Restriction map for
pF1KE0478
(Linear) (Six-base) MAPSORT of: KIEE0478.seq Check: 9840 from: 1 to: 1125
KIEE0478.orf
With 182 enzymes: SgfI *
April 24, 2009 13:15 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 730 1125
Size: 730 395
AfeI AGC'GCT
Cuts at: 0 683 804 1125
Size: 683 121 321
Fragments arranged by size:
683 321 121
AflIII A'CryG_T
Cuts at: 0 452 1125
Size: 452 673
AleI CACnn'nnGTG
Cuts at: 0 715 1125
Size: 715 410
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 68 1125
Size: 68 1057
AlwNI CAG_nnn'CTG
Cuts at: 0 315 1125
Size: 315 810
ApaI G_GGCC'C
Cuts at: 0 1113 1125
Size: 1113 12
ApaLI G'TGCA_C
Cuts at: 0 43 1055 1125
Size: 43 1012 70
Fragments arranged by size:
1012 70 43
AvaI C'yCGr_G
Cuts at: 0 367 955 1112 1125
Size: 367 588 157 13
Fragments arranged by size:
588 367 157 13
BanI G'GyrC_C
Cuts at: 0 322 388 870 933 1125
Size: 322 66 482 63 192
Fragments arranged by size:
482 322 192 66 63
BanII G_rGCy'C
Cuts at: 0 1027 1113 1125
Size: 1027 86 12
Fragments arranged by size:
1027 86 12
BbeI G_GCGC'C
Cuts at: 0 326 937 1125
Size: 326 611 188
Fragments arranged by size:
611 326 188
BbvCI CC'TCA_GC
Cuts at: 0 122 766 1125
Size: 122 644 359
Fragments arranged by size:
644 359 122
BfrBI ATG'CAT
Cuts at: 0 815 1125
Size: 815 310
BglI GCCn_nnn'nGGC
Cuts at: 0 580 730 1125
Size: 580 150 395
Fragments arranged by size:
580 395 150
BlpI GC'TnA_GC
Cuts at: 0 308 1125
Size: 308 817
Bme1580I G_kGCm'C
Cuts at: 0 47 1059 1113 1125
Size: 47 1012 54 12
Fragments arranged by size:
1012 54 47 12
BmgBI CAC'GTC
Cuts at: 0 48 1125
Size: 48 1077
BmrI ACTGGGnnnn_n'
Cuts at: 0 628 1125
Size: 628 497
BmtI G_CTAG'C
Cuts at: 0 340 1125
Size: 340 785
Bpu10I CC'TnA_GC
Cuts at: 0 122 766 1125
Size: 122 644 359
Fragments arranged by size:
644 359 122
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 337 487 499 1125
Size: 337 150 12 626
Fragments arranged by size:
626 337 150 12
BsaI GGTCTCn'nnnn_
Cuts at: 0 193 1125
Size: 193 932
BsaHI Gr'CG_yC
Cuts at: 0 227 323 706 934 1125
Size: 227 96 383 228 191
Fragments arranged by size:
383 228 227 191 96
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 381 411 1125
Size: 25 356 30 714
Fragments arranged by size:
714 356 30 25
BseYI C'CCAG_C
Cuts at: 0 856 1047 1125
Size: 856 191 78
Fragments arranged by size:
856 191 78
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 766 1125
Size: 766 359
BsiHKAI G_wGCw'C
Cuts at: 0 47 905 1059 1125
Size: 47 858 154 66
Fragments arranged by size:
858 154 66 47
BsmBI CGTCTCn'nnnn_
Cuts at: 0 54 697 1125
Size: 54 643 428
Fragments arranged by size:
643 428 54
Bsp1286I G_dGCh'C
Cuts at: 0 47 905 1027 1059 1113 1125
Size: 47 858 122 32 54 12
Fragments arranged by size:
858 122 54 47 32 12
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 730 1125
Size: 730 395
BsrDI GCAATG_nn'
Cuts at: 0 527 1125
Size: 527 598
BsrFI r'CCGG_y
Cuts at: 0 181 385 1125
Size: 181 204 740
Fragments arranged by size:
740 204 181
BssHII G'CGCG_C
Cuts at: 0 6 783 1125
Size: 6 777 342
Fragments arranged by size:
777 342 6
BssSI C'ACGA_G
Cuts at: 0 1018 1125
Size: 1018 107
BstYI r'GATC_y
Cuts at: 0 173 1125
Size: 173 952
BtgI C'CryG_G
Cuts at: 0 235 1125
Size: 235 890
BtsI GCAGTG_nn'
Cuts at: 0 502 1125
Size: 502 623
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 405 440 1125
Size: 405 35 685
Fragments arranged by size:
685 405 35
EaeI y'GGCC_r
Cuts at: 0 665 1125
Size: 665 460
EcoO109I rG'GnC_Cy
Cuts at: 0 372 1109 1125
Size: 372 737 16
Fragments arranged by size:
737 372 16
FspI TGC'GCA
Cuts at: 0 688 1125
Size: 688 437
HaeII r_GCGC'y
Cuts at: 0 326 685 806 830 937 1125
Size: 326 359 121 24 107 188
Fragments arranged by size:
359 326 188 121 107 24
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 627 659 1125
Size: 27 600 32 466
Fragments arranged by size:
600 466 32 27
KasI G'GCGC_C
Cuts at: 0 322 933 1125
Size: 322 611 192
Fragments arranged by size:
611 322 192
MslI CAynn'nnrTG
Cuts at: 0 697 715 1125
Size: 697 18 410
Fragments arranged by size:
697 410 18
MspA1I CmG'CkG
Cuts at: 0 39 98 237 402 914 1125
Size: 39 59 139 165 512 211
Fragments arranged by size:
512 211 165 139 59 39
NaeI GCC'GGC
Cuts at: 0 183 1125
Size: 183 942
NarI GG'CG_CC
Cuts at: 0 323 934 1125
Size: 323 611 191
Fragments arranged by size:
611 323 191
NgoMIV G'CCGG_C
Cuts at: 0 181 1125
Size: 181 944
NheI G'CTAG_C
Cuts at: 0 336 1125
Size: 336 789
NsiI A_TGCA'T
Cuts at: 0 817 1125
Size: 817 308
NspI r_CATG'y
Cuts at: 0 819 1125
Size: 819 306
PfoI T'CCnGG_A
Cuts at: 0 612 1125
Size: 612 513
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 350 382 1125
Size: 350 32 743
Fragments arranged by size:
743 350 32
PpuMI rG'GwC_Cy
Cuts at: 0 372 1125
Size: 372 753
PspOMI G'GGCC_C
Cuts at: 0 1109 1125
Size: 1109 16
PspXI vC'TCGA_Gb
Cuts at: 0 367 1125
Size: 367 758
PstI C_TGCA'G
Cuts at: 0 162 494 1125
Size: 162 332 631
Fragments arranged by size:
631 332 162
PvuII CAG'CTG
Cuts at: 0 914 1125
Size: 914 211
SacII CC_GC'GG
Cuts at: 0 238 1125
Size: 238 887
SfcI C'TryA_G
Cuts at: 0 158 490 1125
Size: 158 332 635
Fragments arranged by size:
635 332 158
SfoI GGC'GCC
Cuts at: 0 324 935 1125
Size: 324 611 190
Fragments arranged by size:
611 324 190
SmaI CCC'GGG
Cuts at: 0 1114 1125
Size: 1114 11
SmlI C'TyrA_G
Cuts at: 0 352 367 502 514 1125
Size: 352 15 135 12 611
Fragments arranged by size:
611 352 135 15 12
StuI AGG'CCT
Cuts at: 0 512 1125
Size: 512 613
StyI C'CwwG_G
Cuts at: 0 1104 1125
Size: 1104 21
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 381 413 1079 1111 1125
Size: 381 32 666 32 14
Fragments arranged by size:
666 381 32 32 14
Tth111I GACn'n_nGTC
Cuts at: 0 253 1068 1125
Size: 253 815 57
Fragments arranged by size:
815 253 57
XhoI C'TCGA_G
Cuts at: 0 367 1125
Size: 367 758
XmaI C'CCGG_G
Cuts at: 0 1112 1125
Size: 1112 13
XmnI GAAnn'nnTTC
Cuts at: 0 566 988 1125
Size: 566 422 137
Fragments arranged by size:
566 422 137
Enzymes that do cut:
AarI AfeI AflIII AleI AlfI AlwNI ApaI ApaLI
AvaI BanI BanII BbeI BbvCI BfrBI BglI BlpI
Bme1580I BmgBI BmrI BmtI Bpu10I BpuEI BsaI BsaHI
BsaXI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrDI
BsrFI BssHII BssSI BstYI BtgI BtsI CspCI EaeI
EcoO109I FspI HaeII Hin4I KasI MslI MspA1I NaeI
NarI NgoMIV NheI NsiI NspI PfoI PpiI PpuMI
PspOMI PspXI PstI PvuII SacII SfcI SfoI SmaI
SmlI StuI StyI TstI Tth111I XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AloI ApoI AscI AseI AsiSI AvrII BaeI BamHI
BbsI BcgI BciVI BclI BglII BplI BpmI BsaAI
BsaBI BsaWI BseRI BsiEI BsiWI BsmI BspEI BspHI
BsrBI BsrGI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI DraI DraIII DrdI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspAI
HincII HindIII HpaI KpnI MfeI MluI MmeI MscI
NcoI NdeI NotI NruI PacI PasI PciI PflMI
PmeI PmlI PshAI PsiI PsrI PvuI RsrII SacI
SalI SanDI SapI SbfI ScaI SexAI SfiI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI TaqII
TatI XbaI XcmI ZraI