Restriction map for
pF1KE0422
(Linear) (Six-base) MAPSORT of: KIEE0422.seq Check: 7621 from: 1 to: 1179
KIEE0422.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AatII G_ACGT'C
Cuts at: 0 1136 1179
Size: 1136 43
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 213 361 586 1139 1179
Size: 28 185 148 225 553 40
Fragments arranged by size:
553 225 185 148 40 28
AleI CACnn'nnGTG
Cuts at: 0 239 1179
Size: 239 940
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1046 1179
Size: 1046 133
ApaI G_GGCC'C
Cuts at: 0 871 1179
Size: 871 308
ApaLI G'TGCA_C
Cuts at: 0 265 655 1179
Size: 265 390 524
Fragments arranged by size:
524 390 265
AvaI C'yCGr_G
Cuts at: 0 49 105 1179
Size: 49 56 1074
Fragments arranged by size:
1074 56 49
BanI G'GyrC_C
Cuts at: 0 88 1179
Size: 88 1091
BanII G_rGCy'C
Cuts at: 0 206 871 1179
Size: 206 665 308
Fragments arranged by size:
665 308 206
BbeI G_GCGC'C
Cuts at: 0 92 1179
Size: 92 1087
BbsI GAAGACnn'nnnn_
Cuts at: 0 219 1179
Size: 219 960
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 61 370 404 930 964 1179
Size: 27 34 309 34 526 34 215
Fragments arranged by size:
526 309 215 34 34 34 27
BglI GCCn_nnn'nGGC
Cuts at: 0 40 313 455 1174 1179
Size: 40 273 142 719 5
Fragments arranged by size:
719 273 142 40 5
Bme1580I G_kGCm'C
Cuts at: 0 269 659 871 1111 1179
Size: 269 390 212 240 68
Fragments arranged by size:
390 269 240 212 68
BmrI ACTGGGnnnn_n'
Cuts at: 0 688 1179
Size: 688 491
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 120 1179
Size: 120 1059
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 550 682 1179
Size: 550 132 497
Fragments arranged by size:
550 497 132
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1000 1179
Size: 1000 179
BsaAI yAC'GTr
Cuts at: 0 270 1179
Size: 270 909
BsaHI Gr'CG_yC
Cuts at: 0 89 1133 1179
Size: 89 1044 46
Fragments arranged by size:
1044 89 46
BsaWI w'CCGG_w
Cuts at: 0 1089 1179
Size: 1089 90
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 120 150 1179
Size: 120 30 1029
Fragments arranged by size:
1029 120 30
BseYI C'CCAG_C
Cuts at: 0 791 883 1179
Size: 791 92 296
Fragments arranged by size:
791 296 92
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1006 1179
Size: 1006 173
BsiEI CG_ry'CG
Cuts at: 0 20 653 1164 1179
Size: 20 633 511 15
Fragments arranged by size:
633 511 20 15
BsiHKAI G_wGCw'C
Cuts at: 0 50 206 269 659 1179
Size: 50 156 63 390 520
Fragments arranged by size:
520 390 156 63 50
Bsp1286I G_dGCh'C
Cuts at: 0 50 206 269 659 871 1111 1179
Size: 50 156 63 390 212 240 68
Fragments arranged by size:
390 240 212 156 68 63 50
BspEI T'CCGG_A
Cuts at: 0 1089 1179
Size: 1089 90
BspHI T'CATG_A
Cuts at: 0 671 1179
Size: 671 508
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 377 1179
Size: 377 802
BsrBI CCG'CTC
Cuts at: 0 632 1179
Size: 632 547
BsrDI GCAATG_nn'
Cuts at: 0 309 596 1179
Size: 309 287 583
Fragments arranged by size:
583 309 287
BsrFI r'CCGG_y
Cuts at: 0 25 937 1179
Size: 25 912 242
Fragments arranged by size:
912 242 25
BssSI C'ACGA_G
Cuts at: 0 94 1179
Size: 94 1085
BstEII G'GTnAC_C
Cuts at: 0 168 462 1179
Size: 168 294 717
Fragments arranged by size:
717 294 168
BstXI CCAn_nnnn'nTGG
Cuts at: 0 777 987 1179
Size: 777 210 192
Fragments arranged by size:
777 210 192
BtgI C'CryG_G
Cuts at: 0 974 1064 1169 1179
Size: 974 90 105 10
Fragments arranged by size:
974 105 90 10
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 698 948 1179
Size: 698 250 231
Fragments arranged by size:
698 250 231
DraIII CAC_nnn'GTG
Cuts at: 0 168 1179
Size: 168 1011
EaeI y'GGCC_r
Cuts at: 0 314 650 897 977 989 1161 1179
Size: 314 336 247 80 12 172 18
Fragments arranged by size:
336 314 247 172 80 18 12
EagI C'GGCC_G
Cuts at: 0 650 1161 1179
Size: 650 511 18
Fragments arranged by size:
650 511 18
EarI CTCTTCn'nnn_
Cuts at: 0 254 284 1179
Size: 254 30 895
Fragments arranged by size:
895 254 30
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 310 1129 1179
Size: 310 819 50
Fragments arranged by size:
819 310 50
EcoICRI GAG'CTC
Cuts at: 0 204 1179
Size: 204 975
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 28 213 361 550 586 682 1139 1179
Size: 28 185 148 189 36 96 457 40
Fragments arranged by size:
457 189 185 148 96 40 36 28
EcoO109I rG'GnC_Cy
Cuts at: 0 99 867 1179
Size: 99 768 312
Fragments arranged by size:
768 312 99
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 243 1179
Size: 243 936
FspI TGC'GCA
Cuts at: 0 595 1179
Size: 595 584
HaeII r_GCGC'y
Cuts at: 0 7 35 92 1179
Size: 7 28 57 1087
Fragments arranged by size:
1087 57 28 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 804 836 1179
Size: 804 32 343
Fragments arranged by size:
804 343 32
HincII GTy'rAC
Cuts at: 0 840 1179
Size: 840 339
KasI G'GCGC_C
Cuts at: 0 88 1179
Size: 88 1091
MscI TGG'CCA
Cuts at: 0 316 899 991 1179
Size: 316 583 92 188
Fragments arranged by size:
583 316 188 92
MslI CAynn'nnrTG
Cuts at: 0 239 539 754 769 811 1028 1179
Size: 239 300 215 15 42 217 151
Fragments arranged by size:
300 239 217 215 151 42 15
MspA1I CmG'CkG
Cuts at: 0 777 995 1179
Size: 777 218 184
Fragments arranged by size:
777 218 184
NaeI GCC'GGC
Cuts at: 0 27 1179
Size: 27 1152
NarI GG'CG_CC
Cuts at: 0 89 1179
Size: 89 1090
NcoI C'CATG_G
Cuts at: 0 1064 1179
Size: 1064 115
NgoMIV G'CCGG_C
Cuts at: 0 25 1179
Size: 25 1154
NspI r_CATG'y
Cuts at: 0 217 242 1179
Size: 217 25 937
Fragments arranged by size:
937 217 25
PasI CC'CwG_GG
Cuts at: 0 74 181 1179
Size: 74 107 998
Fragments arranged by size:
998 107 74
PflMI CCAn_nnn'nTGG
Cuts at: 0 187 1179
Size: 187 992
PfoI T'CCnGG_A
Cuts at: 0 497 1127 1179
Size: 497 630 52
Fragments arranged by size:
630 497 52
PmlI CAC'GTG
Cuts at: 0 270 1179
Size: 270 909
PpuMI rG'GwC_Cy
Cuts at: 0 99 1179
Size: 99 1080
PspOMI G'GGCC_C
Cuts at: 0 867 1179
Size: 867 312
PspXI vC'TCGA_Gb
Cuts at: 0 105 1179
Size: 105 1074
PstI C_TGCA'G
Cuts at: 0 709 1179
Size: 709 470
PvuII CAG'CTG
Cuts at: 0 777 995 1179
Size: 777 218 184
Fragments arranged by size:
777 218 184
SacI G_AGCT'C
Cuts at: 0 206 1179
Size: 206 973
SfcI C'TryA_G
Cuts at: 0 705 1179
Size: 705 474
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1174 1179
Size: 1174 5
SfoI GGC'GCC
Cuts at: 0 90 1179
Size: 90 1089
SmlI C'TyrA_G
Cuts at: 0 105 1015 1179
Size: 105 910 164
Fragments arranged by size:
910 164 105
StuI AGG'CCT
Cuts at: 0 875 1179
Size: 875 304
StyI C'CwwG_G
Cuts at: 0 578 1064 1179
Size: 578 486 115
Fragments arranged by size:
578 486 115
TatI w'GTAC_w
Cuts at: 0 518 1179
Size: 518 661
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 259 291 1179
Size: 259 32 888
Fragments arranged by size:
888 259 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 774 1179
Size: 774 405
XhoI C'TCGA_G
Cuts at: 0 105 1179
Size: 105 1074
ZraI GAC'GTC
Cuts at: 0 1134 1179
Size: 1134 45
Enzymes that do cut:
AatII AcuI AleI AlfI ApaI ApaLI AvaI BanI
BanII BbeI BbsI BcgI BglI Bme1580I BmrI BplI
BpmI BpuEI BsaAI BsaHI BsaWI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI
BsrFI BssSI BstEII BstXI BtgI BtgZI DraIII EaeI
EagI EarI EciI EcoICRI Eco57MI EcoO109I FalI FspI
HaeII Hin4I HincII KasI MscI MslI MspA1I NaeI
NarI NcoI NgoMIV NspI PasI PflMI PfoI PmlI
PpuMI PspOMI PspXI PstI PvuII SacI SfcI SfiI
SfoI SmlI StuI StyI TatI TstI XcmI XhoI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AloI AlwNI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbvCI BciVI BclI BfrBI BglII BlpI
BmgBI BmtI Bpu10I BsaI BsaBI BseRI BsiWI BsmI
BsmBI BsrGI BssHII BstAPI BstBI BstYI BstZ17I Bsu36I
BtsI ClaI CspCI DraI DrdI EcoNI EcoRI EcoRV
FseI FspAI HindIII HpaI KpnI MfeI MluI MmeI
NdeI NheI NotI NruI NsiI PacI PciI PmeI
PpiI PshAI PsiI PsrI PvuI RsrII SacII SalI
SanDI SapI SbfI ScaI SexAI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TaqII Tth111I
XbaI XmaI XmnI