(Linear) (Six-base) MAPSORT of: KIEE0474.seq  Check: 4344  from: 1  to: 501



KIEE0474.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     453     501 
   Size:        453      48
 
AflII C'TTAA_G

Cuts at:      0     100     501 
   Size:        100     401
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     320     501 
   Size:        320     181
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     167     199     501 
   Size:        167      32     302

  Fragments arranged by size: 

                 302     167      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0      16     501 
   Size:         16     485
 
ApoI r'AATT_y

Cuts at:      0     262     501 
   Size:        262     239
 
BanI G'GyrC_C

Cuts at:      0     321     501 
   Size:        321     180
 
BanII G_rGCy'C

Cuts at:      0     213     501 
   Size:        213     288
 
BglII A'GATC_T

Cuts at:      0      23     501 
   Size:         23     478
 
Bme1580I G_kGCm'C

Cuts at:      0     324     501 
   Size:        324     177
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     501 
   Size:        143     358
 
BsiHKAI G_wGCw'C

Cuts at:      0     213     501 
   Size:        213     288
 
Bsp1286I G_dGCh'C

Cuts at:      0     213     324     501 
   Size:        213     111     177

  Fragments arranged by size: 

                 213     177     111
 
BsrDI GCAATG_nn'

Cuts at:      0     486     501 
   Size:        486      15
 
BstYI r'GATC_y

Cuts at:      0      23     501 
   Size:         23     478
 
BtsI GCAGTG_nn'

Cuts at:      0     299     501 
   Size:        299     202
 
EaeI y'GGCC_r

Cuts at:      0     193     501 
   Size:        193     308
 
EarI CTCTTCn'nnn_

Cuts at:      0     266     501 
   Size:        266     235
 
EcoICRI GAG'CTC

Cuts at:      0     211     501 
   Size:        211     290
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     453     501 
   Size:        143     310      48

  Fragments arranged by size: 

                 310     143      48
 
MscI TGG'CCA

Cuts at:      0     195     501 
   Size:        195     306
 
MslI CAynn'nnrTG

Cuts at:      0     491     501 
   Size:        491      10
 
MspA1I CmG'CkG

Cuts at:      0     426     501 
   Size:        426      75
 
NdeI CA'TA_TG

Cuts at:      0     147     501 
   Size:        147     354
 
NspI r_CATG'y

Cuts at:      0      42     501 
   Size:         42     459
 
PsiI TTA'TAA

Cuts at:      0     388     501 
   Size:        388     113
 
PvuII CAG'CTG

Cuts at:      0     426     501 
   Size:        426      75
 
SacI G_AGCT'C

Cuts at:      0     213     501 
   Size:        213     288
 
SapI GCTCTTCn'nnn_

Cuts at:      0     266     501 
   Size:        266     235
 
SmlI C'TyrA_G

Cuts at:      0     100     501 
   Size:        100     401
 
XbaI T'CTAG_A

Cuts at:      0     186     501 
   Size:        186     315
 

 Enzymes that do cut:

     AcuI    AflII     AlfI     AloI    AlwNI     ApoI     BanI    BanII
    BglII Bme1580I     BpmI  BsiHKAI Bsp1286I    BsrDI    BstYI     BtsI
     EaeI     EarI  EcoICRI  Eco57MI     MscI     MslI   MspA1I     NdeI
     NspI     PsiI    PvuII     SacI     SapI     SmlI     XbaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     ApaI    ApaLI     AscI     AseI    AsiSI     AvaI
    AvrII     BaeI    BamHI     BbeI     BbsI    BbvCI     BcgI    BciVI
     BclI    BfrBI     BglI     BlpI    BmgBI     BmrI     BmtI     BplI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI
    BspHI    BspMI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI
    CspCI     DraI   DraIII     DrdI     EagI     EciI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     NaeI     NarI     NcoI   NgoMIV     NheI     NotI     NruI     NsiI
     PacI     PasI     PciI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PstI     PvuI    RsrII
    SacII     SalI    SanDI     SbfI     ScaI    SexAI     SfcI     SfiI
     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI  Tth111I
     XcmI     XhoI     XmaI     XmnI     ZraI