(Linear) (Six-base) MAPSORT of: KIEE0441.seq  Check: 5031  from: 1  to: 699



KIEE0441.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:14  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581     699 
   Size:        581     118
 
AleI CACnn'nnGTG

Cuts at:      0      36     699 
   Size:         36     663
 
ApoI r'AATT_y

Cuts at:      0     256     699 
   Size:        256     443
 
BamHI G'GATC_C

Cuts at:      0     513     699 
   Size:        513     186
 
BanI G'GyrC_C

Cuts at:      0     483     699 
   Size:        483     216
 
BanII G_rGCy'C

Cuts at:      0     188     508     699 
   Size:        188     320     191

  Fragments arranged by size: 

                 320     191     188
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     481     699 
   Size:        481     218
 
BglI GCCn_nnn'nGGC

Cuts at:      0      13     699 
   Size:         13     686
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     202     699 
   Size:        202     497
 
Bpu10I CC'TnA_GC

Cuts at:      0     412     699 
   Size:        412     287
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     172     699 
   Size:        172     527
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     217     643     699 
   Size:        217     426      56

  Fragments arranged by size: 

                 426     217      56
 
BsiHKAI G_wGCw'C

Cuts at:      0     188     357     456     465     564     699 
   Size:        188     169      99       9      99     135

  Fragments arranged by size: 

                 188     169     135      99      99       9
 
Bsp1286I G_dGCh'C

Cuts at:      0     188     357     456     465     508     564     699 
   Size:        188     169      99       9      43      56     135

  Fragments arranged by size: 

                 188     169     135      99      56      43       9
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     544     699 
   Size:        544     155
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     460     699 
   Size:        460     239
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     426     699 
   Size:        426     273
 
BstYI r'GATC_y

Cuts at:      0     222     513     699 
   Size:        222     291     186

  Fragments arranged by size: 

                 291     222     186
 
Bsu36I CC'TnA_GG

Cuts at:      0     606     699 
   Size:        606      93
 
DraIII CAC_nnn'GTG

Cuts at:      0     460     666     699 
   Size:        460     206      33

  Fragments arranged by size: 

                 460     206      33
 
EaeI y'GGCC_r

Cuts at:      0       5     699 
   Size:          5     694
 
EarI CTCTTCn'nnn_

Cuts at:      0     176     345     470     699 
   Size:        176     169     125     229

  Fragments arranged by size: 

                 229     176     169     125
 
EcoICRI GAG'CTC

Cuts at:      0     186     699 
   Size:        186     513
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     581     699 
   Size:        581     118
 
EcoO109I rG'GnC_Cy

Cuts at:      0     150     533     699 
   Size:        150     383     166

  Fragments arranged by size: 

                 383     166     150
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     207     239     699 
   Size:        207      32     460

  Fragments arranged by size: 

                 460     207      32
 
HindIII A'AGCT_T

Cuts at:      0     337     699 
   Size:        337     362
 
MslI CAynn'nnrTG

Cuts at:      0      36     527     699 
   Size:         36     491     172

  Fragments arranged by size: 

                 491     172      36
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     395     427     699 
   Size:        395      32     272

  Fragments arranged by size: 

                 395     272      32
 
PpuMI rG'GwC_Cy

Cuts at:      0     150     533     699 
   Size:        150     383     166

  Fragments arranged by size: 

                 383     166     150
 
SacI G_AGCT'C

Cuts at:      0     188     699 
   Size:        188     511
 
SapI GCTCTTCn'nnn_

Cuts at:      0     176     345     470     699 
   Size:        176     169     125     229

  Fragments arranged by size: 

                 229     176     169     125
 
SmlI C'TyrA_G

Cuts at:      0     187     699 
   Size:        187     512
 
StuI AGG'CCT

Cuts at:      0     604     611     699 
   Size:        604       7      88

  Fragments arranged by size: 

                 604      88       7
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     658     699 
   Size:        658      41
 

 Enzymes that do cut:

     AcuI     AleI     ApoI    BamHI     BanI    BanII     BbsI     BglI
     BplI   Bpu10I    BpuEI    BseRI  BsiHKAI Bsp1286I    BspMI   BstAPI
    BstXI    BstYI   Bsu36I   DraIII     EaeI     EarI  EcoICRI  Eco57MI
 EcoO109I    Hin4I  HindIII     MslI     PpiI    PpuMI     SacI     SapI
     SmlI     StuI    TaqII

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI     BmrI
     BmtI     BpmI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI
    BseYI     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI     DrdI
     EagI     EciI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII     HpaI     KasI     KpnI     MfeI     MluI
     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII    SacII     SalI    SanDI
     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI
     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StyI     SwaI
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI