Restriction map for
pF1KE0516
(Linear) (Six-base) MAPSORT of: KIEE0516.seq Check: 8240 from: 1 to: 921
KIEE0516.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
ApoI r'AATT_y
Cuts at: 0 367 612 831 921
Size: 367 245 219 90
Fragments arranged by size:
367 245 219 90
AvaI C'yCGr_G
Cuts at: 0 232 921
Size: 232 689
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 282 315 921
Size: 282 33 606
Fragments arranged by size:
606 282 33
BanII G_rGCy'C
Cuts at: 0 169 921
Size: 169 752
BbsI GAAGACnn'nnnn_
Cuts at: 0 894 921
Size: 894 27
BlpI GC'TnA_GC
Cuts at: 0 11 921
Size: 11 910
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 921
Size: 849 72
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 532 921
Size: 532 389
BsaAI yAC'GTr
Cuts at: 0 298 921
Size: 298 623
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 861 921
Size: 861 60
BsiHKAI G_wGCw'C
Cuts at: 0 169 865 921
Size: 169 696 56
Fragments arranged by size:
696 169 56
BsmI GAATG_Cn'
Cuts at: 0 811 921
Size: 811 110
Bsp1286I G_dGCh'C
Cuts at: 0 169 865 921
Size: 169 696 56
Fragments arranged by size:
696 169 56
BspHI T'CATG_A
Cuts at: 0 701 921
Size: 701 220
BsrDI GCAATG_nn'
Cuts at: 0 816 921
Size: 816 105
BstYI r'GATC_y
Cuts at: 0 324 334 921
Size: 324 10 587
Fragments arranged by size:
587 324 10
Bsu36I CC'TnA_GG
Cuts at: 0 145 921
Size: 145 776
BtgI C'CryG_G
Cuts at: 0 329 921
Size: 329 592
BtsI GCAGTG_nn'
Cuts at: 0 5 21 238 572 921
Size: 5 16 217 334 349
Fragments arranged by size:
349 334 217 16 5
DraI TTT'AAA
Cuts at: 0 203 600 921
Size: 203 397 321
Fragments arranged by size:
397 321 203
EaeI y'GGCC_r
Cuts at: 0 2 921
Size: 2 919
EarI CTCTTCn'nnn_
Cuts at: 0 868 921
Size: 868 53
EcoICRI GAG'CTC
Cuts at: 0 167 921
Size: 167 754
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 849 921
Size: 849 72
EcoO109I rG'GnC_Cy
Cuts at: 0 150 507 921
Size: 150 357 414
Fragments arranged by size:
414 357 150
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 73 105 759 791 921
Size: 73 32 654 32 130
Fragments arranged by size:
654 130 73 32 32
HindIII A'AGCT_T
Cuts at: 0 426 921
Size: 426 495
MscI TGG'CCA
Cuts at: 0 4 921
Size: 4 917
MslI CAynn'nnrTG
Cuts at: 0 188 921
Size: 188 733
NcoI C'CATG_G
Cuts at: 0 329 921
Size: 329 592
PfoI T'CCnGG_A
Cuts at: 0 865 921
Size: 865 56
PpuMI rG'GwC_Cy
Cuts at: 0 507 921
Size: 507 414
SacI G_AGCT'C
Cuts at: 0 169 921
Size: 169 752
ScaI AGT'ACT
Cuts at: 0 308 921
Size: 308 613
SmaI CCC'GGG
Cuts at: 0 234 921
Size: 234 687
SmlI C'TyrA_G
Cuts at: 0 511 921
Size: 511 410
SnaBI TAC'GTA
Cuts at: 0 298 921
Size: 298 623
StyI C'CwwG_G
Cuts at: 0 329 921
Size: 329 592
TatI w'GTAC_w
Cuts at: 0 306 421 534 921
Size: 306 115 113 387
Fragments arranged by size:
387 306 115 113
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 870 902 921
Size: 870 32 19
Fragments arranged by size:
870 32 19
XbaI T'CTAG_A
Cuts at: 0 783 921
Size: 783 138
XmaI C'CCGG_G
Cuts at: 0 232 921
Size: 232 689
XmnI GAAnn'nnTTC
Cuts at: 0 901 921
Size: 901 20
Enzymes that do cut:
ApoI AvaI BaeI BanII BbsI BlpI BpmI BpuEI
BsaAI BsgI BsiHKAI BsmI Bsp1286I BspHI BsrDI BstYI
Bsu36I BtgI BtsI DraI EaeI EarI EcoICRI Eco57MI
EcoO109I Hin4I HindIII MscI MslI NcoI PfoI PpuMI
SacI ScaI SmaI SmlI SnaBI StyI TatI TstI
XbaI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI AscI AseI AsiSI AvrII BamHI BanI BbeI
BbvCI BcgI BciVI BclI BfrBI BglI BglII Bme1580I
BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI BsaHI
BsaWI BsaXI BseRI BseYI BsiEI BsiWI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstZ17I BtgZI ClaI CspCI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HpaI KasI KpnI MfeI MluI
MmeI MspA1I NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacII SalI SanDI SapI SbfI
SexAI SfcI SfiI SfoI SgfI SgrAI SpeI SphI
SrfI SspI StuI SwaI TaqII Tth111I XcmI XhoI
ZraI