Restriction map for
pF1KE0492
(Linear) (Six-base) MAPSORT of: KIEE0492.seq Check: 778 from: 1 to: 840
KIEE0492.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 840
Size: 298 542
AfeI AGC'GCT
Cuts at: 0 171 840
Size: 171 669
AhdI GACnn_n'nnGTC
Cuts at: 0 264 840
Size: 264 576
AlwNI CAG_nnn'CTG
Cuts at: 0 135 153 840
Size: 135 18 687
Fragments arranged by size:
687 135 18
ApaI G_GGCC'C
Cuts at: 0 45 840
Size: 45 795
AvaI C'yCGr_G
Cuts at: 0 303 324 840
Size: 303 21 516
Fragments arranged by size:
516 303 21
AvrII C'CTAG_G
Cuts at: 0 186 840
Size: 186 654
BanI G'GyrC_C
Cuts at: 0 752 840
Size: 752 88
BanII G_rGCy'C
Cuts at: 0 45 292 339 381 832 840
Size: 45 247 47 42 451 8
Fragments arranged by size:
451 247 47 45 42 8
BbsI GAAGACnn'nnnn_
Cuts at: 0 504 573 736 840
Size: 504 69 163 104
Fragments arranged by size:
504 163 104 69
BbvCI CC'TCA_GC
Cuts at: 0 641 840
Size: 641 199
BlpI GC'TnA_GC
Cuts at: 0 338 840
Size: 338 502
Bme1580I G_kGCm'C
Cuts at: 0 45 840
Size: 45 795
Bpu10I CC'TnA_GC
Cuts at: 0 380 641 840
Size: 380 261 199
Fragments arranged by size:
380 261 199
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 840
Size: 158 682
BsaI GGTCTCn'nnnn_
Cuts at: 0 480 840
Size: 480 360
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 291 405 496 564 658 840
Size: 291 114 91 68 94 182
Fragments arranged by size:
291 182 114 94 91 68
BseYI C'CCAG_C
Cuts at: 0 6 367 544 840
Size: 6 361 177 296
Fragments arranged by size:
361 296 177 6
BsiHKAI G_wGCw'C
Cuts at: 0 397 832 840
Size: 397 435 8
Fragments arranged by size:
435 397 8
BsmI GAATG_Cn'
Cuts at: 0 288 840
Size: 288 552
Bsp1286I G_dGCh'C
Cuts at: 0 45 292 339 381 397 832 840
Size: 45 247 47 42 16 435 8
Fragments arranged by size:
435 247 47 45 42 16 8
BspHI T'CATG_A
Cuts at: 0 659 840
Size: 659 181
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 10 840
Size: 10 830
BsrDI GCAATG_nn'
Cuts at: 0 462 840
Size: 462 378
BstXI CCAn_nnnn'nTGG
Cuts at: 0 448 840
Size: 448 392
BtgI C'CryG_G
Cuts at: 0 12 840
Size: 12 828
BtsI GCAGTG_nn'
Cuts at: 0 199 840
Size: 199 641
DrdI GACnn_nn'nnGTC
Cuts at: 0 472 840
Size: 472 368
EarI CTCTTCn'nnn_
Cuts at: 0 791 840
Size: 791 49
EcoICRI GAG'CTC
Cuts at: 0 830 840
Size: 830 10
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 298 840
Size: 298 542
EcoNI CCTnn'n_nnAGG
Cuts at: 0 661 840
Size: 661 179
EcoO109I rG'GnC_Cy
Cuts at: 0 41 272 551 840
Size: 41 231 279 289
Fragments arranged by size:
289 279 231 41
HaeII r_GCGC'y
Cuts at: 0 173 840
Size: 173 667
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 201 233 250 282 543 575 723 755
Size: 201 32 17 32 261 32 148 32
Cuts at: 755 840
Size: 85
Fragments arranged by size:
261 201 148 85 32 32 32 32
17
MslI CAynn'nnrTG
Cuts at: 0 50 719 840
Size: 50 669 121
Fragments arranged by size:
669 121 50
MspA1I CmG'CkG
Cuts at: 0 471 840
Size: 471 369
NspI r_CATG'y
Cuts at: 0 615 840
Size: 615 225
PasI CC'CwG_GG
Cuts at: 0 159 840
Size: 159 681
PpuMI rG'GwC_Cy
Cuts at: 0 272 551 840
Size: 272 279 289
Fragments arranged by size:
289 279 272
PspOMI G'GGCC_C
Cuts at: 0 41 840
Size: 41 799
PvuII CAG'CTG
Cuts at: 0 471 840
Size: 471 369
SacI G_AGCT'C
Cuts at: 0 832 840
Size: 832 8
SfcI C'TryA_G
Cuts at: 0 96 840
Size: 96 744
SmlI C'TyrA_G
Cuts at: 0 173 303 840
Size: 173 130 537
Fragments arranged by size:
537 173 130
StyI C'CwwG_G
Cuts at: 0 104 186 840
Size: 104 82 654
Fragments arranged by size:
654 104 82
XhoI C'TCGA_G
Cuts at: 0 303 840
Size: 303 537
Enzymes that do cut:
AcuI AfeI AhdI AlwNI ApaI AvaI AvrII BanI
BanII BbsI BbvCI BlpI Bme1580I Bpu10I BpuEI BsaI
BseRI BseYI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI
BstXI BtgI BtsI DrdI EarI EcoICRI Eco57MI EcoNI
EcoO109I HaeII Hin4I MslI MspA1I NspI PasI PpuMI
PspOMI PvuII SacI SfcI SmlI StyI XhoI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AflIII AgeI
AleI AlfI AloI ApaLI ApoI AscI AseI AsiSI
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BmtI BplI BpmI BsaAI BsaBI
BsaHI BsaWI BsaXI BsgI BsiEI BsiWI BsmBI BspEI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EaeI EagI EciI EcoRI EcoRV FalI FseI FspI
FspAI HincII HindIII HpaI KasI KpnI MfeI MluI
MmeI MscI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PciI PflMI PfoI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TatI TstI Tth111I
XbaI XcmI XmaI XmnI ZraI