Restriction map for
pF1KE0479
(Linear) (Six-base) MAPSORT of: KIEE0479.seq Check: 167 from: 1 to: 945
KIEE0479.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 945
Size: 185 760
AfeI AGC'GCT
Cuts at: 0 307 945
Size: 307 638
AflIII A'CryG_T
Cuts at: 0 647 945
Size: 647 298
AlwNI CAG_nnn'CTG
Cuts at: 0 427 945
Size: 427 518
ApaLI G'TGCA_C
Cuts at: 0 36 945
Size: 36 909
ApoI r'AATT_y
Cuts at: 0 292 945
Size: 292 653
BamHI G'GATC_C
Cuts at: 0 770 945
Size: 770 175
BanI G'GyrC_C
Cuts at: 0 812 945
Size: 812 133
BanII G_rGCy'C
Cuts at: 0 278 945
Size: 278 667
BbvCI CC'TCA_GC
Cuts at: 0 95 945
Size: 95 850
BclI T'GATC_A
Cuts at: 0 183 789 945
Size: 183 606 156
Fragments arranged by size:
606 183 156
Bme1580I G_kGCm'C
Cuts at: 0 40 945
Size: 40 905
Bpu10I CC'TnA_GC
Cuts at: 0 95 945
Size: 95 850
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 72 945
Size: 72 873
BsaI GGTCTCn'nnnn_
Cuts at: 0 494 751 945
Size: 494 257 194
Fragments arranged by size:
494 257 194
BsaBI GATnn'nnATC
Cuts at: 0 422 945
Size: 422 523
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 394 535 562 877 945
Size: 394 141 27 315 68
Fragments arranged by size:
394 315 141 68 27
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 523 945
Size: 523 422
BsiHKAI G_wGCw'C
Cuts at: 0 40 278 945
Size: 40 238 667
Fragments arranged by size:
667 238 40
BsmI GAATG_Cn'
Cuts at: 0 612 945
Size: 612 333
Bsp1286I G_dGCh'C
Cuts at: 0 40 278 945
Size: 40 238 667
Fragments arranged by size:
667 238 40
BspHI T'CATG_A
Cuts at: 0 87 945
Size: 87 858
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 14 945
Size: 14 931
BsrBI CCG'CTC
Cuts at: 0 471 945
Size: 471 474
BsrDI GCAATG_nn'
Cuts at: 0 506 945
Size: 506 439
BsrGI T'GTAC_A
Cuts at: 0 81 650 945
Size: 81 569 295
Fragments arranged by size:
569 295 81
BstXI CCAn_nnnn'nTGG
Cuts at: 0 816 945
Size: 816 129
BstYI r'GATC_y
Cuts at: 0 423 770 945
Size: 423 347 175
Fragments arranged by size:
423 347 175
Bsu36I CC'TnA_GG
Cuts at: 0 545 945
Size: 545 400
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 894 929 945
Size: 894 35 16
Fragments arranged by size:
894 35 16
DraI TTT'AAA
Cuts at: 0 480 945
Size: 480 465
DrdI GACnn_nn'nnGTC
Cuts at: 0 259 945
Size: 259 686
EaeI y'GGCC_r
Cuts at: 0 141 945
Size: 141 804
EarI CTCTTCn'nnn_
Cuts at: 0 194 626 749 945
Size: 194 432 123 196
Fragments arranged by size:
432 196 194 123
EcoICRI GAG'CTC
Cuts at: 0 276 945
Size: 276 669
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 185 945
Size: 185 760
EcoNI CCTnn'n_nnAGG
Cuts at: 0 355 945
Size: 355 590
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 900 945
Size: 900 45
HaeII r_GCGC'y
Cuts at: 0 309 945
Size: 309 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 168 200 945
Size: 168 32 745
Fragments arranged by size:
745 168 32
HincII GTy'rAC
Cuts at: 0 873 945
Size: 873 72
HindIII A'AGCT_T
Cuts at: 0 850 945
Size: 850 95
MscI TGG'CCA
Cuts at: 0 143 945
Size: 143 802
MslI CAynn'nnrTG
Cuts at: 0 119 945
Size: 119 826
MspA1I CmG'CkG
Cuts at: 0 189 945
Size: 189 756
NspI r_CATG'y
Cuts at: 0 651 945
Size: 651 294
PciI A'CATG_T
Cuts at: 0 647 945
Size: 647 298
PflMI CCAn_nnn'nTGG
Cuts at: 0 767 945
Size: 767 178
PvuII CAG'CTG
Cuts at: 0 189 945
Size: 189 756
SacI G_AGCT'C
Cuts at: 0 278 945
Size: 278 667
SexAI A'CCwGG_T
Cuts at: 0 815 945
Size: 815 130
SmlI C'TyrA_G
Cuts at: 0 51 945
Size: 51 894
StuI AGG'CCT
Cuts at: 0 922 945
Size: 922 23
StyI C'CwwG_G
Cuts at: 0 515 857 945
Size: 515 342 88
Fragments arranged by size:
515 342 88
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 148 945
Size: 148 797
TatI w'GTAC_w
Cuts at: 0 81 576 650 945
Size: 81 495 74 295
Fragments arranged by size:
495 295 81 74
XbaI T'CTAG_A
Cuts at: 0 627 945
Size: 627 318
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 841 945
Size: 841 104
Enzymes that do cut:
AcuI AfeI AflIII AlwNI ApaLI ApoI BamHI BanI
BanII BbvCI BclI Bme1580I Bpu10I BpuEI BsaI BsaBI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrDI BsrGI BstXI BstYI Bsu36I CspCI DraI DrdI
EaeI EarI EcoICRI Eco57MI EcoNI FalI HaeII Hin4I
HincII HindIII MscI MslI MspA1I NspI PciI PflMI
PvuII SacI SexAI SmlI StuI StyI TaqII TatI
XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvaI
AvrII BaeI BbeI BbsI BcgI BciVI BfrBI BglI
BglII BlpI BmgBI BmrI BmtI BplI BpmI BsaAI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmBI BspEI
BsrFI BssHII BssSI BstAPI BstBI BstEII BstZ17I BtgI
BtgZI BtsI ClaI DraIII EagI EciI EcoO109I EcoRI
EcoRV FseI FspI FspAI HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PasI PfoI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacII SalI SanDI SapI SbfI ScaI
SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XhoI XmaI
XmnI ZraI