(Linear) (Six-base) MAPSORT of: KIEE0570.seq  Check: 4643  from: 1  to: 738



KIEE0570.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     413     738 
   Size:        413     325
 
AleI CACnn'nnGTG

Cuts at:      0     144     404     738 
   Size:        144     260     334

  Fragments arranged by size: 

                 334     260     144
 
AvaI C'yCGr_G

Cuts at:      0      68     738 
   Size:         68     670
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     268     738 
   Size:        268     470
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     652     738 
   Size:        652      86
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     509     634     738 
   Size:        349     160     125     104

  Fragments arranged by size: 

                 349     160     125     104
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601     738 
   Size:        601     137
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     691     738 
   Size:        691      47
 
BseYI C'CCAG_C

Cuts at:      0      18     738 
   Size:         18     720
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     644     738 
   Size:        644      94
 
BsiHKAI G_wGCw'C

Cuts at:      0     633     738 
   Size:        633     105
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      74     738 
   Size:         74     664
 
Bsp1286I G_dGCh'C

Cuts at:      0     633     738 
   Size:        633     105
 
BstYI r'GATC_y

Cuts at:      0     409     738 
   Size:        409     329
 
Bsu36I CC'TnA_GG

Cuts at:      0     648     738 
   Size:        648      90
 
BtgI C'CryG_G

Cuts at:      0     139     738 
   Size:        139     599
 
BtsI GCAGTG_nn'

Cuts at:      0     411     738 
   Size:        411     327
 
ClaI AT'CG_AT

Cuts at:      0     428     738 
   Size:        428     310
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     112     738 
   Size:        112     626
 
EaeI y'GGCC_r

Cuts at:      0     533     738 
   Size:        533     205
 
EarI CTCTTCn'nnn_

Cuts at:      0     457     738 
   Size:        457     281
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     349     413     509     634     738 
   Size:        349      64      96     125     104

  Fragments arranged by size: 

                 349     125     104      96      64
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     392     738 
   Size:        392     346
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      76     108     738 
   Size:         76      32     630

  Fragments arranged by size: 

                 630      76      32
 
MscI TGG'CCA

Cuts at:      0     535     738 
   Size:        535     203
 
MslI CAynn'nnrTG

Cuts at:      0     144     404     556     738 
   Size:        144     260     152     182

  Fragments arranged by size: 

                 260     182     152     144
 
MspA1I CmG'CkG

Cuts at:      0     327     738 
   Size:        327     411
 
PasI CC'CwG_GG

Cuts at:      0     228     738 
   Size:        228     510
 
PfoI T'CCnGG_A

Cuts at:      0     486     738 
   Size:        486     252
 
PstI C_TGCA'G

Cuts at:      0     365     738 
   Size:        365     373
 
PvuII CAG'CTG

Cuts at:      0     327     738 
   Size:        327     411
 
SbfI CC_TGCA'GG

Cuts at:      0     365     738 
   Size:        365     373
 
ScaI AGT'ACT

Cuts at:      0     278     738 
   Size:        278     460
 
SfcI C'TryA_G

Cuts at:      0     361     738 
   Size:        361     377
 
SmaI CCC'GGG

Cuts at:      0      70     738 
   Size:         70     668
 
SmlI C'TyrA_G

Cuts at:      0     580     738 
   Size:        580     158
 
StyI C'CwwG_G

Cuts at:      0     344     738 
   Size:        344     394
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     357     738 
   Size:        357     381
 
TatI w'GTAC_w

Cuts at:      0     276     738 
   Size:        276     462
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     363     395     738 
   Size:        363      32     343

  Fragments arranged by size: 

                 363     343      32
 
XmaI C'CCGG_G

Cuts at:      0      68     738 
   Size:         68     670
 

 Enzymes that do cut:

     AcuI     AleI     AvaI     BmrI     BplI     BpmI    BpuEI    BseRI
    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BstYI   Bsu36I     BtgI
     BtsI     ClaI     DrdI     EaeI     EarI  Eco57MI     FalI    Hin4I
     MscI     MslI   MspA1I     PasI     PfoI     PstI    PvuII     SbfI
     ScaI     SfcI     SmaI     SmlI     StyI    TaqII     TatI     TstI
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BsaXI    BsiEI    BsiWI     BsmI    BspEI
    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI
   BstAPI    BstBI   BstEII    BstXI  BstZ17I    BtgZI    CspCI     DraI
   DraIII     EagI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV
     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     NspI     PacI     PciI
    PflMI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI