Restriction map for
pF1KE0535
(Linear) (Six-base) MAPSORT of: KIEE0535.seq Check: 7595 from: 1 to: 1227
KIEE0535.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 585 1227
Size: 585 642
AatII G_ACGT'C
Cuts at: 0 10 1227
Size: 10 1217
AccI GT'mk_AC
Cuts at: 0 416 1227
Size: 416 811
Acc65I G'GTAC_C
Cuts at: 0 236 1227
Size: 236 991
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 538 1038 1227
Size: 538 500 189
Fragments arranged by size:
538 500 189
AleI CACnn'nnGTG
Cuts at: 0 973 1138 1227
Size: 973 165 89
Fragments arranged by size:
973 165 89
ApaI G_GGCC'C
Cuts at: 0 661 1227
Size: 661 566
ApaLI G'TGCA_C
Cuts at: 0 669 1227
Size: 669 558
AvaI C'yCGr_G
Cuts at: 0 734 1227
Size: 734 493
BamHI G'GATC_C
Cuts at: 0 578 1227
Size: 578 649
BanI G'GyrC_C
Cuts at: 0 17 236 830 1227
Size: 17 219 594 397
Fragments arranged by size:
594 397 219 17
BanII G_rGCy'C
Cuts at: 0 661 752 893 982 1042 1132 1222 1227
Size: 661 91 141 89 60 90 90 5
Fragments arranged by size:
661 141 91 90 90 89 60 5
BbeI G_GCGC'C
Cuts at: 0 834 1227
Size: 834 393
BbvCI CC'TCA_GC
Cuts at: 0 493 1227
Size: 493 734
BglI GCCn_nnn'nGGC
Cuts at: 0 399 1227
Size: 399 828
Bme1580I G_kGCm'C
Cuts at: 0 20 661 673 1227
Size: 20 641 12 554
Fragments arranged by size:
641 554 20 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 324 1227
Size: 324 903
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 664 1227
Size: 292 372 563
Fragments arranged by size:
563 372 292
Bpu10I CC'TnA_GC
Cuts at: 0 91 493 1227
Size: 91 402 734
Fragments arranged by size:
734 402 91
BsaHI Gr'CG_yC
Cuts at: 0 7 831 1085 1227
Size: 7 824 254 142
Fragments arranged by size:
824 254 142 7
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 964 994 1114 1144 1227
Size: 964 30 120 30 83
Fragments arranged by size:
964 120 83 30 30
BseYI C'CCAG_C
Cuts at: 0 279 1227
Size: 279 948
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 22 532 994 1227
Size: 22 510 462 233
Fragments arranged by size:
510 462 233 22
BsiHKAI G_wGCw'C
Cuts at: 0 673 752 893 1109 1145 1227
Size: 673 79 141 216 36 82
Fragments arranged by size:
673 216 141 82 79 36
Bsp1286I G_dGCh'C
Cuts at: 0 20 661 673 752 893 982 1042 1109
Size: 20 641 12 79 141 89 60 67
Cuts at: 1109 1132 1145 1222 1227
Size: 23 13 77 5
Fragments arranged by size:
641 141 89 79 77 67 60 23
20 13 12 5
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 482 585 609 689 1016 1227
Size: 482 103 24 80 327 211
Fragments arranged by size:
482 327 211 103 80 24
BsrBI CCG'CTC
Cuts at: 0 909 1227
Size: 909 318
BsrFI r'CCGG_y
Cuts at: 0 239 827 1227
Size: 239 588 400
Fragments arranged by size:
588 400 239
BsrGI T'GTAC_A
Cuts at: 0 329 425 1227
Size: 329 96 802
Fragments arranged by size:
802 329 96
BstEII G'GTnAC_C
Cuts at: 0 630 729 1227
Size: 630 99 498
Fragments arranged by size:
630 498 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 210 975 1227
Size: 210 765 252
Fragments arranged by size:
765 252 210
BstYI r'GATC_y
Cuts at: 0 578 1227
Size: 578 649
BtgI C'CryG_G
Cuts at: 0 11 52 1227
Size: 11 41 1175
Fragments arranged by size:
1175 41 11
BtsI GCAGTG_nn'
Cuts at: 0 83 161 789 1227
Size: 83 78 628 438
Fragments arranged by size:
628 438 83 78
DraIII CAC_nnn'GTG
Cuts at: 0 555 1227
Size: 555 672
EarI CTCTTCn'nnn_
Cuts at: 0 898 1227
Size: 898 329
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 72 1227
Size: 72 1155
EcoICRI GAG'CTC
Cuts at: 0 750 891 1227
Size: 750 141 336
Fragments arranged by size:
750 336 141
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 292 538 664 1038 1227
Size: 292 246 126 374 189
Fragments arranged by size:
374 292 246 189 126
EcoNI CCTnn'n_nnAGG
Cuts at: 0 379 1227
Size: 379 848
EcoO109I rG'GnC_Cy
Cuts at: 0 471 678 1005 1155 1227
Size: 471 207 327 150 72
Fragments arranged by size:
471 327 207 150 72
FspI TGC'GCA
Cuts at: 0 406 1227
Size: 406 821
HaeII r_GCGC'y
Cuts at: 0 834 1227
Size: 834 393
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1070 1102 1181 1213 1227
Size: 1070 32 79 32 14
Fragments arranged by size:
1070 79 32 32 14
KasI G'GCGC_C
Cuts at: 0 830 1227
Size: 830 397
KpnI G_GTAC'C
Cuts at: 0 240 1227
Size: 240 987
MslI CAynn'nnrTG
Cuts at: 0 74 973 1138 1227
Size: 74 899 165 89
Fragments arranged by size:
899 165 89 74
MspA1I CmG'CkG
Cuts at: 0 463 726 958 1179 1227
Size: 463 263 232 221 48
Fragments arranged by size:
463 263 232 221 48
NarI GG'CG_CC
Cuts at: 0 831 1227
Size: 831 396
PasI CC'CwG_GG
Cuts at: 0 778 1215 1227
Size: 778 437 12
Fragments arranged by size:
778 437 12
PflMI CCAn_nnn'nTGG
Cuts at: 0 571 812 1227
Size: 571 241 415
Fragments arranged by size:
571 415 241
PpuMI rG'GwC_Cy
Cuts at: 0 471 678 1005 1227
Size: 471 207 327 222
Fragments arranged by size:
471 327 222 207
PspOMI G'GGCC_C
Cuts at: 0 657 1227
Size: 657 570
PvuII CAG'CTG
Cuts at: 0 726 958 1179 1227
Size: 726 232 221 48
Fragments arranged by size:
726 232 221 48
SacI G_AGCT'C
Cuts at: 0 752 893 1227
Size: 752 141 334
Fragments arranged by size:
752 334 141
SapI GCTCTTCn'nnn_
Cuts at: 0 898 1227
Size: 898 329
SfcI C'TryA_G
Cuts at: 0 103 417 1227
Size: 103 314 810
Fragments arranged by size:
810 314 103
SfoI GGC'GCC
Cuts at: 0 832 1227
Size: 832 395
SgrAI Cr'CCGG_yG
Cuts at: 0 827 1227
Size: 827 400
SmaI CCC'GGG
Cuts at: 0 736 1227
Size: 736 491
StyI C'CwwG_G
Cuts at: 0 1024 1160 1227
Size: 1024 136 67
Fragments arranged by size:
1024 136 67
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 312 1227
Size: 312 915
TatI w'GTAC_w
Cuts at: 0 254 329 425 1227
Size: 254 75 96 802
Fragments arranged by size:
802 254 96 75
Tth111I GACn'n_nGTC
Cuts at: 0 819 1227
Size: 819 408
XmaI C'CCGG_G
Cuts at: 0 734 1227
Size: 734 493
ZraI GAC'GTC
Cuts at: 0 8 1227
Size: 8 1219
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AleI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbvCI BglI Bme1580I
BmrI BpmI Bpu10I BsaHI BsaXI BseYI BsgI BsiHKAI
Bsp1286I BspMI BsrBI BsrFI BsrGI BstEII BstXI BstYI
BtgI BtsI DraIII EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I KasI KpnI MslI MspA1I
NarI PasI PflMI PpuMI PspOMI PvuII SacI SapI
SfcI SfoI SgrAI SmaI StyI TaqII TatI Tth111I
XmaI ZraI
Enzymes that do not cut:
AclI AfeI AflII AflIII AgeI AhdI AlfI AloI
AlwNI ApoI AscI AseI AsiSI AvrII BaeI BbsI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrDI BssHII BssSI
BstAPI BstBI BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EaeI EagI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI MfeI MluI MmeI MscI NaeI
NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PstI PvuI RsrII SacII SalI SanDI
SbfI ScaI SexAI SfiI SgfI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TstI XbaI XcmI
XhoI XmnI