Restriction map for
pF1KE0524
(Linear) (Six-base) MAPSORT of: KIEE0524.seq Check: 1481 from: 1 to: 717
KIEE0524.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 24 717
Size: 24 693
AatII G_ACGT'C
Cuts at: 0 563 717
Size: 563 154
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 717
Size: 604 113
AflIII A'CryG_T
Cuts at: 0 389 717
Size: 389 328
ApaI G_GGCC'C
Cuts at: 0 197 717
Size: 197 520
AvaI C'yCGr_G
Cuts at: 0 410 717
Size: 410 307
BanI G'GyrC_C
Cuts at: 0 36 450 708 717
Size: 36 414 258 9
Fragments arranged by size:
414 258 36 9
BanII G_rGCy'C
Cuts at: 0 104 197 265 704 717
Size: 104 93 68 439 13
Fragments arranged by size:
439 104 93 68 13
BbsI GAAGACnn'nnnn_
Cuts at: 0 285 555 717
Size: 285 270 162
Fragments arranged by size:
285 270 162
BbvCI CC'TCA_GC
Cuts at: 0 106 717
Size: 106 611
BglI GCCn_nnn'nGGC
Cuts at: 0 373 680 717
Size: 373 307 37
Fragments arranged by size:
373 307 37
Bme1580I G_kGCm'C
Cuts at: 0 197 717
Size: 197 520
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 183 574 599 717
Size: 118 65 391 25 118
Fragments arranged by size:
391 118 118 65 25
Bpu10I CC'TnA_GC
Cuts at: 0 106 717
Size: 106 611
BsaAI yAC'GTr
Cuts at: 0 390 717
Size: 390 327
BsaHI Gr'CG_yC
Cuts at: 0 314 560 717
Size: 314 246 157
Fragments arranged by size:
314 246 157
BsaWI w'CCGG_w
Cuts at: 0 146 717
Size: 146 571
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 94 717
Size: 94 623
BseYI C'CCAG_C
Cuts at: 0 330 492 519 636 717
Size: 330 162 27 117 81
Fragments arranged by size:
330 162 117 81 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 112 717
Size: 112 605
BsiEI CG_ry'CG
Cuts at: 0 23 276 284 717
Size: 23 253 8 433
Fragments arranged by size:
433 253 23 8
BsiHKAI G_wGCw'C
Cuts at: 0 104 704 717
Size: 104 600 13
Fragments arranged by size:
600 104 13
Bsp1286I G_dGCh'C
Cuts at: 0 104 197 265 704 717
Size: 104 93 68 439 13
Fragments arranged by size:
439 104 93 68 13
BspEI T'CCGG_A
Cuts at: 0 146 717
Size: 146 571
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 24 524 717
Size: 24 500 193
Fragments arranged by size:
500 193 24
BsrFI r'CCGG_y
Cuts at: 0 39 717
Size: 39 678
BssSI C'ACGA_G
Cuts at: 0 508 717
Size: 508 209
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 717
Size: 510 207
BtgI C'CryG_G
Cuts at: 0 86 270 717
Size: 86 184 447
Fragments arranged by size:
447 184 86
DraIII CAC_nnn'GTG
Cuts at: 0 141 717
Size: 141 576
EaeI y'GGCC_r
Cuts at: 0 20 273 281 374 717
Size: 20 253 8 93 343
Fragments arranged by size:
343 253 93 20 8
EagI C'GGCC_G
Cuts at: 0 20 273 281 717
Size: 20 253 8 436
Fragments arranged by size:
436 253 20 8
EarI CTCTTCn'nnn_
Cuts at: 0 190 232 251 310 717
Size: 190 42 19 59 407
Fragments arranged by size:
407 190 59 42 19
EcoICRI GAG'CTC
Cuts at: 0 102 702 717
Size: 102 600 15
Fragments arranged by size:
600 102 15
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 118 183 574 599 604 717
Size: 118 65 391 25 5 113
Fragments arranged by size:
391 118 113 65 25 5
EcoNI CCTnn'n_nnAGG
Cuts at: 0 361 717
Size: 361 356
EcoO109I rG'GnC_Cy
Cuts at: 0 194 366 633 717
Size: 194 172 267 84
Fragments arranged by size:
267 194 172 84
HaeII r_GCGC'y
Cuts at: 0 472 717
Size: 472 245
HincII GTy'rAC
Cuts at: 0 423 717
Size: 423 294
MscI TGG'CCA
Cuts at: 0 376 717
Size: 376 341
MslI CAynn'nnrTG
Cuts at: 0 376 382 508 717
Size: 376 6 126 209
Fragments arranged by size:
376 209 126 6
MspA1I CmG'CkG
Cuts at: 0 88 96 272 640 717
Size: 88 8 176 368 77
Fragments arranged by size:
368 176 88 77 8
NaeI GCC'GGC
Cuts at: 0 41 717
Size: 41 676
NgoMIV G'CCGG_C
Cuts at: 0 39 717
Size: 39 678
NotI GC'GGCC_GC
Cuts at: 0 273 717
Size: 273 444
NspI r_CATG'y
Cuts at: 0 225 252 717
Size: 225 27 465
Fragments arranged by size:
465 225 27
PasI CC'CwG_GG
Cuts at: 0 198 685 717
Size: 198 487 32
Fragments arranged by size:
487 198 32
PfoI T'CCnGG_A
Cuts at: 0 551 717
Size: 551 166
PspOMI G'GGCC_C
Cuts at: 0 193 717
Size: 193 524
PstI C_TGCA'G
Cuts at: 0 35 599 717
Size: 35 564 118
Fragments arranged by size:
564 118 35
PvuII CAG'CTG
Cuts at: 0 96 640 717
Size: 96 544 77
Fragments arranged by size:
544 96 77
SacI G_AGCT'C
Cuts at: 0 104 704 717
Size: 104 600 13
Fragments arranged by size:
600 104 13
SacII CC_GC'GG
Cuts at: 0 89 273 717
Size: 89 184 444
Fragments arranged by size:
444 184 89
SapI GCTCTTCn'nnn_
Cuts at: 0 232 717
Size: 232 485
SbfI CC_TGCA'GG
Cuts at: 0 35 717
Size: 35 682
SfcI C'TryA_G
Cuts at: 0 31 595 717
Size: 31 564 122
Fragments arranged by size:
564 122 31
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 373 717
Size: 373 344
SphI G_CATG'C
Cuts at: 0 252 717
Size: 252 465
StyI C'CwwG_G
Cuts at: 0 463 717
Size: 463 254
Tth111I GACn'n_nGTC
Cuts at: 0 289 717
Size: 289 428
ZraI GAC'GTC
Cuts at: 0 561 717
Size: 561 156
Enzymes that do cut:
AarI AatII AcuI AflIII ApaI AvaI BanI BanII
BbsI BbvCI BglI Bme1580I BpmI Bpu10I BsaAI BsaHI
BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI
BspMI BsrFI BssSI BstXI BtgI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I HaeII HincII MscI
MslI MspA1I NaeI NgoMIV NotI NspI PasI PfoI
PspOMI PstI PvuII SacI SacII SapI SbfI SfcI
SfiI SphI StyI Tth111I ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI AlwNI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglII BlpI BmgBI BmrI BmtI BplI BpuEI BsaI
BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrBI BsrDI
BsrGI BssHII BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DraI DrdI EciI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HindIII HpaI
KasI KpnI MfeI MluI MmeI NarI NcoI NdeI
NheI NruI NsiI PacI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SalI SanDI ScaI SexAI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SrfI SspI StuI SwaI TaqII
TatI TstI XbaI XcmI XhoI XmaI XmnI