(Linear) (Six-base) MAPSORT of: KIEE0530.seq  Check: 69  from: 1  to: 462



KIEE0530.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     169     462 
   Size:        169     293
 
AflII C'TTAA_G

Cuts at:      0     229     462 
   Size:        229     233
 
AlwNI CAG_nnn'CTG

Cuts at:      0     345     462 
   Size:        345     117
 
AvaI C'yCGr_G

Cuts at:      0     361     462 
   Size:        361     101
 
BanI G'GyrC_C

Cuts at:      0     196     462 
   Size:        196     266
 
BanII G_rGCy'C

Cuts at:      0       8     462 
   Size:          8     454
 
BfrBI ATG'CAT

Cuts at:      0     332     462 
   Size:        332     130
 
BglI GCCn_nnn'nGGC

Cuts at:      0     388     462 
   Size:        388      74
 
BlpI GC'TnA_GC

Cuts at:      0       7     462 
   Size:          7     455
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     339     462 
   Size:        339     123
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     334     427     462 
   Size:        142     192      93      35

  Fragments arranged by size: 

                 192     142      93      35
 
BsaWI w'CCGG_w

Cuts at:      0     416     462 
   Size:        416      46
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     415     445     462 
   Size:        415      30      17

  Fragments arranged by size: 

                 415      30      17
 
BseYI C'CCAG_C

Cuts at:      0     447     462 
   Size:        447      15
 
BsiHKAI G_wGCw'C

Cuts at:      0     209     462 
   Size:        209     253
 
BsmI GAATG_Cn'

Cuts at:      0     334     462 
   Size:        334     128
 
Bsp1286I G_dGCh'C

Cuts at:      0       8     209     462 
   Size:          8     201     253

  Fragments arranged by size: 

                 253     201       8
 
BspEI T'CCGG_A

Cuts at:      0     416     462 
   Size:        416      46
 
BspHI T'CATG_A

Cuts at:      0     246     462 
   Size:        246     216
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      30     462 
   Size:         30     432
 
BstEII G'GTnAC_C

Cuts at:      0     106     462 
   Size:        106     356
 
BstYI r'GATC_y

Cuts at:      0     296     462 
   Size:        296     166
 
BtgI C'CryG_G

Cuts at:      0      72     462 
   Size:         72     390
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     142     169     334     427     462 
   Size:        142      27     165      93      35

  Fragments arranged by size: 

                 165     142      93      35      27
 
EcoO109I rG'GnC_Cy

Cuts at:      0     402     462 
   Size:        402      60
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     342     374     415     447     462 
   Size:        342      32      41      32      15

  Fragments arranged by size: 

                 342      41      32      32      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     458     462 
   Size:        458       4
 
NcoI C'CATG_G

Cuts at:      0      72     462 
   Size:         72     390
 
NsiI A_TGCA'T

Cuts at:      0     334     462 
   Size:        334     128
 
NspI r_CATG'y

Cuts at:      0     283     462 
   Size:        283     179
 
PasI CC'CwG_GG

Cuts at:      0     216     384     462 
   Size:        216     168      78

  Fragments arranged by size: 

                 216     168      78
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      73     462 
   Size:         73     389
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0     301     333     462 
   Size:        301      32     129

  Fragments arranged by size: 

                 301     129      32
 
PstI C_TGCA'G

Cuts at:      0     350     462 
   Size:        350     112
 
SfcI C'TryA_G

Cuts at:      0     346     462 
   Size:        346     116
 
SmaI CCC'GGG

Cuts at:      0     363     462 
   Size:        363      99
 
SmlI C'TyrA_G

Cuts at:      0     229     462 
   Size:        229     233
 
SphI G_CATG'C

Cuts at:      0     283     462 
   Size:        283     179
 
StyI C'CwwG_G

Cuts at:      0      72     462 
   Size:         72     390
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     125     208     462 
   Size:        125      83     254

  Fragments arranged by size: 

                 254     125      83
 
XmaI C'CCGG_G

Cuts at:      0     361     462 
   Size:        361     101
 

 Enzymes that do cut:

     AcuI    AflII    AlwNI     AvaI     BanI    BanII    BfrBI     BglI
     BlpI     BplI     BpmI    BsaWI    BsaXI    BseYI  BsiHKAI     BsmI
 Bsp1286I    BspEI    BspHI   BstAPI   BstEII    BstYI     BtgI  Eco57MI
 EcoO109I    Hin4I     MmeI     NcoI     NsiI     NspI     PasI    PflMI
     PsrI     PstI     SfcI     SmaI     SmlI     SphI     StyI    TaqII
     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BglII Bme1580I    BmgBI     BmrI     BmtI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BseRI     BsgI
    BsiEI    BsiWI    BsmBI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI    BstBI    BstXI  BstZ17I   Bsu36I    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MscI     MslI   MspA1I     NaeI     NarI     NdeI   NgoMIV
     NheI     NotI     NruI     PacI     PciI     PfoI     PmeI     PmlI
     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfiI     SfoI     SgfI    SgrAI    SnaBI     SpeI     SrfI
     SspI     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XcmI
     XhoI     XmnI     ZraI