(Linear) (Six-base) MAPSORT of: KIEE0522.seq  Check: 5461  from: 1  to: 480



KIEE0522.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     268     480 
   Size:        268     212
 
ApoI r'AATT_y

Cuts at:      0     411     480 
   Size:        411      69
 
BmtI G_CTAG'C

Cuts at:      0     226     480 
   Size:        226     254
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     480 
   Size:        231     249
 
Bpu10I CC'TnA_GC

Cuts at:      0     179     480 
   Size:        179     301
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     217     480 
   Size:        217     263
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     276     480 
   Size:        276     204
 
BsrDI GCAATG_nn'

Cuts at:      0     330     480 
   Size:        330     150
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     268     480 
   Size:        268     212
 
BstYI r'GATC_y

Cuts at:      0     360     480 
   Size:        360     120
 
BtgI C'CryG_G

Cuts at:      0      62     480 
   Size:         62     418
 
DraI TTT'AAA

Cuts at:      0     159     480 
   Size:        159     321
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     199     480 
   Size:        199     281
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     231     480 
   Size:        231     249
 
EcoO109I rG'GnC_Cy

Cuts at:      0     265     480 
   Size:        265     215
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     211     480 
   Size:        211     269
 
NcoI C'CATG_G

Cuts at:      0      62     480 
   Size:         62     418
 
NheI G'CTAG_C

Cuts at:      0     222     480 
   Size:        222     258
 
PpuMI rG'GwC_Cy

Cuts at:      0     265     480 
   Size:        265     215
 
PstI C_TGCA'G

Cuts at:      0     221     480 
   Size:        221     259
 
SfcI C'TryA_G

Cuts at:      0     217     308     480 
   Size:        217      91     172

  Fragments arranged by size: 

                 217     172      91
 
SmlI C'TyrA_G

Cuts at:      0     196     480 
   Size:        196     284
 
StyI C'CwwG_G

Cuts at:      0      62     480 
   Size:         62     418
 
TatI w'GTAC_w

Cuts at:      0     242     480 
   Size:        242     238
 

 Enzymes that do cut:

    AlwNI     ApoI     BmtI     BpmI   Bpu10I    BpuEI    BspMI    BsrDI
   BstAPI    BstYI     BtgI     DraI     EciI  Eco57MI EcoO109I     FalI
     NcoI     NheI    PpuMI     PstI     SfcI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BplI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI   DraIII     DrdI
     EaeI     EagI     EarI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI
     FspI    FspAI    HaeII    Hin4I   HincII  HindIII     HpaI     KasI
     KpnI     MfeI     MluI     MmeI     MscI     MslI   MspA1I     NaeI
     NarI     NdeI   NgoMIV     NotI     NruI     NsiI     NspI     PacI
     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI
     XhoI     XmaI     XmnI     ZraI