Restriction map for
pF1KE0456
(Linear) (Six-base) MAPSORT of: KIEE0456.seq Check: 5796 from: 1 to: 822
KIEE0456.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AlwNI CAG_nnn'CTG
Cuts at: 0 75 822
Size: 75 747
ApaI G_GGCC'C
Cuts at: 0 582 822
Size: 582 240
AvaI C'yCGr_G
Cuts at: 0 342 822
Size: 342 480
BanI G'GyrC_C
Cuts at: 0 647 822
Size: 647 175
BanII G_rGCy'C
Cuts at: 0 342 582 822
Size: 342 240 240
Fragments arranged by size:
342 240 240
BbeI G_GCGC'C
Cuts at: 0 651 822
Size: 651 171
BbvCI CC'TCA_GC
Cuts at: 0 731 822
Size: 731 91
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 492 526 822
Size: 492 34 296
Fragments arranged by size:
492 296 34
BglI GCCn_nnn'nGGC
Cuts at: 0 151 656 811 822
Size: 151 505 155 11
Fragments arranged by size:
505 155 151 11
BlpI GC'TnA_GC
Cuts at: 0 592 822
Size: 592 230
Bme1580I G_kGCm'C
Cuts at: 0 248 582 680 822
Size: 248 334 98 142
Fragments arranged by size:
334 248 142 98
BmgBI CAC'GTC
Cuts at: 0 699 822
Size: 699 123
BmrI ACTGGGnnnn_n'
Cuts at: 0 645 822
Size: 645 177
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 822
Size: 52 770
Bpu10I CC'TnA_GC
Cuts at: 0 731 822
Size: 731 91
BsaAI yAC'GTr
Cuts at: 0 353 822
Size: 353 469
BsaBI GATnn'nnATC
Cuts at: 0 532 822
Size: 532 290
BsaHI Gr'CG_yC
Cuts at: 0 648 822
Size: 648 174
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 32 62 160 190 822
Size: 32 30 98 30 632
Fragments arranged by size:
632 98 32 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 109 822
Size: 40 69 713
Fragments arranged by size:
713 69 40
BseYI C'CCAG_C
Cuts at: 0 80 89 822
Size: 80 9 733
Fragments arranged by size:
733 80 9
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 361 547 822
Size: 361 186 275
Fragments arranged by size:
361 275 186
BsiEI CG_ry'CG
Cuts at: 0 796 822
Size: 796 26
BsiHKAI G_wGCw'C
Cuts at: 0 401 822
Size: 401 421
BsmBI CGTCTCn'nnnn_
Cuts at: 0 180 822
Size: 180 642
Bsp1286I G_dGCh'C
Cuts at: 0 248 342 401 582 680 822
Size: 248 94 59 181 98 142
Fragments arranged by size:
248 181 142 98 94 59
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 383 822
Size: 383 439
BsrBI CCG'CTC
Cuts at: 0 331 788 822
Size: 331 457 34
Fragments arranged by size:
457 331 34
BsrFI r'CCGG_y
Cuts at: 0 163 287 655 822
Size: 163 124 368 167
Fragments arranged by size:
368 167 163 124
BstAPI GCAn_nnn'nTGC
Cuts at: 0 558 822
Size: 558 264
BstXI CCAn_nnnn'nTGG
Cuts at: 0 420 822
Size: 420 402
BstYI r'GATC_y
Cuts at: 0 527 822
Size: 527 295
BtgI C'CryG_G
Cuts at: 0 413 759 822
Size: 413 346 63
Fragments arranged by size:
413 346 63
BtsI GCAGTG_nn'
Cuts at: 0 158 556 822
Size: 158 398 266
Fragments arranged by size:
398 266 158
DraIII CAC_nnn'GTG
Cuts at: 0 570 822
Size: 570 252
EaeI y'GGCC_r
Cuts at: 0 17 332 671 683 793 822
Size: 17 315 339 12 110 29
Fragments arranged by size:
339 315 110 29 17 12
EagI C'GGCC_G
Cuts at: 0 793 822
Size: 793 29
EarI CTCTTCn'nnn_
Cuts at: 0 49 781 822
Size: 49 732 41
Fragments arranged by size:
732 49 41
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 822
Size: 52 770
EcoNI CCTnn'n_nnAGG
Cuts at: 0 379 822
Size: 379 443
EcoO109I rG'GnC_Cy
Cuts at: 0 578 579 764 822
Size: 578 1 185 58
Fragments arranged by size:
578 185 58 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 483 822
Size: 483 339
HaeII r_GCGC'y
Cuts at: 0 651 713 822
Size: 651 62 109
Fragments arranged by size:
651 109 62
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 822
Size: 32 32 758
Fragments arranged by size:
758 32 32
HincII GTy'rAC
Cuts at: 0 702 822
Size: 702 120
KasI G'GCGC_C
Cuts at: 0 647 822
Size: 647 175
MscI TGG'CCA
Cuts at: 0 685 822
Size: 685 137
MslI CAynn'nnrTG
Cuts at: 0 487 822
Size: 487 335
MspA1I CmG'CkG
Cuts at: 0 72 822
Size: 72 750
NaeI GCC'GGC
Cuts at: 0 165 657 822
Size: 165 492 165
Fragments arranged by size:
492 165 165
NarI GG'CG_CC
Cuts at: 0 648 822
Size: 648 174
NcoI C'CATG_G
Cuts at: 0 413 822
Size: 413 409
NgoMIV G'CCGG_C
Cuts at: 0 163 655 822
Size: 163 492 167
Fragments arranged by size:
492 167 163
NspI r_CATG'y
Cuts at: 0 486 822
Size: 486 336
PmlI CAC'GTG
Cuts at: 0 353 822
Size: 353 469
PspOMI G'GGCC_C
Cuts at: 0 578 822
Size: 578 244
SexAI A'CCwGG_T
Cuts at: 0 299 822
Size: 299 523
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 151 811 822
Size: 151 660 11
Fragments arranged by size:
660 151 11
SfoI GGC'GCC
Cuts at: 0 649 822
Size: 649 173
SmaI CCC'GGG
Cuts at: 0 344 822
Size: 344 478
StyI C'CwwG_G
Cuts at: 0 413 519 822
Size: 413 106 303
Fragments arranged by size:
413 303 106
TatI w'GTAC_w
Cuts at: 0 539 632 822
Size: 539 93 190
Fragments arranged by size:
539 190 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 202 234 822
Size: 202 32 588
Fragments arranged by size:
588 202 32
Tth111I GACn'n_nGTC
Cuts at: 0 106 822
Size: 106 716
XmaI C'CCGG_G
Cuts at: 0 342 822
Size: 342 480
Enzymes that do cut:
AlwNI ApaI AvaI BanI BanII BbeI BbvCI BcgI
BglI BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaAI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmBI Bsp1286I BspMI BsrBI BsrFI BstAPI BstXI BstYI
BtgI BtsI DraIII EaeI EagI EarI Eco57MI EcoNI
EcoO109I FalI HaeII Hin4I HincII KasI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PmlI PspOMI
SexAI SfiI SfoI SmaI StyI TatI TstI Tth111I
XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbsI BciVI
BclI BfrBI BglII BmtI BplI BpuEI BsaI BsaWI
BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BssSI
BstBI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI DraI
DrdI EciI EcoICRI EcoRI EcoRV FseI FspI FspAI
HindIII HpaI KpnI MfeI MluI MmeI NdeI NheI
NotI NruI NsiI PacI PasI PciI PflMI PfoI
PmeI PpiI PpuMI PshAI PsiI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SfcI SgfI SgrAI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI TaqII XbaI XcmI
XhoI XmnI ZraI