Restriction map for
pF1KE0520
(Linear) (Six-base) MAPSORT of: KIEE0520.seq Check: 9060 from: 1 to: 1050
KIEE0520.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 595 1050
Size: 595 455
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 1050
Size: 469 581
AleI CACnn'nnGTG
Cuts at: 0 162 874 1050
Size: 162 712 176
Fragments arranged by size:
712 176 162
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 603 1050
Size: 603 447
AlwNI CAG_nnn'CTG
Cuts at: 0 64 686 1050
Size: 64 622 364
Fragments arranged by size:
622 364 64
ApaI G_GGCC'C
Cuts at: 0 89 141 1050
Size: 89 52 909
Fragments arranged by size:
909 89 52
ApaLI G'TGCA_C
Cuts at: 0 217 1050
Size: 217 833
AvaI C'yCGr_G
Cuts at: 0 91 140 1050
Size: 91 49 910
Fragments arranged by size:
910 91 49
BanI G'GyrC_C
Cuts at: 0 296 1050
Size: 296 754
BanII G_rGCy'C
Cuts at: 0 89 141 1050
Size: 89 52 909
Fragments arranged by size:
909 89 52
BbeI G_GCGC'C
Cuts at: 0 300 1050
Size: 300 750
BbvCI CC'TCA_GC
Cuts at: 0 42 928 1050
Size: 42 886 122
Fragments arranged by size:
886 122 42
BglI GCCn_nnn'nGGC
Cuts at: 0 145 451 1000 1050
Size: 145 306 549 50
Fragments arranged by size:
549 306 145 50
BlpI GC'TnA_GC
Cuts at: 0 778 1050
Size: 778 272
Bme1580I G_kGCm'C
Cuts at: 0 89 141 221 294 395 624 927 1050
Size: 89 52 80 73 101 229 303 123
Fragments arranged by size:
303 229 123 101 89 80 73 52
BmgBI CAC'GTC
Cuts at: 0 356 855 1050
Size: 356 499 195
Fragments arranged by size:
499 356 195
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 736 1050
Size: 557 179 314
Fragments arranged by size:
557 314 179
Bpu10I CC'TnA_GC
Cuts at: 0 42 74 928 1050
Size: 42 32 854 122
Fragments arranged by size:
854 122 42 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1050
Size: 586 464
BsaAI yAC'GTr
Cuts at: 0 936 1050
Size: 936 114
BsaHI Gr'CG_yC
Cuts at: 0 297 1050
Size: 297 753
BsaWI w'CCGG_w
Cuts at: 0 545 1050
Size: 545 505
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 649 1050
Size: 649 401
BseYI C'CCAG_C
Cuts at: 0 82 462 679 1036 1050
Size: 82 380 217 357 14
Fragments arranged by size:
380 357 217 82 14
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 238 722 1050
Size: 238 484 328
Fragments arranged by size:
484 328 238
BsiHKAI G_wGCw'C
Cuts at: 0 23 75 221 1050
Size: 23 52 146 829
Fragments arranged by size:
829 146 52 23
Bsp1286I G_dGCh'C
Cuts at: 0 23 75 89 141 221 294 395 624
Size: 23 52 14 52 80 73 101 229
Cuts at: 624 927 1050
Size: 303 123
Fragments arranged by size:
303 229 123 101 80 73 52 52
23 14
BspEI T'CCGG_A
Cuts at: 0 545 1050
Size: 545 505
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 595 1050
Size: 595 455
BsrBI CCG'CTC
Cuts at: 0 507 1050
Size: 507 543
BsrFI r'CCGG_y
Cuts at: 0 559 1050
Size: 559 491
BstAPI GCAn_nnn'nTGC
Cuts at: 0 320 1050
Size: 320 730
BstEII G'GTnAC_C
Cuts at: 0 651 1050
Size: 651 399
BstYI r'GATC_y
Cuts at: 0 206 1050
Size: 206 844
Bsu36I CC'TnA_GG
Cuts at: 0 113 382 1050
Size: 113 269 668
Fragments arranged by size:
668 269 113
BtgI C'CryG_G
Cuts at: 0 149 359 497 594 872 1050
Size: 149 210 138 97 278 178
Fragments arranged by size:
278 210 178 149 138 97
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 927 1050
Size: 927 123
BtsI GCAGTG_nn'
Cuts at: 0 740 1050
Size: 740 310
EaeI y'GGCC_r
Cuts at: 0 132 146 491 809 1050
Size: 132 14 345 318 241
Fragments arranged by size:
345 318 241 132 14
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1038 1050
Size: 1038 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 469 557 736 1050
Size: 469 88 179 314
Fragments arranged by size:
469 314 179 88
EcoNI CCTnn'n_nnAGG
Cuts at: 0 628 1050
Size: 628 422
EcoO109I rG'GnC_Cy
Cuts at: 0 86 117 1050
Size: 86 31 933
Fragments arranged by size:
933 86 31
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 225 1050
Size: 225 825
HaeII r_GCGC'y
Cuts at: 0 300 457 1050
Size: 300 157 593
Fragments arranged by size:
593 300 157
KasI G'GCGC_C
Cuts at: 0 296 1050
Size: 296 754
MscI TGG'CCA
Cuts at: 0 148 811 1050
Size: 148 663 239
Fragments arranged by size:
663 239 148
MslI CAynn'nnrTG
Cuts at: 0 26 162 262 358 874 1050
Size: 26 136 100 96 516 176
Fragments arranged by size:
516 176 136 100 96 26
MspA1I CmG'CkG
Cuts at: 0 8 64 596 782 1050
Size: 8 56 532 186 268
Fragments arranged by size:
532 268 186 56 8
NarI GG'CG_CC
Cuts at: 0 297 1050
Size: 297 753
NcoI C'CATG_G
Cuts at: 0 149 359 497 1050
Size: 149 210 138 553
Fragments arranged by size:
553 210 149 138
NspI r_CATG'y
Cuts at: 0 189 261 281 1050
Size: 189 72 20 769
Fragments arranged by size:
769 189 72 20
PflMI CCAn_nnn'nTGG
Cuts at: 0 858 1050
Size: 858 192
PshAI GACnn'nnGTC
Cuts at: 0 603 1050
Size: 603 447
PspOMI G'GGCC_C
Cuts at: 0 85 137 1050
Size: 85 52 913
Fragments arranged by size:
913 85 52
PstI C_TGCA'G
Cuts at: 0 529 632 1050
Size: 529 103 418
Fragments arranged by size:
529 418 103
PvuII CAG'CTG
Cuts at: 0 64 782 1050
Size: 64 718 268
Fragments arranged by size:
718 268 64
SacII CC_GC'GG
Cuts at: 0 597 1050
Size: 597 453
SfcI C'TryA_G
Cuts at: 0 525 628 1050
Size: 525 103 422
Fragments arranged by size:
525 422 103
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 145 1050
Size: 145 905
SfoI GGC'GCC
Cuts at: 0 298 1050
Size: 298 752
SmlI C'TyrA_G
Cuts at: 0 601 1050
Size: 601 449
SphI G_CATG'C
Cuts at: 0 281 1050
Size: 281 769
StyI C'CwwG_G
Cuts at: 0 149 359 497 1050
Size: 149 210 138 553
Fragments arranged by size:
553 210 149 138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 156 1050
Size: 156 894
Enzymes that do cut:
AarI AcuI AleI AlfI AlwNI ApaI ApaLI AvaI
BanI BanII BbeI BbvCI BglI BlpI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI
BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI BsrFI BstAPI
BstEII BstYI Bsu36I BtgI BtgZI BtsI EaeI EciI
Eco57MI EcoNI EcoO109I FalI HaeII KasI MscI MslI
MspA1I NarI NcoI NspI PflMI PshAI PspOMI PstI
PvuII SacII SfcI SfiI SfoI SmlI SphI StyI
XcmI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI ApoI AscI AseI AsiSI AvrII BaeI
BamHI BbsI BcgI BciVI BclI BfrBI BglII BmrI
BmtI BplI BsaI BsaBI BsaXI BsiEI BsiWI BsmI
BsmBI BspHI BsrDI BsrGI BssHII BssSI BstBI BstXI
BstZ17I ClaI CspCI DraI DraIII DrdI EagI EarI
EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI NaeI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PciI
PfoI PmeI PmlI PpiI PpuMI PsiI PspXI PsrI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI