
Cuts at: 0 66 97 376 867
Size: 66 31 279 491
Fragments arranged by size:
491 279 66 31
BsaAI yAC'GTr
Cuts at: 0 450 867
Size: 450 417
BsaHI Gr'CG_yC
Cuts at: 0 307 799 814 867
Size: 307 492 15 53
Fragments arranged by size:
492 307 53 15
BsaWI w'CCGG_w
Cuts at: 0 794 867
Size: 794 73
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 457 487 709 739 867
Size: 457 30 222 30 128
Fragments arranged by size:
457 222 128 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 54 160 169 361 430 496 867
Size: 54 106 9 192 69 66 371
Fragments arranged by size:
371 192 106 69 66 54 9
BseYI C'CCAG_C
Cuts at: 0 86 867
Size: 86 781
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 346 622 867
Size: 346 276 245
Fragments arranged by size:
346 276 245
BsiEI CG_ry'CG
Cuts at: 0 362 832 867
Size: 362 470 35
Fragments arranged by size:
470 362 35
Bsp1286I G_dGCh'C
Cuts at: 0 241 355 379 475 598 738 828 867
Size: 241 114 24 96 123 140 90 39
Fragments arranged by size:
241 140 123 114 96 90 39 24
BsrBI CCG'CTC
Cuts at: 0 20 867
Size: 20 847
BsrDI GCAATG_nn'
Cuts at: 0 241 867
Size: 241 626
BsrFI r'CCGG_y
Cuts at: 0 801 867
Size: 801 66
BstYI r'GATC_y
Cuts at: 0 439 867
Size: 439 428
Bsu36I CC'TnA_GG
Cuts at: 0 74 867
Size: 74 793
BtgI C'CryG_G
Cuts at: 0 287 310 356 386 611 699 867
Size: 287 23 46 30 225 88 168
Fragments arranged by size:
287 225 168 88 46 30 23
EaeI y'GGCC_r
Cuts at: 0 359 829 867
Size: 359 470 38
Fragments arranged by size:
470 359 38
EagI C'GGCC_G
Cuts at: 0 359 829 867
Size: 359 470 38
Fragments arranged by size:
470 359 38
EarI CTCTTCn'nnn_
Cuts at: 0 31 155 338 637 867
Size: 31 124 183 299 230
Fragments arranged by size:
299 230 183 124 31
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 465 867
Size: 465 402
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 235 280 302 483 508 711 742
Size: 37 198 45 22 181 25 203 31
Cuts at: 742 867
Size: 125
Fragments arranged by size:
203 198 181 125 45 37 31 25
22
EcoO109I rG'GnC_Cy
Cuts at: 0 92 407 705 767 867
Size: 92 315 298 62 100
Fragments arranged by size:
315 298 100 92 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 424 456 515 532 547 564 867
Size: 424 32 59 17 15 17 303
Fragments arranged by size:
424 303 59 32 17 17 15
MslI CAynn'nnrTG
Cuts at: 0 271 652 867
Size: 271 381 215
Fragments arranged by size:
381 271 215
MspA1I CmG'CkG
Cuts at: 0 358 369 506 701 752 834 867
Size: 358 11 137 195 51 82 33
Fragments arranged by size:
358 195 137 82 51 33 11
NaeI GCC'GGC
Cuts at: 0 803 867
Size: 803 64
NcoI C'CATG_G
Cuts at: 0 287 867
Size: 287 580
NgoMIV G'CCGG_C
Cuts at: 0 801 867
Size: 801 66
PasI CC'CwG_GG
Cuts at: 0 81 679 839 867
Size: 81 598 160 28
Fragments arranged by size:
598 160 81 28
PflMI CCAn_nnn'nTGG
Cuts at: 0 130 258 867
Size: 130 128 609
Fragments arranged by size:
609 130 128
PfoI T'CCnGG_A
Cuts at: 0 318 867
Size: 318 549
PpuMI rG'GwC_Cy
Cuts at: 0 407 867
Size: 407 460
PvuII CAG'CTG
Cuts at: 0 369 867
Size: 369 498
SacII CC_GC'GG
Cuts at: 0 359 702 867
Size: 359 343 165
Fragments arranged by size:
359 343 165
SanDI GG'GwC_CC
Cuts at: 0 407 867
Size: 407 460
SgrAI Cr'CCGG_yG
Cuts at: 0 801 867
Size: 801 66
StuI AGG'CCT
Cuts at: 0 46 867
Size: 46 821
StyI C'CwwG_G
Cuts at: 0 107 287 560 590 867
Size: 107 180 273 30 277
Fragments arranged by size:
277 273 180 107 30
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 734 831 867
Size: 734 97 36
Fragments arranged by size:
734 97 36
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 709 741 867
Size: 709 32 126
Fragments arranged by size:
709 126 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 114 867
Size: 114 753
Enzymes that do cut:
AccI AcuI AlwNI AvaI BanI BanII BbsI BglI
BlpI Bme1580I BmgBI BplI BpmI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiEI Bsp1286I BsrBI
BsrDI BsrFI BstYI Bsu36I BtgI EaeI EagI EarI
EciI Eco57MI EcoO109I Hin4I MslI MspA1I NaeI NcoI
NgoMIV PasI PflMI PfoI PpuMI PvuII SacII SanDI
SgrAI StuI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI
BciVI BclI BfrBI BglII BmrI BmtI Bpu10I BpuEI
BsaBI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI KpnI MfeI MluI MmeI
MscI NarI NdeI NheI NotI NruI NsiI NspI
PacI PciI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SalI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI SwaI TatI
Tth111I XbaI XhoI XmaI XmnI ZraI