(Linear) (Six-base) MAPSORT of: KIEE0483.seq  Check: 4349  from: 1  to: 1029



KIEE0483.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:15  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     639    1029 
   Size:        639     390
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     796    1029 
   Size:        796     233
 
AlwNI CAG_nnn'CTG

Cuts at:      0     358     499    1029 
   Size:        358     141     530

  Fragments arranged by size: 

                 530     358     141
 
ApoI r'AATT_y

Cuts at:      0     133     217    1029 
   Size:        133      84     812

  Fragments arranged by size: 

                 812     133      84
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      83     434     601    1029 
   Size:         83     351     167     428

  Fragments arranged by size: 

                 428     351     167      83
 
BglII A'GATC_T

Cuts at:      0      99     153    1029 
   Size:         99      54     876

  Fragments arranged by size: 

                 876      99      54
 
Bme1580I G_kGCm'C

Cuts at:      0     186    1029 
   Size:        186     843
 
BmgBI CAC'GTC

Cuts at:      0      81    1029 
   Size:         81     948
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     932    1029 
   Size:        932      97
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     853    1029 
   Size:        284     569     176

  Fragments arranged by size: 

                 569     284     176
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     955     985    1029 
   Size:        955      30      44

  Fragments arranged by size: 

                 955      44      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      56     323     621    1029 
   Size:         56     267     298     408

  Fragments arranged by size: 

                 408     298     267      56
 
BseYI C'CCAG_C

Cuts at:      0     297     358     705     857    1029 
   Size:        297      61     347     152     172

  Fragments arranged by size: 

                 347     297     172     152      61
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     667    1029 
   Size:        667     362
 
Bsp1286I G_dGCh'C

Cuts at:      0     186    1029 
   Size:        186     843
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     639     937    1029 
   Size:        639     298      92

  Fragments arranged by size: 

                 639     298      92
 
BsrGI T'GTAC_A

Cuts at:      0    1017    1029 
   Size:       1017      12
 
BstEII G'GTnAC_C

Cuts at:      0     363    1029 
   Size:        363     666
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     825     906    1029 
   Size:        825      81     123

  Fragments arranged by size: 

                 825     123      81
 
BstYI r'GATC_y

Cuts at:      0      99     153    1029 
   Size:         99      54     876

  Fragments arranged by size: 

                 876      99      54
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     697    1029 
   Size:        697     332
 
EaeI y'GGCC_r

Cuts at:      0     239     590     621     746    1029 
   Size:        239     351      31     125     283

  Fragments arranged by size: 

                 351     283     239     125      31
 
EarI CTCTTCn'nnn_

Cuts at:      0     467     800    1029 
   Size:        467     333     229

  Fragments arranged by size: 

                 467     333     229
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0      28     439     711    1029 
   Size:         28     411     272     318

  Fragments arranged by size: 

                 411     318     272      28
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     284     796     853    1029 
   Size:        284     512      57     176

  Fragments arranged by size: 

                 512     284     176      57
 
EcoO109I rG'GnC_Cy

Cuts at:      0     926    1029 
   Size:        926     103
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     368    1029 
   Size:        368     661
 
MscI TGG'CCA

Cuts at:      0     241     592     623     748    1029 
   Size:        241     351      31     125     281

  Fragments arranged by size: 

                 351     281     241     125      31
 
MspA1I CmG'CkG

Cuts at:      0     334     358     496    1029 
   Size:        334      24     138     533

  Fragments arranged by size: 

                 533     334     138      24
 
NspI r_CATG'y

Cuts at:      0     970    1029 
   Size:        970      59
 
PfoI T'CCnGG_A

Cuts at:      0     634    1029 
   Size:        634     395
 
PpuMI rG'GwC_Cy

Cuts at:      0     926    1029 
   Size:        926     103
 
PvuII CAG'CTG

Cuts at:      0     358     496    1029 
   Size:        358     138     533

  Fragments arranged by size: 

                 533     358     138
 
SspI AAT'ATT

Cuts at:      0     122    1029 
   Size:        122     907
 
StuI AGG'CCT

Cuts at:      0     661     823    1029 
   Size:        661     162     206

  Fragments arranged by size: 

                 661     206     162
 
StyI C'CwwG_G

Cuts at:      0     224     732     818     824    1029 
   Size:        224     508      86       6     205

  Fragments arranged by size: 

                 508     224     205      86       6
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     871    1029 
   Size:        871     158
 
TatI w'GTAC_w

Cuts at:      0    1017    1029 
   Size:       1017      12
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     955     987    1029 
   Size:        955      32      42

  Fragments arranged by size: 

                 955      42      32
 
Tth111I GACn'n_nGTC

Cuts at:      0     487    1029 
   Size:        487     542
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     231     585    1029 
   Size:        231     354     444

  Fragments arranged by size: 

                 444     354     231
 

 Enzymes that do cut:

     AarI     AcuI    AlwNI     ApoI     BbsI    BglII Bme1580I    BmgBI
     BmrI     BpmI    BsaXI    BseRI    BseYI     BsgI Bsp1286I    BspMI
    BsrGI   BstEII    BstXI    BstYI    BtgZI     EaeI     EarI     EciI
  Eco57MI EcoO109I     MmeI     MscI   MspA1I     NspI     PfoI    PpuMI
    PvuII     SspI     StuI     StyI    TaqII     TatI     TstI  Tth111I
     XcmI

 Enzymes that do not cut: 

    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI     BlpI     BmtI
     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI
    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI    BspEI    BspHI    BsrBI
    BsrDI    BsrFI   BssHII    BssSI   BstAPI    BstBI  BstZ17I   Bsu36I
     BtgI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EagI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     PacI     PasI     PciI    PflMI     PmeI
     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI
     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI     XbaI     XhoI
     XmaI     XmnI     ZraI