(Linear) (Six-base) MAPSORT of: KIEE0509.seq  Check: 2149  from: 1  to: 510



KIEE0509.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     103     510 
   Size:          6      97     407

  Fragments arranged by size: 

                 407      97       6
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     410     510 
   Size:        410     100
 
ApoI r'AATT_y

Cuts at:      0     235     510 
   Size:        235     275
 
BlpI GC'TnA_GC

Cuts at:      0     383     510 
   Size:        383     127
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     185     510 
   Size:        185     325
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     104     510 
   Size:        104     406
 
BsiEI CG_ry'CG

Cuts at:      0      15     510 
   Size:         15     495
 
BsrFI r'CCGG_y

Cuts at:      0      14     510 
   Size:         14     496
 
BtgI C'CryG_G

Cuts at:      0     287     510 
   Size:        287     223
 
BtsI GCAGTG_nn'

Cuts at:      0     401     510 
   Size:        401     109
 
DraIII CAC_nnn'GTG

Cuts at:      0      97     510 
   Size:         97     413
 
EaeI y'GGCC_r

Cuts at:      0      12     442     510 
   Size:         12     430      68

  Fragments arranged by size: 

                 430      68      12
 
EagI C'GGCC_G

Cuts at:      0      12     510 
   Size:         12     498
 
EarI CTCTTCn'nnn_

Cuts at:      0     223     491     510 
   Size:        223     268      19

  Fragments arranged by size: 

                 268     223      19
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0       6     103     104     510 
   Size:          6      97       1     406

  Fragments arranged by size: 

                 406      97       6       1
 
FseI GG_CCGG'CC

Cuts at:      0      18     510 
   Size:         18     492
 
HincII GTy'rAC

Cuts at:      0     180     510 
   Size:        180     330
 
HindIII A'AGCT_T

Cuts at:      0     115     510 
   Size:        115     395
 
HpaI GTT'AAC

Cuts at:      0     180     510 
   Size:        180     330
 
NaeI GCC'GGC

Cuts at:      0      16     510 
   Size:         16     494
 
NcoI C'CATG_G

Cuts at:      0     287     510 
   Size:        287     223
 
NgoMIV G'CCGG_C

Cuts at:      0      14     510 
   Size:         14     496
 
NspI r_CATG'y

Cuts at:      0     391     510 
   Size:        391     119
 
PsiI TTA'TAA

Cuts at:      0     464     510 
   Size:        464      46
 
StyI C'CwwG_G

Cuts at:      0     287     510 
   Size:        287     223
 

 Enzymes that do cut:

     AcuI     AlfI     ApoI     BlpI     BmrI     BpmI    BsiEI    BsrFI
     BtgI     BtsI   DraIII     EaeI     EagI     EarI  Eco57MI     FseI
   HincII  HindIII     HpaI     NaeI     NcoI   NgoMIV     NspI     PsiI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII Bme1580I    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI
    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
  BsiHKAI    BsiWI     BsmI    BsmBI Bsp1286I    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI
     DrdI     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI
     FspI    FspAI    HaeII    Hin4I     KasI     KpnI     MfeI     MluI
     MmeI     MscI     MslI   MspA1I     NarI     NdeI     NheI     NotI
     NruI     NsiI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI
     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI