(Linear) (Six-base) MAPSORT of: KIEE0548.seq  Check: 3595  from: 1  to: 498



KIEE0548.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AleI CACnn'nnGTG

Cuts at:      0     264     498 
   Size:        264     234
 
BanI G'GyrC_C

Cuts at:      0     396     498 
   Size:        396     102
 
BanII G_rGCy'C

Cuts at:      0      93     498 
   Size:         93     405
 
BlpI GC'TnA_GC

Cuts at:      0       5     498 
   Size:          5     493
 
Bme1580I G_kGCm'C

Cuts at:      0     401     498 
   Size:        401      97
 
BmgBI CAC'GTC

Cuts at:      0     429     498 
   Size:        429      69
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     498 
   Size:         30     468
 
BsaBI GATnn'nnATC

Cuts at:      0     461     498 
   Size:        461      37
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      36     498 
   Size:         36     462
 
BseYI C'CCAG_C

Cuts at:      0     141     498 
   Size:        141     357
 
Bsp1286I G_dGCh'C

Cuts at:      0      93     401     498 
   Size:         93     308      97

  Fragments arranged by size: 

                 308      97      93
 
BssSI C'ACGA_G

Cuts at:      0     162     498 
   Size:        162     336
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     408     498 
   Size:        408      90
 
BstYI r'GATC_y

Cuts at:      0     167     281     498 
   Size:        167     114     217

  Fragments arranged by size: 

                 217     167     114
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     220     498 
   Size:        220     278
 
EaeI y'GGCC_r

Cuts at:      0      70     311     338     410     498 
   Size:         70     241      27      72      88

  Fragments arranged by size: 

                 241      88      72      70      27
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      30     498 
   Size:         30     468
 
HincII GTy'rAC

Cuts at:      0     225     498 
   Size:        225     273
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     498 
   Size:        449      49
 
MscI TGG'CCA

Cuts at:      0      72     313     340     412     498 
   Size:         72     241      27      72      86

  Fragments arranged by size: 

                 241      86      72      72      27
 
MslI CAynn'nnrTG

Cuts at:      0     264     498 
   Size:        264     234
 
MspA1I CmG'CkG

Cuts at:      0     394     498 
   Size:        394     104
 
PfoI T'CCnGG_A

Cuts at:      0      46     191     498 
   Size:         46     145     307

  Fragments arranged by size: 

                 307     145      46
 
PstI C_TGCA'G

Cuts at:      0      98     498 
   Size:         98     400
 
SfcI C'TryA_G

Cuts at:      0      94     498 
   Size:         94     404
 
StuI AGG'CCT

Cuts at:      0     475     498 
   Size:        475      23
 
StyI C'CwwG_G

Cuts at:      0     341     498 
   Size:        341     157
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     150     230     498 
   Size:        150      80     268

  Fragments arranged by size: 

                 268     150      80
 

 Enzymes that do cut:

     AleI     BanI    BanII     BlpI Bme1580I    BmgBI     BpmI    BsaBI
    BseRI    BseYI Bsp1286I    BssSI    BstXI    BstYI     DrdI     EaeI
  Eco57MI   HincII     MmeI     MscI     MslI   MspA1I     PfoI     PstI
     SfcI     StuI     StyI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BmrI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI
    BsaHI    BsaWI    BsaXI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI
    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII   BstAPI    BstBI   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI
     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII    Hin4I  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     SwaI     TatI
     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI