(Linear) (Six-base) MAPSORT of: KIEE0461.seq  Check: 6181  from: 1  to: 645



KIEE0461.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     566     645 
   Size:        449     117      79

  Fragments arranged by size: 

                 449     117      79
 
AflII C'TTAA_G

Cuts at:      0     303     645 
   Size:        303     342
 
ApoI r'AATT_y

Cuts at:      0     103     139     645 
   Size:        103      36     506

  Fragments arranged by size: 

                 506     103      36
 
BanII G_rGCy'C

Cuts at:      0     261     645 
   Size:        261     384
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     442     476     645 
   Size:        442      34     169

  Fragments arranged by size: 

                 442     169      34
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     189     645 
   Size:        189     456
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     517     645 
   Size:        517     128
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     464     494     645 
   Size:        464      30     151

  Fragments arranged by size: 

                 464     151      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     260     645 
   Size:        260     385
 
BsiHKAI G_wGCw'C

Cuts at:      0     261     645 
   Size:        261     384
 
BsmI GAATG_Cn'

Cuts at:      0     555     645 
   Size:        555      90
 
Bsp1286I G_dGCh'C

Cuts at:      0     261     645 
   Size:        261     384
 
BsrFI r'CCGG_y

Cuts at:      0     599     645 
   Size:        599      46
 
BsrGI T'GTAC_A

Cuts at:      0     567     645 
   Size:        567      78
 
BstYI r'GATC_y

Cuts at:      0     543     645 
   Size:        543     102
 
DraI TTT'AAA

Cuts at:      0      36     645 
   Size:         36     609
 
EaeI y'GGCC_r

Cuts at:      0     168     645 
   Size:        168     477
 
EcoICRI GAG'CTC

Cuts at:      0     259     645 
   Size:        259     386
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     449     566     645 
   Size:        449     117      79

  Fragments arranged by size: 

                 449     117      79
 
EcoRV GAT'ATC

Cuts at:      0     276     645 
   Size:        276     369
 
HincII GTy'rAC

Cuts at:      0     317     645 
   Size:        317     328
 
HindIII A'AGCT_T

Cuts at:      0     537     645 
   Size:        537     108
 
MfeI C'AATT_G

Cuts at:      0     128     645 
   Size:        128     517
 
MscI TGG'CCA

Cuts at:      0     170     645 
   Size:        170     475
 
MspA1I CmG'CkG

Cuts at:      0     311     645 
   Size:        311     334
 
NaeI GCC'GGC

Cuts at:      0     601     645 
   Size:        601      44
 
NdeI CA'TA_TG

Cuts at:      0     405     645 
   Size:        405     240
 
NgoMIV G'CCGG_C

Cuts at:      0     599     645 
   Size:        599      46
 
PstI C_TGCA'G

Cuts at:      0     188     645 
   Size:        188     457
 
PvuII CAG'CTG

Cuts at:      0     311     645 
   Size:        311     334
 
SacI G_AGCT'C

Cuts at:      0     261     645 
   Size:        261     384
 
SfcI C'TryA_G

Cuts at:      0     184     240     645 
   Size:        184      56     405

  Fragments arranged by size: 

                 405     184      56
 
SmlI C'TyrA_G

Cuts at:      0     303     496     645 
   Size:        303     193     149

  Fragments arranged by size: 

                 303     193     149
 
TatI w'GTAC_w

Cuts at:      0     289     567     645 
   Size:        289     278      78

  Fragments arranged by size: 

                 289     278      78
 

 Enzymes that do cut:

     AcuI    AflII     ApoI    BanII     BcgI    BciVI    BpuEI    BsaXI
    BseRI  BsiHKAI     BsmI Bsp1286I    BsrFI    BsrGI    BstYI     DraI
     EaeI  EcoICRI  Eco57MI    EcoRV   HincII  HindIII     MfeI     MscI
   MspA1I     NaeI     NdeI   NgoMIV     PstI    PvuII     SacI     SfcI
     SmlI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI
     BbsI    BbvCI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I     BsaI    BsaAI
    BsaBI    BsaHI    BsaWI    BseYI     BsgI    BsiEI    BsiWI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrDI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI   DraIII     DrdI     EagI     EarI     EciI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I
     HpaI     KasI     KpnI     MluI     MmeI     MslI     NarI     NcoI
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PvuI    RsrII    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI     StyI
     SwaI    TaqII     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI