(Linear) (Six-base) MAPSORT of: KIEE0569.seq  Check: 2605  from: 1  to: 582



KIEE0569.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:16  ..

AccI GT'mk_AC

Cuts at:      0     126     582 
   Size:        126     456
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     491     582 
   Size:        256     235      91

  Fragments arranged by size: 

                 256     235      91
 
AflIII A'CryG_T

Cuts at:      0     540     582 
   Size:        540      42
 
AlwNI CAG_nnn'CTG

Cuts at:      0     156     373     582 
   Size:        156     217     209

  Fragments arranged by size: 

                 217     209     156
 
ApoI r'AATT_y

Cuts at:      0     475     582 
   Size:        475     107
 
BseYI C'CCAG_C

Cuts at:      0     373     484     582 
   Size:        373     111      98

  Fragments arranged by size: 

                 373     111      98
 
BsmI GAATG_Cn'

Cuts at:      0      62     582 
   Size:         62     520
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     144     582 
   Size:        144     438
 
BstBI TT'CG_AA

Cuts at:      0     361     582 
   Size:        361     221
 
BtsI GCAGTG_nn'

Cuts at:      0     406     582 
   Size:        406     176
 
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'

Cuts at:      0     234     269     582 
   Size:        234      35     313

  Fragments arranged by size: 

                 313     234      35
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     256     491     582 
   Size:        256     235      91

  Fragments arranged by size: 

                 256     235      91
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     481     513     582 
   Size:        481      32      69

  Fragments arranged by size: 

                 481      69      32
 
HindIII A'AGCT_T

Cuts at:      0     356     582 
   Size:        356     226
 
MfeI C'AATT_G

Cuts at:      0     221     248     582 
   Size:        221      27     334

  Fragments arranged by size: 

                 334     221      27
 
MslI CAynn'nnrTG

Cuts at:      0     263     582 
   Size:        263     319
 
NspI r_CATG'y

Cuts at:      0     314     544     582 
   Size:        314     230      38

  Fragments arranged by size: 

                 314     230      38
 
PciI A'CATG_T

Cuts at:      0     540     582 
   Size:        540      42
 
SfcI C'TryA_G

Cuts at:      0     332     582 
   Size:        332     250
 
TatI w'GTAC_w

Cuts at:      0     396     582 
   Size:        396     186
 

 Enzymes that do cut:

     AccI     AcuI   AflIII    AlwNI     ApoI    BseYI     BsmI   BstAPI
    BstBI     BtsI    CspCI  Eco57MI    Hin4I  HindIII     MfeI     MslI
     NspI     PciI     SfcI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI     BbsI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
 Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI     BsgI
    BsiEI  BsiHKAI    BsiWI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
     HpaI     KasI     KpnI     MluI     MmeI     MscI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI
     SpeI     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI