Restriction map for
pF1KE0493
(Linear) (Six-base) MAPSORT of: KIEE0493.seq Check: 8199 from: 1 to: 1185
KIEE0493.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 278 1185
Size: 278 907
AccI GT'mk_AC
Cuts at: 0 691 1185
Size: 691 494
Acc65I G'GTAC_C
Cuts at: 0 581 1185
Size: 581 604
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 673 1185
Size: 673 512
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 550 1185
Size: 550 635
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 425 457 1185
Size: 425 32 728
Fragments arranged by size:
728 425 32
AlwNI CAG_nnn'CTG
Cuts at: 0 603 1185
Size: 603 582
ApaI G_GGCC'C
Cuts at: 0 398 1185
Size: 398 787
ApoI r'AATT_y
Cuts at: 0 1099 1185
Size: 1099 86
AscI GG'CGCG_CC
Cuts at: 0 108 1185
Size: 108 1077
AvaI C'yCGr_G
Cuts at: 0 196 293 1185
Size: 196 97 892
Fragments arranged by size:
892 196 97
BanI G'GyrC_C
Cuts at: 0 155 182 581 1185
Size: 155 27 399 604
Fragments arranged by size:
604 399 155 27
BanII G_rGCy'C
Cuts at: 0 293 398 554 1185
Size: 293 105 156 631
Fragments arranged by size:
631 293 156 105
BbeI G_GCGC'C
Cuts at: 0 186 1185
Size: 186 999
BbsI GAAGACnn'nnnn_
Cuts at: 0 405 702 1185
Size: 405 297 483
Fragments arranged by size:
483 405 297
BbvCI CC'TCA_GC
Cuts at: 0 205 232 1185
Size: 205 27 953
Fragments arranged by size:
953 205 27
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 919 953 1185
Size: 919 34 232
Fragments arranged by size:
919 232 34
BglI GCCn_nnn'nGGC
Cuts at: 0 558 1185
Size: 558 627
Bme1580I G_kGCm'C
Cuts at: 0 85 398 1185
Size: 85 313 787
Fragments arranged by size:
787 313 85
BmrI ACTGGGnnnn_n'
Cuts at: 0 1116 1185
Size: 1116 69
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 993 1185
Size: 993 192
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 1060 1185
Size: 658 402 125
Fragments arranged by size:
658 402 125
Bpu10I CC'TnA_GC
Cuts at: 0 205 232 1185
Size: 205 27 953
Fragments arranged by size:
953 205 27
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 899 1185
Size: 449 450 286
Fragments arranged by size:
450 449 286
BsaI GGTCTCn'nnnn_
Cuts at: 0 964 1185
Size: 964 221
BsaHI Gr'CG_yC
Cuts at: 0 131 183 1185
Size: 131 52 1002
Fragments arranged by size:
1002 131 52
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 214 301 1000 1167 1185
Size: 214 87 699 167 18
Fragments arranged by size:
699 214 167 87 18
BseYI C'CCAG_C
Cuts at: 0 274 390 398 1185
Size: 274 116 8 787
Fragments arranged by size:
787 274 116 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 211 1185
Size: 211 974
BsiEI CG_ry'CG
Cuts at: 0 8 388 1185
Size: 8 380 797
Fragments arranged by size:
797 380 8
Bsp1286I G_dGCh'C
Cuts at: 0 85 293 398 554 1185
Size: 85 208 105 156 631
Fragments arranged by size:
631 208 156 105 85
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 248 278 611 641 1185
Size: 248 30 333 30 544
Fragments arranged by size:
544 333 248 30 30
BsrBI CCG'CTC
Cuts at: 0 224 384 579 1185
Size: 224 160 195 606
Fragments arranged by size:
606 224 195 160
BsrDI GCAATG_nn'
Cuts at: 0 497 1185
Size: 497 688
BsrFI r'CCGG_y
Cuts at: 0 425 1185
Size: 425 760
BsrGI T'GTAC_A
Cuts at: 0 644 1185
Size: 644 541
BssHII G'CGCG_C
Cuts at: 0 108 1185
Size: 108 1077
BssSI C'ACGA_G
Cuts at: 0 868 1185
Size: 868 317
BstAPI GCAn_nnn'nTGC
Cuts at: 0 499 1185
Size: 499 686
BstXI CCAn_nnnn'nTGG
Cuts at: 0 510 1185
Size: 510 675
BstYI r'GATC_y
Cuts at: 0 593 1185
Size: 593 592
Bsu36I CC'TnA_GG
Cuts at: 0 673 1185
Size: 673 512
BtgI C'CryG_G
Cuts at: 0 572 1185
Size: 572 613
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 683 1185
Size: 683 502
EaeI y'GGCC_r
Cuts at: 0 5 23 53 451 1185
Size: 5 18 30 398 734
Fragments arranged by size:
734 398 30 18 5
EagI C'GGCC_G
Cuts at: 0 5 1185
Size: 5 1180
EarI CTCTTCn'nnn_
Cuts at: 0 250 1185
Size: 250 935
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 658 673 1060 1185
Size: 658 15 387 125
Fragments arranged by size:
658 387 125 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 952 1185
Size: 952 233
EcoO109I rG'GnC_Cy
Cuts at: 0 176 394 395 600 1185
Size: 176 218 1 205 585
Fragments arranged by size:
585 218 205 176 1
HaeII r_GCGC'y
Cuts at: 0 141 186 1185
Size: 141 45 999
Fragments arranged by size:
999 141 45
KasI G'GCGC_C
Cuts at: 0 182 1185
Size: 182 1003
KpnI G_GTAC'C
Cuts at: 0 585 1185
Size: 585 600
MscI TGG'CCA
Cuts at: 0 55 1185
Size: 55 1130
MslI CAynn'nnrTG
Cuts at: 0 508 532 784 842 1185
Size: 508 24 252 58 343
Fragments arranged by size:
508 343 252 58 24
MspA1I CmG'CkG
Cuts at: 0 19 251 402 1161 1185
Size: 19 232 151 759 24
Fragments arranged by size:
759 232 151 24 19
NaeI GCC'GGC
Cuts at: 0 427 1185
Size: 427 758
NarI GG'CG_CC
Cuts at: 0 183 1185
Size: 183 1002
NcoI C'CATG_G
Cuts at: 0 572 1185
Size: 572 613
NgoMIV G'CCGG_C
Cuts at: 0 425 1185
Size: 425 760
PfoI T'CCnGG_A
Cuts at: 0 260 596 728 1185
Size: 260 336 132 457
Fragments arranged by size:
457 336 260 132
PpuMI rG'GwC_Cy
Cuts at: 0 600 1185
Size: 600 585
PshAI GACnn'nnGTC
Cuts at: 0 1168 1185
Size: 1168 17
PsiI TTA'TAA
Cuts at: 0 809 1185
Size: 809 376
PspOMI G'GGCC_C
Cuts at: 0 394 1185
Size: 394 791
PvuII CAG'CTG
Cuts at: 0 19 402 1161 1185
Size: 19 383 759 24
Fragments arranged by size:
759 383 24 19
SapI GCTCTTCn'nnn_
Cuts at: 0 250 1185
Size: 250 935
ScaI AGT'ACT
Cuts at: 0 166 1185
Size: 166 1019
SfcI C'TryA_G
Cuts at: 0 813 1185
Size: 813 372
SfoI GGC'GCC
Cuts at: 0 184 1185
Size: 184 1001
SmaI CCC'GGG
Cuts at: 0 295 1185
Size: 295 890
SmlI C'TyrA_G
Cuts at: 0 464 914 1185
Size: 464 450 271
Fragments arranged by size:
464 450 271
StuI AGG'CCT
Cuts at: 0 230 1185
Size: 230 955
StyI C'CwwG_G
Cuts at: 0 545 572 1185
Size: 545 27 613
Fragments arranged by size:
613 545 27
TatI w'GTAC_w
Cuts at: 0 164 437 644 797 1185
Size: 164 273 207 153 388
Fragments arranged by size:
388 273 207 164 153
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1165 1185
Size: 1165 20
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 562 1185
Size: 562 623
XmaI C'CCGG_G
Cuts at: 0 293 1185
Size: 293 892
Enzymes that do cut:
AarI AccI Acc65I AcuI AlfI AloI AlwNI ApaI
ApoI AscI AvaI BanI BanII BbeI BbsI BbvCI
BcgI BglI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaI BsaHI BseRI BseYI BsgI BsiEI Bsp1286I BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstXI
BstYI Bsu36I BtgI BtgZI EaeI EagI EarI Eco57MI
EcoNI EcoO109I HaeII KasI KpnI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV PfoI PpuMI PshAI PsiI
PspOMI PvuII SapI ScaI SfcI SfoI SmaI SmlI
StuI StyI TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI AhdI AleI
ApaLI AseI AsiSI AvrII BaeI BamHI BciVI BclI
BfrBI BglII BlpI BmgBI BmtI BsaAI BsaBI BsaWI
BsaXI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BstBI
BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
Hin4I HincII HindIII HpaI MfeI MluI MmeI NdeI
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PmeI PmlI PpiI PspXI PsrI PstI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI SwaI
TaqII Tth111I XbaI XhoI XmnI ZraI