Restriction map for
pF1KE0421
(Linear) (Six-base) MAPSORT of: KIEE0421.seq Check: 9032 from: 1 to: 567
KIEE0421.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 340 457 567
Size: 212 128 117 110
Fragments arranged by size:
212 128 117 110
AflIII A'CryG_T
Cuts at: 0 84 567
Size: 84 483
ApaLI G'TGCA_C
Cuts at: 0 285 567
Size: 285 282
ApoI r'AATT_y
Cuts at: 0 147 172 567
Size: 147 25 395
Fragments arranged by size:
395 147 25
BbvCI CC'TCA_GC
Cuts at: 0 485 567
Size: 485 82
BglI GCCn_nnn'nGGC
Cuts at: 0 195 567
Size: 195 372
Bme1580I G_kGCm'C
Cuts at: 0 289 567
Size: 289 278
BmrI ACTGGGnnnn_n'
Cuts at: 0 383 536 567
Size: 383 153 31
Fragments arranged by size:
383 153 31
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 121 567
Size: 121 446
Bpu10I CC'TnA_GC
Cuts at: 0 485 567
Size: 485 82
BsaAI yAC'GTr
Cuts at: 0 19 85 567
Size: 19 66 482
Fragments arranged by size:
482 66 19
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 481 511 567
Size: 481 30 56
Fragments arranged by size:
481 56 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 502 567
Size: 502 65
BseYI C'CCAG_C
Cuts at: 0 254 567
Size: 254 313
BsiHKAI G_wGCw'C
Cuts at: 0 289 567
Size: 289 278
Bsp1286I G_dGCh'C
Cuts at: 0 289 567
Size: 289 278
BssHII G'CGCG_C
Cuts at: 0 49 567
Size: 49 518
BstXI CCAn_nnnn'nTGG
Cuts at: 0 310 567
Size: 310 257
BstYI r'GATC_y
Cuts at: 0 75 440 567
Size: 75 365 127
Fragments arranged by size:
365 127 75
BtgI C'CryG_G
Cuts at: 0 59 190 567
Size: 59 131 377
Fragments arranged by size:
377 131 59
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 314 349 567
Size: 314 35 218
Fragments arranged by size:
314 218 35
EaeI y'GGCC_r
Cuts at: 0 116 567
Size: 116 451
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 212 340 457 567
Size: 212 128 117 110
Fragments arranged by size:
212 128 117 110
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 335 367 481 513 567
Size: 335 32 114 32 54
Fragments arranged by size:
335 114 54 32 32
HincII GTy'rAC
Cuts at: 0 390 567
Size: 390 177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 567
Size: 291 276
MslI CAynn'nnrTG
Cuts at: 0 112 567
Size: 112 455
MspA1I CmG'CkG
Cuts at: 0 61 258 331 435 567
Size: 61 197 73 104 132
Fragments arranged by size:
197 132 104 73 61
NcoI C'CATG_G
Cuts at: 0 190 567
Size: 190 377
NruI TCG'CGA
Cuts at: 0 96 567
Size: 96 471
PflMI CCAn_nnn'nTGG
Cuts at: 0 36 567
Size: 36 531
PmlI CAC'GTG
Cuts at: 0 19 567
Size: 19 548
PvuII CAG'CTG
Cuts at: 0 258 331 435 567
Size: 258 73 104 132
Fragments arranged by size:
258 132 104 73
SacII CC_GC'GG
Cuts at: 0 62 567
Size: 62 505
StyI C'CwwG_G
Cuts at: 0 190 567
Size: 190 377
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 17 49 567
Size: 17 32 518
Fragments arranged by size:
518 32 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 262 567
Size: 262 305
Enzymes that do cut:
AcuI AflIII ApaLI ApoI BbvCI BglI Bme1580I BmrI
BplI Bpu10I BsaAI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BssHII BstXI BstYI BtgI CspCI EaeI Eco57MI Hin4I
HincII MmeI MslI MspA1I NcoI NruI PflMI PmlI
PvuII SacII StyI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI AloI AlwNI ApaI AscI AseI
AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI
BbsI BcgI BciVI BclI BfrBI BglII BlpI BmgBI
BmtI BpmI BpuEI BsaI BsaBI BsaHI BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssSI BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI DraI DraIII DrdI EagI
EarI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI
NotI NsiI NspI PacI PasI PciI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PspXI PsrI PstI
PvuI RsrII SacI SalI SanDI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII
TatI Tth111I XbaI XhoI XmaI XmnI ZraI