Restriction map for
pF1KE0489
(Linear) (Six-base) MAPSORT of: KIEE0489.seq Check: 6434 from: 1 to: 507
KIEE0489.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
Acc65I G'GTAC_C
Cuts at: 0 377 507
Size: 377 130
AleI CACnn'nnGTG
Cuts at: 0 367 507
Size: 367 140
ApaI G_GGCC'C
Cuts at: 0 151 507
Size: 151 356
BanI G'GyrC_C
Cuts at: 0 15 39 125 301 377 507
Size: 15 24 86 176 76 130
Fragments arranged by size:
176 130 86 76 24 15
BanII G_rGCy'C
Cuts at: 0 67 151 391 507
Size: 67 84 240 116
Fragments arranged by size:
240 116 84 67
BbeI G_GCGC'C
Cuts at: 0 43 507
Size: 43 464
BglI GCCn_nnn'nGGC
Cuts at: 0 123 430 507
Size: 123 307 77
Fragments arranged by size:
307 123 77
Bme1580I G_kGCm'C
Cuts at: 0 20 151 163 306 507
Size: 20 131 12 143 201
Fragments arranged by size:
201 143 131 20 12
BmrI ACTGGGnnnn_n'
Cuts at: 0 147 266 285 507
Size: 147 119 19 222
Fragments arranged by size:
222 147 119 19
BsaI GGTCTCn'nnnn_
Cuts at: 0 94 507
Size: 94 413
BsaHI Gr'CG_yC
Cuts at: 0 40 507
Size: 40 467
BseYI C'CCAG_C
Cuts at: 0 43 144 210 317 507
Size: 43 101 66 107 190
Fragments arranged by size:
190 107 101 66 43
Bsp1286I G_dGCh'C
Cuts at: 0 20 67 151 163 306 391 507
Size: 20 47 84 12 143 85 116
Fragments arranged by size:
143 116 85 84 47 20 12
BsrFI r'CCGG_y
Cuts at: 0 103 507
Size: 103 404
BssSI C'ACGA_G
Cuts at: 0 120 507
Size: 120 387
BstXI CCAn_nnnn'nTGG
Cuts at: 0 369 507
Size: 369 138
BstYI r'GATC_y
Cuts at: 0 330 507
Size: 330 177
BtgI C'CryG_G
Cuts at: 0 23 78 176 368 474 507
Size: 23 55 98 192 106 33
Fragments arranged by size:
192 106 98 55 33 23
DraIII CAC_nnn'GTG
Cuts at: 0 141 507
Size: 141 366
EaeI y'GGCC_r
Cuts at: 0 395 507
Size: 395 112
EcoO109I rG'GnC_Cy
Cuts at: 0 148 507
Size: 148 359
HaeII r_GCGC'y
Cuts at: 0 43 507
Size: 43 464
KasI G'GCGC_C
Cuts at: 0 39 507
Size: 39 468
KpnI G_GTAC'C
Cuts at: 0 381 507
Size: 381 126
MslI CAynn'nnrTG
Cuts at: 0 367 507
Size: 367 140
MspA1I CmG'CkG
Cuts at: 0 95 264 393 507
Size: 95 169 129 114
Fragments arranged by size:
169 129 114 95
NaeI GCC'GGC
Cuts at: 0 105 507
Size: 105 402
NarI GG'CG_CC
Cuts at: 0 40 507
Size: 40 467
NcoI C'CATG_G
Cuts at: 0 23 176 474 507
Size: 23 153 298 33
Fragments arranged by size:
298 153 33 23
NgoMIV G'CCGG_C
Cuts at: 0 103 507
Size: 103 404
PasI CC'CwG_GG
Cuts at: 0 11 243 507
Size: 11 232 264
Fragments arranged by size:
264 232 11
PspOMI G'GGCC_C
Cuts at: 0 147 507
Size: 147 360
PstI C_TGCA'G
Cuts at: 0 113 507
Size: 113 394
PvuII CAG'CTG
Cuts at: 0 264 507
Size: 264 243
SexAI A'CCwGG_T
Cuts at: 0 194 380 507
Size: 194 186 127
Fragments arranged by size:
194 186 127
SfcI C'TryA_G
Cuts at: 0 109 507
Size: 109 398
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 430 507
Size: 430 77
SfoI GGC'GCC
Cuts at: 0 41 507
Size: 41 466
StyI C'CwwG_G
Cuts at: 0 23 176 323 474 507
Size: 23 153 147 151 33
Fragments arranged by size:
153 151 147 33 23
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3 129 507
Size: 3 126 378
Fragments arranged by size:
378 126 3
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 481 507
Size: 481 26
Enzymes that do cut:
Acc65I AleI ApaI BanI BanII BbeI BglI Bme1580I
BmrI BsaI BsaHI BseYI Bsp1286I BsrFI BssSI BstXI
BstYI BtgI DraIII EaeI EcoO109I HaeII KasI KpnI
MslI MspA1I NaeI NarI NcoI NgoMIV PasI PspOMI
PstI PvuII SexAI SfcI SfiI SfoI StyI TaqII
XcmI
Enzymes that do not cut:
AarI AatII AccI AclI AcuI AfeI AflII AflIII
AgeI AhdI AlfI AloI AlwNI ApaLI ApoI AscI
AseI AsiSI AvaI AvrII BaeI BamHI BbsI BbvCI
BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmtI
BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaWI BsaXI
BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BspMI BsrBI BsrDI BsrGI BssHII BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DrdI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI
EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII
HpaI MfeI MluI MmeI MscI NdeI NheI NotI
NruI NsiI NspI PacI PciI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI SwaI TatI TstI Tth111I XbaI XhoI
XmaI XmnI ZraI