Restriction map for
pF1KE0527
(Linear) (Six-base) MAPSORT of: KIEE0527.seq Check: 7640 from: 1 to: 732
KIEE0527.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 471 732
Size: 471 261
AccI GT'mk_AC
Cuts at: 0 50 333 732
Size: 50 283 399
Fragments arranged by size:
399 283 50
AhdI GACnn_n'nnGTC
Cuts at: 0 27 732
Size: 27 705
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 290 732
Size: 290 442
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 430 462 732
Size: 430 32 270
Fragments arranged by size:
430 270 32
AlwNI CAG_nnn'CTG
Cuts at: 0 426 732
Size: 426 306
AvaI C'yCGr_G
Cuts at: 0 382 732
Size: 382 350
BamHI G'GATC_C
Cuts at: 0 725 732
Size: 725 7
BanI G'GyrC_C
Cuts at: 0 322 378 732
Size: 322 56 354
Fragments arranged by size:
354 322 56
BciVI GTATCCnnnnn_n'
Cuts at: 0 263 732
Size: 263 469
BglI GCCn_nnn'nGGC
Cuts at: 0 700 732
Size: 700 32
BlpI GC'TnA_GC
Cuts at: 0 608 732
Size: 608 124
Bme1580I G_kGCm'C
Cuts at: 0 279 383 732
Size: 279 104 349
Fragments arranged by size:
349 279 104
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 529 732
Size: 457 72 203
Fragments arranged by size:
457 203 72
Bpu10I CC'TnA_GC
Cuts at: 0 220 732
Size: 220 512
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 154 660 732
Size: 154 506 72
Fragments arranged by size:
506 154 72
BsaHI Gr'CG_yC
Cuts at: 0 238 732
Size: 238 494
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 430 460 732
Size: 430 30 272
Fragments arranged by size:
430 272 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 526 732
Size: 400 126 206
Fragments arranged by size:
400 206 126
BseYI C'CCAG_C
Cuts at: 0 32 37 732
Size: 32 5 695
Fragments arranged by size:
695 32 5
Bsp1286I G_dGCh'C
Cuts at: 0 279 383 732
Size: 279 104 349
Fragments arranged by size:
349 279 104
BspHI T'CATG_A
Cuts at: 0 285 732
Size: 285 447
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 272 471 732
Size: 272 199 261
Fragments arranged by size:
272 261 199
BsrDI GCAATG_nn'
Cuts at: 0 436 732
Size: 436 296
BsrFI r'CCGG_y
Cuts at: 0 525 732
Size: 525 207
BssSI C'ACGA_G
Cuts at: 0 272 732
Size: 272 460
BstBI TT'CG_AA
Cuts at: 0 714 732
Size: 714 18
BstYI r'GATC_y
Cuts at: 0 725 732
Size: 725 7
BstZ17I GTA'TAC
Cuts at: 0 334 732
Size: 334 398
BtgI C'CryG_G
Cuts at: 0 95 318 413 732
Size: 95 223 95 319
Fragments arranged by size:
319 223 95 95
EaeI y'GGCC_r
Cuts at: 0 203 474 559 659 732
Size: 203 271 85 100 73
Fragments arranged by size:
271 203 100 85 73
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 457 529 732
Size: 457 72 203
Fragments arranged by size:
457 203 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 652 732
Size: 652 80
EcoO109I rG'GnC_Cy
Cuts at: 0 423 461 732
Size: 423 38 271
Fragments arranged by size:
423 271 38
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 150 732
Size: 150 582
FspI TGC'GCA
Cuts at: 0 91 732
Size: 91 641
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 732
Size: 13 32 687
Fragments arranged by size:
687 32 13
MscI TGG'CCA
Cuts at: 0 476 732
Size: 476 256
NcoI C'CATG_G
Cuts at: 0 413 732
Size: 413 319
NspI r_CATG'y
Cuts at: 0 307 732
Size: 307 425
PflMI CCAn_nnn'nTGG
Cuts at: 0 354 367 732
Size: 354 13 365
Fragments arranged by size:
365 354 13
PfoI T'CCnGG_A
Cuts at: 0 647 732
Size: 647 85
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 430 462 732
Size: 430 32 270
Fragments arranged by size:
430 270 32
PstI C_TGCA'G
Cuts at: 0 269 732
Size: 269 463
SbfI CC_TGCA'GG
Cuts at: 0 269 732
Size: 269 463
SexAI A'CCwGG_T
Cuts at: 0 23 732
Size: 23 709
SfcI C'TryA_G
Cuts at: 0 265 732
Size: 265 467
SmlI C'TyrA_G
Cuts at: 0 169 639 732
Size: 169 470 93
Fragments arranged by size:
470 169 93
SphI G_CATG'C
Cuts at: 0 307 732
Size: 307 425
StuI AGG'CCT
Cuts at: 0 694 732
Size: 694 38
StyI C'CwwG_G
Cuts at: 0 413 732
Size: 413 319
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 208 592 732
Size: 208 384 140
Fragments arranged by size:
384 208 140
Enzymes that do cut:
AarI AccI AhdI AlfI AloI AlwNI AvaI BamHI
BanI BciVI BglI BlpI Bme1580I BpmI Bpu10I BpuEI
BsaHI BsaXI BseRI BseYI Bsp1286I BspHI BspMI BsrDI
BsrFI BssSI BstBI BstYI BstZ17I BtgI EaeI Eco57MI
EcoNI EcoO109I FalI FspI Hin4I MscI NcoI NspI
PflMI PfoI PpiI PstI SbfI SexAI SfcI SmlI
SphI StuI StyI TaqII
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AflII AflIII AgeI
AleI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BanII BbeI BbsI BbvCI BcgI BclI BfrBI
BglII BmgBI BmrI BmtI BplI BsaI BsaAI BsaBI
BsaWI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BsrBI BsrGI BssHII BstAPI BstEII BstXI Bsu36I BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EagI EarI
EciI EcoICRI EcoRI EcoRV FseI FspAI HaeII HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MslI
MspA1I NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpuMI PshAI
PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI
SacII SalI SanDI SapI ScaI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI