Restriction map for
pF1KE0537
(Linear) (Six-base) MAPSORT of: KIEE0537.seq Check: 5869 from: 1 to: 786
KIEE0537.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AatII G_ACGT'C
Cuts at: 0 572 786
Size: 572 214
AfeI AGC'GCT
Cuts at: 0 595 786
Size: 595 191
AvaI C'yCGr_G
Cuts at: 0 545 577 786
Size: 545 32 209
Fragments arranged by size:
545 209 32
BbsI GAAGACnn'nnnn_
Cuts at: 0 564 786
Size: 564 222
BbvCI CC'TCA_GC
Cuts at: 0 337 535 786
Size: 337 198 251
Fragments arranged by size:
337 251 198
BclI T'GATC_A
Cuts at: 0 323 557 786
Size: 323 234 229
Fragments arranged by size:
323 234 229
BglII A'GATC_T
Cuts at: 0 683 786
Size: 683 103
BlpI GC'TnA_GC
Cuts at: 0 179 786
Size: 179 607
BmgBI CAC'GTC
Cuts at: 0 57 786
Size: 57 729
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 560 786
Size: 560 226
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 786
Size: 460 326
Bpu10I CC'TnA_GC
Cuts at: 0 337 355 535 786
Size: 337 18 180 251
Fragments arranged by size:
337 251 180 18
BsaHI Gr'CG_yC
Cuts at: 0 569 786
Size: 569 217
BsaWI w'CCGG_w
Cuts at: 0 392 786
Size: 392 394
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 319 457 563 716 786
Size: 319 138 106 153 70
Fragments arranged by size:
319 153 138 106 70
BseYI C'CCAG_C
Cuts at: 0 186 584 786
Size: 186 398 202
Fragments arranged by size:
398 202 186
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 268 417 760 786
Size: 157 111 149 343 26
Fragments arranged by size:
343 157 149 111 26
BsiEI CG_ry'CG
Cuts at: 0 426 786
Size: 426 360
BspEI T'CCGG_A
Cuts at: 0 392 786
Size: 392 394
BspHI T'CATG_A
Cuts at: 0 777 786
Size: 777 9
BsrFI r'CCGG_y
Cuts at: 0 75 786
Size: 75 711
BstXI CCAn_nnnn'nTGG
Cuts at: 0 646 786
Size: 646 140
BstYI r'GATC_y
Cuts at: 0 482 683 786
Size: 482 201 103
Fragments arranged by size:
482 201 103
Bsu36I CC'TnA_GG
Cuts at: 0 98 728 786
Size: 98 630 58
Fragments arranged by size:
630 98 58
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 759 786
Size: 759 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 354 389 786
Size: 354 35 397
Fragments arranged by size:
397 354 35
DraIII CAC_nnn'GTG
Cuts at: 0 717 786
Size: 717 69
EaeI y'GGCC_r
Cuts at: 0 104 423 708 786
Size: 104 319 285 78
Fragments arranged by size:
319 285 104 78
EagI C'GGCC_G
Cuts at: 0 423 786
Size: 423 363
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 460 786
Size: 460 326
EcoNI CCTnn'n_nnAGG
Cuts at: 0 301 616 786
Size: 301 315 170
Fragments arranged by size:
315 301 170
EcoO109I rG'GnC_Cy
Cuts at: 0 65 786
Size: 65 721
HaeII r_GCGC'y
Cuts at: 0 597 786
Size: 597 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 125 157 254 286 542 574 786
Size: 125 32 97 32 256 32 212
Fragments arranged by size:
256 212 125 97 32 32 32
MscI TGG'CCA
Cuts at: 0 710 786
Size: 710 76
MspA1I CmG'CkG
Cuts at: 0 186 786
Size: 186 600
NaeI GCC'GGC
Cuts at: 0 77 786
Size: 77 709
NgoMIV G'CCGG_C
Cuts at: 0 75 786
Size: 75 711
NspI r_CATG'y
Cuts at: 0 405 786
Size: 405 381
PflMI CCAn_nnn'nTGG
Cuts at: 0 717 786
Size: 717 69
PfoI T'CCnGG_A
Cuts at: 0 485 786
Size: 485 301
PpuMI rG'GwC_Cy
Cuts at: 0 65 786
Size: 65 721
PstI C_TGCA'G
Cuts at: 0 119 317 786
Size: 119 198 469
Fragments arranged by size:
469 198 119
PvuII CAG'CTG
Cuts at: 0 186 786
Size: 186 600
SanDI GG'GwC_CC
Cuts at: 0 65 786
Size: 65 721
SfcI C'TryA_G
Cuts at: 0 115 313 786
Size: 115 198 473
Fragments arranged by size:
473 198 115
SmlI C'TyrA_G
Cuts at: 0 545 786
Size: 545 241
SphI G_CATG'C
Cuts at: 0 405 786
Size: 405 381
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 178 786
Size: 178 608
XhoI C'TCGA_G
Cuts at: 0 545 786
Size: 545 241
ZraI GAC'GTC
Cuts at: 0 570 786
Size: 570 216
Enzymes that do cut:
AatII AfeI AvaI BbsI BbvCI BclI BglII BlpI
BmgBI BplI BpmI Bpu10I BsaHI BsaWI BseRI BseYI
BsgI BsiEI BspEI BspHI BsrFI BstXI BstYI Bsu36I
BtgZI CspCI DraIII EaeI EagI Eco57MI EcoNI EcoO109I
HaeII Hin4I MscI MspA1I NaeI NgoMIV NspI PflMI
PfoI PpuMI PstI PvuII SanDI SfcI SmlI SphI
TaqII XhoI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BanI BanII
BbeI BcgI BciVI BfrBI BglI Bme1580I BmrI BmtI
BpuEI BsaI BsaAI BsaBI BsaXI BsiHKAI BsiWI BsmI
BsmBI Bsp1286I BspMI BsrBI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I BtgI BtsI ClaI DraI
DrdI EarI EciI EcoICRI EcoRI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NarI NcoI NdeI NheI NotI
NruI NsiI PacI PasI PciI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SrfI SspI StuI
StyI SwaI TatI TstI Tth111I XbaI XcmI XmaI
XmnI