Restriction map for
pF1KE0572
(Linear) (Six-base) MAPSORT of: KIEE0572.seq Check: 7141 from: 1 to: 759
KIEE0572.orf
With 182 enzymes: SgfI *
April 24, 2009 13:16 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 127 555 755 759
Size: 127 428 200 4
Fragments arranged by size:
428 200 127 4
ApaI G_GGCC'C
Cuts at: 0 571 759
Size: 571 188
AvaI C'yCGr_G
Cuts at: 0 85 759
Size: 85 674
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 294 327 759
Size: 294 33 432
Fragments arranged by size:
432 294 33
BanI G'GyrC_C
Cuts at: 0 3 759
Size: 3 756
BanII G_rGCy'C
Cuts at: 0 61 248 344 571 759
Size: 61 187 96 227 188
Fragments arranged by size:
227 188 187 96 61
BbsI GAAGACnn'nnnn_
Cuts at: 0 684 759
Size: 684 75
BclI T'GATC_A
Cuts at: 0 131 759
Size: 131 628
BglI GCCn_nnn'nGGC
Cuts at: 0 329 759
Size: 329 430
BlpI GC'TnA_GC
Cuts at: 0 116 759
Size: 116 643
Bme1580I G_kGCm'C
Cuts at: 0 571 759
Size: 571 188
BmrI ACTGGGnnnn_n'
Cuts at: 0 401 530 641 759
Size: 401 129 111 118
Fragments arranged by size:
401 129 118 111
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 141 759
Size: 141 618
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 262 376 427 679 759
Size: 196 66 114 51 252 80
Fragments arranged by size:
252 196 114 80 66 51
BsaI GGTCTCn'nnnn_
Cuts at: 0 17 488 675 759
Size: 17 471 187 84
Fragments arranged by size:
471 187 84 17
BsaHI Gr'CG_yC
Cuts at: 0 50 759
Size: 50 709
BsaWI w'CCGG_w
Cuts at: 0 158 666 759
Size: 158 508 93
Fragments arranged by size:
508 158 93
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 38 68 419 449 490 520 759
Size: 38 30 351 30 41 30 239
Fragments arranged by size:
351 239 41 38 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 59 759
Size: 59 700
BseYI C'CCAG_C
Cuts at: 0 343 580 759
Size: 343 237 179
Fragments arranged by size:
343 237 179
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 8 759
Size: 8 751
BsiHKAI G_wGCw'C
Cuts at: 0 290 759
Size: 290 469
BsmBI CGTCTCn'nnnn_
Cuts at: 0 41 759
Size: 41 718
Bsp1286I G_dGCh'C
Cuts at: 0 61 248 290 344 571 759
Size: 61 187 42 54 227 188
Fragments arranged by size:
227 188 187 61 54 42
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 621 759
Size: 621 138
BstAPI GCAn_nnn'nTGC
Cuts at: 0 627 755 759
Size: 627 128 4
Fragments arranged by size:
627 128 4
BstEII G'GTnAC_C
Cuts at: 0 417 759
Size: 417 342
BstXI CCAn_nnnn'nTGG
Cuts at: 0 221 414 759
Size: 221 193 345
Fragments arranged by size:
345 221 193
Bsu36I CC'TnA_GG
Cuts at: 0 423 759
Size: 423 336
BtgI C'CryG_G
Cuts at: 0 7 759
Size: 7 752
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 196 262 376 427 679 759
Size: 196 66 114 51 252 80
Fragments arranged by size:
252 196 114 80 66 51
EcoO109I rG'GnC_Cy
Cuts at: 0 170 552 568 759
Size: 170 382 16 191
Fragments arranged by size:
382 191 170 16
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 254 286 759
Size: 254 32 473
Fragments arranged by size:
473 254 32
MspA1I CmG'CkG
Cuts at: 0 30 308 528 759
Size: 30 278 220 231
Fragments arranged by size:
278 231 220 30
NcoI C'CATG_G
Cuts at: 0 7 759
Size: 7 752
PasI CC'CwG_GG
Cuts at: 0 221 759
Size: 221 538
PflMI CCAn_nnn'nTGG
Cuts at: 0 110 759
Size: 110 649
PfoI T'CCnGG_A
Cuts at: 0 508 656 759
Size: 508 148 103
Fragments arranged by size:
508 148 103
PpuMI rG'GwC_Cy
Cuts at: 0 170 759
Size: 170 589
PspOMI G'GGCC_C
Cuts at: 0 567 759
Size: 567 192
PstI C_TGCA'G
Cuts at: 0 35 330 632 759
Size: 35 295 302 127
Fragments arranged by size:
302 295 127 35
PvuII CAG'CTG
Cuts at: 0 30 528 759
Size: 30 498 231
Fragments arranged by size:
498 231 30
SanDI GG'GwC_CC
Cuts at: 0 170 759
Size: 170 589
SbfI CC_TGCA'GG
Cuts at: 0 330 632 759
Size: 330 302 127
Fragments arranged by size:
330 302 127
SfcI C'TryA_G
Cuts at: 0 31 318 326 628 759
Size: 31 287 8 302 131
Fragments arranged by size:
302 287 131 31 8
StyI C'CwwG_G
Cuts at: 0 7 515 759
Size: 7 508 244
Fragments arranged by size:
508 244 7
TatI w'GTAC_w
Cuts at: 0 700 759
Size: 700 59
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 38 70 419 451 490 522 759
Size: 38 32 349 32 39 32 237
Fragments arranged by size:
349 237 39 38 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 654 724 759
Size: 351 303 70 35
Fragments arranged by size:
351 303 70 35
Enzymes that do cut:
AlwNI ApaI AvaI BaeI BanI BanII BbsI BclI
BglI BlpI Bme1580I BmrI BplI BpmI BsaI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspMI BstAPI BstEII BstXI Bsu36I BtgI Eco57MI EcoO109I
Hin4I MspA1I NcoI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII SanDI SbfI SfcI StyI TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BamHI BbeI BbvCI BcgI
BciVI BfrBI BglII BmgBI BmtI Bpu10I BpuEI BsaAI
BsaBI BsiEI BsiWI BsmI BspEI BspHI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstYI BstZ17I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SapI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XbaI XhoI
XmaI XmnI ZraI