Restriction map for
pF1KE0554
(Linear) (Six-base) MAPSORT of: KIEE0554.seq Check: 3816 from: 1 to: 720
KIEE0554.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 257 720
Size: 257 463
AflIII A'CryG_T
Cuts at: 0 474 720
Size: 474 246
AleI CACnn'nnGTG
Cuts at: 0 413 720
Size: 413 307
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 100 508 720
Size: 100 408 212
Fragments arranged by size:
408 212 100
AlwNI CAG_nnn'CTG
Cuts at: 0 26 306 371 720
Size: 26 280 65 349
Fragments arranged by size:
349 280 65 26
ApaI G_GGCC'C
Cuts at: 0 228 720
Size: 228 492
AvaI C'yCGr_G
Cuts at: 0 50 341 720
Size: 50 291 379
Fragments arranged by size:
379 291 50
BanII G_rGCy'C
Cuts at: 0 228 567 720
Size: 228 339 153
Fragments arranged by size:
339 228 153
BbsI GAAGACnn'nnnn_
Cuts at: 0 368 720
Size: 368 352
BciVI GTATCCnnnnn_n'
Cuts at: 0 717 720
Size: 717 3
BglI GCCn_nnn'nGGC
Cuts at: 0 72 679 720
Size: 72 607 41
Fragments arranged by size:
607 72 41
Bme1580I G_kGCm'C
Cuts at: 0 228 720
Size: 228 492
BmgBI CAC'GTC
Cuts at: 0 475 720
Size: 475 245
BmrI ACTGGGnnnn_n'
Cuts at: 0 561 703 720
Size: 561 142 17
Fragments arranged by size:
561 142 17
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 328 349 720
Size: 113 215 21 371
Fragments arranged by size:
371 215 113 21
Bpu10I CC'TnA_GC
Cuts at: 0 566 720
Size: 566 154
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 575 605 720
Size: 575 30 115
Fragments arranged by size:
575 115 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 433 720
Size: 433 287
BseYI C'CCAG_C
Cuts at: 0 194 273 296 468 720
Size: 194 79 23 172 252
Fragments arranged by size:
252 194 172 79 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 305 720
Size: 305 415
BsiEI CG_ry'CG
Cuts at: 0 338 720
Size: 338 382
BsiHKAI G_wGCw'C
Cuts at: 0 567 720
Size: 567 153
BsmI GAATG_Cn'
Cuts at: 0 223 720
Size: 223 497
Bsp1286I G_dGCh'C
Cuts at: 0 228 567 720
Size: 228 339 153
Fragments arranged by size:
339 228 153
BspHI T'CATG_A
Cuts at: 0 213 720
Size: 213 507
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 97 257 720
Size: 97 160 463
Fragments arranged by size:
463 160 97
BsrBI CCG'CTC
Cuts at: 0 666 720
Size: 666 54
BsrDI GCAATG_nn'
Cuts at: 0 485 720
Size: 485 235
BsrFI r'CCGG_y
Cuts at: 0 333 720
Size: 333 387
BsrGI T'GTAC_A
Cuts at: 0 477 720
Size: 477 243
BstYI r'GATC_y
Cuts at: 0 200 720
Size: 200 520
Bsu36I CC'TnA_GG
Cuts at: 0 529 659 720
Size: 529 130 61
Fragments arranged by size:
529 130 61
BtsI GCAGTG_nn'
Cuts at: 0 322 684 720
Size: 322 362 36
Fragments arranged by size:
362 322 36
EaeI y'GGCC_r
Cuts at: 0 17 720
Size: 17 703
EcoICRI GAG'CTC
Cuts at: 0 565 720
Size: 565 155
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 113 328 349 720
Size: 113 215 21 371
Fragments arranged by size:
371 215 113 21
EcoO109I rG'GnC_Cy
Cuts at: 0 155 224 423 671 720
Size: 155 69 199 248 49
Fragments arranged by size:
248 199 155 69 49
HaeII r_GCGC'y
Cuts at: 0 67 720
Size: 67 653
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 28 60 192 224 625 657 720
Size: 28 32 132 32 401 32 63
Fragments arranged by size:
401 132 63 32 32 32 28
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 327 720
Size: 327 393
MscI TGG'CCA
Cuts at: 0 19 720
Size: 19 701
MslI CAynn'nnrTG
Cuts at: 0 212 413 554 720
Size: 212 201 141 166
Fragments arranged by size:
212 201 166 141
MspA1I CmG'CkG
Cuts at: 0 371 413 720
Size: 371 42 307
Fragments arranged by size:
371 307 42
PflMI CCAn_nnn'nTGG
Cuts at: 0 386 720
Size: 386 334
PfoI T'CCnGG_A
Cuts at: 0 709 720
Size: 709 11
PpuMI rG'GwC_Cy
Cuts at: 0 155 720
Size: 155 565
PspOMI G'GGCC_C
Cuts at: 0 224 720
Size: 224 496
PstI C_TGCA'G
Cuts at: 0 515 720
Size: 515 205
SacI G_AGCT'C
Cuts at: 0 567 720
Size: 567 153
SanDI GG'GwC_CC
Cuts at: 0 155 720
Size: 155 565
SfcI C'TryA_G
Cuts at: 0 511 720
Size: 511 209
SmaI CCC'GGG
Cuts at: 0 52 343 720
Size: 52 291 377
Fragments arranged by size:
377 291 52
StuI AGG'CCT
Cuts at: 0 264 720
Size: 264 456
StyI C'CwwG_G
Cuts at: 0 228 677 720
Size: 228 449 43
Fragments arranged by size:
449 228 43
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 299 310 720
Size: 299 11 410
Fragments arranged by size:
410 299 11
TatI w'GTAC_w
Cuts at: 0 477 593 720
Size: 477 116 127
Fragments arranged by size:
477 127 116
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 575 607 720
Size: 575 32 113
Fragments arranged by size:
575 113 32
XmaI C'CCGG_G
Cuts at: 0 50 341 720
Size: 50 291 379
Fragments arranged by size:
379 291 50
Enzymes that do cut:
AarI AflIII AleI AlfI AlwNI ApaI AvaI BanII
BbsI BciVI BglI Bme1580I BmgBI BmrI BpmI Bpu10I
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstYI Bsu36I
BtsI EaeI EcoICRI Eco57MI EcoO109I HaeII Hin4I MmeI
MscI MslI MspA1I PflMI PfoI PpuMI PspOMI PstI
SacI SanDI SfcI SmaI StuI StyI TaqII TatI
TstI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BclI BfrBI
BglII BlpI BmtI BplI BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsiWI BsmBI BspEI BssHII BssSI BstAPI
BstBI BstEII BstXI BstZ17I BtgI BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EciI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI
PvuI PvuII RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI SwaI Tth111I XbaI XcmI XhoI
XmnI ZraI