(Linear) (Six-base) MAPSORT of: KIEE0472.seq  Check: 1432  from: 1  to: 558



KIEE0472.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     394     558 
   Size:        394     164
 
BclI T'GATC_A

Cuts at:      0     269     558 
   Size:        269     289
 
BmtI G_CTAG'C

Cuts at:      0     511     558 
   Size:        511      47
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0      93     192     558 
   Size:         93      99     366

  Fragments arranged by size: 

                 366      99      93
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     558 
   Size:         60     498
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     166     196     558 
   Size:        166      30     362

  Fragments arranged by size: 

                 362     166      30
 
BseYI C'CCAG_C

Cuts at:      0     313     483     487     558 
   Size:        313     170       4      71

  Fragments arranged by size: 

                 313     170      71       4
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     111     299     558 
   Size:        111     188     259

  Fragments arranged by size: 

                 259     188     111
 
BsiHKAI G_wGCw'C

Cuts at:      0     173     558 
   Size:        173     385
 
Bsp1286I G_dGCh'C

Cuts at:      0     173     558 
   Size:        173     385
 
BspHI T'CATG_A

Cuts at:      0     158     558 
   Size:        158     400
 
BtgI C'CryG_G

Cuts at:      0     207     558 
   Size:        207     351
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     240     558 
   Size:        240     318
 
BtsI GCAGTG_nn'

Cuts at:      0     395     558 
   Size:        395     163
 
EarI CTCTTCn'nnn_

Cuts at:      0      62     558 
   Size:         62     496
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      60     558 
   Size:         60     498
 
HindIII A'AGCT_T

Cuts at:      0     261     558 
   Size:        261     297
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     513     558 
   Size:        513      45
 
MslI CAynn'nnrTG

Cuts at:      0     421     558 
   Size:        421     137
 
MspA1I CmG'CkG

Cuts at:      0     487     558 
   Size:        487      71
 
NheI G'CTAG_C

Cuts at:      0     507     558 
   Size:        507      51
 
NspI r_CATG'y

Cuts at:      0     136     558 
   Size:        136     422
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      86     390     558 
   Size:         86     304     168

  Fragments arranged by size: 

                 304     168      86
 
PstI C_TGCA'G

Cuts at:      0     198     339     558 
   Size:        198     141     219

  Fragments arranged by size: 

                 219     198     141
 
PvuII CAG'CTG

Cuts at:      0     487     558 
   Size:        487      71
 
SfcI C'TryA_G

Cuts at:      0     194     248     335     558 
   Size:        194      54      87     223

  Fragments arranged by size: 

                 223     194      87      54
 
SspI AAT'ATT

Cuts at:      0      84     558 
   Size:         84     474
 

 Enzymes that do cut:

    AlwNI     BclI     BmtI     BplI     BpmI    BsaXI    BseYI     BsgI
  BsiHKAI Bsp1286I    BspHI     BtgI    BtgZI     BtsI     EarI  Eco57MI
  HindIII     MmeI     MslI   MspA1I     NheI     NspI    PflMI     PstI
    PvuII     SfcI     SspI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI    BfrBI
     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI    BsiEI    BsiWI
     BsmI    BsmBI    BspEI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I
   Bsu36I     ClaI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EciI  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI
     FspI    FspAI    HaeII    Hin4I   HincII     HpaI     KasI     KpnI
     MfeI     MluI     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV
     NotI     NruI     NsiI     PacI     PasI     PciI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI
     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     StuI     StyI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI