(Linear) (Six-base) MAPSORT of: KIEE0444.seq  Check: 8542  from: 1  to: 831



KIEE0444.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AfeI AGC'GCT

Cuts at:      0     275     831 
   Size:        275     556
 
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     685     717     831 
   Size:        685      32     114

  Fragments arranged by size: 

                 685     114      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     736     831 
   Size:        736      95
 
AvaI C'yCGr_G

Cuts at:      0     269     831 
   Size:        269     562
 
BanI G'GyrC_C

Cuts at:      0     577     831 
   Size:        577     254
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      20     831 
   Size:         20     811
 
BbvCI CC'TCA_GC

Cuts at:      0     341     831 
   Size:        341     490
 
BclI T'GATC_A

Cuts at:      0      99     831 
   Size:         99     732
 
BfrBI ATG'CAT

Cuts at:      0      67     831 
   Size:         67     764
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     447     831 
   Size:        447     384
 
Bpu10I CC'TnA_GC

Cuts at:      0     341     831 
   Size:        341     490
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     250     280     831 
   Size:        250      30     551

  Fragments arranged by size: 

                 551     250      30
 
BseYI C'CCAG_C

Cuts at:      0     742     831 
   Size:        742      89
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     316     831 
   Size:        316     515
 
BstBI TT'CG_AA

Cuts at:      0     669     831 
   Size:        669     162
 
BstEII G'GTnAC_C

Cuts at:      0     426     831 
   Size:        426     405
 
Bsu36I CC'TnA_GG

Cuts at:      0     541     831 
   Size:        541     290
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     695     831 
   Size:        695     136
 
BtsI GCAGTG_nn'

Cuts at:      0      74     117     831 
   Size:         74      43     714

  Fragments arranged by size: 

                 714      74      43
 
EcoO109I rG'GnC_Cy

Cuts at:      0     809     831 
   Size:        809      22
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     598     831 
   Size:        598     233
 
HaeII r_GCGC'y

Cuts at:      0     277     831 
   Size:        277     554
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     233     250     265     282     360     392     831 
   Size:        233      17      15      17      78      32     439

  Fragments arranged by size: 

                 439     233      78      32      17      17      15
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     831 
   Size:        236     595
 
MslI CAynn'nnrTG

Cuts at:      0     110     831 
   Size:        110     721
 
NsiI A_TGCA'T

Cuts at:      0      69     831 
   Size:         69     762
 
NspI r_CATG'y

Cuts at:      0      21     831 
   Size:         21     810
 
ScaI AGT'ACT

Cuts at:      0     446     831 
   Size:        446     385
 
StuI AGG'CCT

Cuts at:      0     527     831 
   Size:        527     304
 
StyI C'CwwG_G

Cuts at:      0     522     831 
   Size:        522     309
 
TatI w'GTAC_w

Cuts at:      0     444     831 
   Size:        444     387
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     648     831 
   Size:        648     183
 
XmnI GAAnn'nnTTC

Cuts at:      0     466     831 
   Size:        466     365
 

 Enzymes that do cut:

     AfeI     AloI    AlwNI     AvaI     BanI     BbsI    BbvCI     BclI
    BfrBI     BplI   Bpu10I    BsaXI    BseYI    BsmBI    BstBI   BstEII
   Bsu36I    BtgZI     BtsI EcoO109I     FalI    HaeII    Hin4I     MmeI
     MslI     NsiI     NspI     ScaI     StuI     StyI     TatI     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI     BcgI
    BciVI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BpmI    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BseRI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI Bsp1286I    BspEI    BspHI
    BspMI    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstXI    BstYI  BstZ17I     BtgI     ClaI    CspCI     DraI   DraIII
     DrdI     EaeI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI
    EcoRI    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI   MspA1I     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     PacI     PasI
     PciI    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI
     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     SwaI    TaqII     TstI  Tth111I     XbaI
     XhoI     XmaI     ZraI