(Linear) (Six-base) MAPSORT of: KIEE0571.seq  Check: 8677  from: 1  to: 564



KIEE0571.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     357     564 
   Size:        357     207
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     307     564 
   Size:        307     257
 
BanI G'GyrC_C

Cuts at:      0     101     564 
   Size:        101     463
 
BanII G_rGCy'C

Cuts at:      0      84     564 
   Size:         84     480
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     465     564 
   Size:        465      99
 
BglI GCCn_nnn'nGGC

Cuts at:      0     514     564 
   Size:        514      50
 
Bme1580I G_kGCm'C

Cuts at:      0     519     564 
   Size:        519      45
 
BmgBI CAC'GTC

Cuts at:      0     255     564 
   Size:        255     309
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     319     564 
   Size:         95     224     245

  Fragments arranged by size: 

                 245     224      95
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     145     534     564 
   Size:        145     389      30

  Fragments arranged by size: 

                 389     145      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      92     564 
   Size:         92     472
 
BsiHKAI G_wGCw'C

Cuts at:      0      30     564 
   Size:         30     534
 
Bsp1286I G_dGCh'C

Cuts at:      0      30      84     519     564 
   Size:         30      54     435      45

  Fragments arranged by size: 

                 435      54      45      30
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     357     564 
   Size:        357     207
 
BsrDI GCAATG_nn'

Cuts at:      0     395     564 
   Size:        395     169
 
BssSI C'ACGA_G

Cuts at:      0     388     564 
   Size:        388     176
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     108     564 
   Size:        108     456
 
BstEII G'GTnAC_C

Cuts at:      0     336     564 
   Size:        336     228
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     468     564 
   Size:        468      96
 
BtsI GCAGTG_nn'

Cuts at:      0     436     564 
   Size:        436     128
 
DraIII CAC_nnn'GTG

Cuts at:      0     108     564 
   Size:        108     456
 
EarI CTCTTCn'nnn_

Cuts at:      0     223     564 
   Size:        223     341
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      82     253     564 
   Size:         82     171     311

  Fragments arranged by size: 

                 311     171      82
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      95     307     319     564 
   Size:         95     212      12     245

  Fragments arranged by size: 

                 245     212      95      12
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     415     564 
   Size:        415     149
 
EcoO109I rG'GnC_Cy

Cuts at:      0     545     564 
   Size:        545      19
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     127     143     159     175     564 
   Size:        127      16      16      16     389

  Fragments arranged by size: 

                 389     127      16      16      16
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     206     296     564 
   Size:        206      90     268

  Fragments arranged by size: 

                 268     206      90
 
MspA1I CmG'CkG

Cuts at:      0      21     564 
   Size:         21     543
 
NspI r_CATG'y

Cuts at:      0     249     564 
   Size:        249     315
 
PstI C_TGCA'G

Cuts at:      0     368     564 
   Size:        368     196
 
PvuII CAG'CTG

Cuts at:      0      21     564 
   Size:         21     543
 
SapI GCTCTTCn'nnn_

Cuts at:      0     223     564 
   Size:        223     341
 
SbfI CC_TGCA'GG

Cuts at:      0     368     564 
   Size:        368     196
 
SfcI C'TryA_G

Cuts at:      0     364     564 
   Size:        364     200
 
SmlI C'TyrA_G

Cuts at:      0     124     549     564 
   Size:        124     425      15

  Fragments arranged by size: 

                 425     124      15
 
SphI G_CATG'C

Cuts at:      0     249     564 
   Size:        249     315
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     231     564 
   Size:        231     333
 

 Enzymes that do cut:

     AarI     AcuI     BanI    BanII     BbsI     BglI Bme1580I    BmgBI
     BpmI    BpuEI    BseRI  BsiHKAI Bsp1286I    BspMI    BsrDI    BssSI
   BstAPI   BstEII    BstXI     BtsI   DraIII     EarI     EciI  Eco57MI
    EcoNI EcoO109I    Hin4I     MmeI   MspA1I     NspI     PstI    PvuII
     SapI     SbfI     SfcI     SmlI     SphI     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI    BglII     BlpI     BmrI
     BmtI     BplI   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsaXI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BsrBI    BsrFI    BsrGI   BssHII    BstBI    BstYI  BstZ17I
   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DraI     DrdI     EaeI
     EagI  EcoICRI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI
     SrfI     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI
  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI