Restriction map for
pF1KE0414
(Linear) (Six-base) MAPSORT of: KIEE0414.seq Check: 9873 from: 1 to: 558
KIEE0414.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 558
Size: 412 146
AfeI AGC'GCT
Cuts at: 0 295 558
Size: 295 263
AflIII A'CryG_T
Cuts at: 0 167 558
Size: 167 391
ApaLI G'TGCA_C
Cuts at: 0 346 558
Size: 346 212
AvaI C'yCGr_G
Cuts at: 0 174 217 558
Size: 174 43 341
Fragments arranged by size:
341 174 43
BanI G'GyrC_C
Cuts at: 0 100 468 558
Size: 100 368 90
Fragments arranged by size:
368 100 90
BanII G_rGCy'C
Cuts at: 0 524 558
Size: 524 34
BbeI G_GCGC'C
Cuts at: 0 104 558
Size: 104 454
Bme1580I G_kGCm'C
Cuts at: 0 350 473 558
Size: 350 123 85
Fragments arranged by size:
350 123 85
BmgBI CAC'GTC
Cuts at: 0 345 465 558
Size: 345 120 93
Fragments arranged by size:
345 120 93
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 252 558
Size: 252 306
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 558
Size: 340 218
Bpu10I CC'TnA_GC
Cuts at: 0 307 558
Size: 307 251
BsaAI yAC'GTr
Cuts at: 0 168 558
Size: 168 390
BsaHI Gr'CG_yC
Cuts at: 0 101 558
Size: 101 457
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 78 558
Size: 78 480
BseYI C'CCAG_C
Cuts at: 0 327 558
Size: 327 231
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 409 558
Size: 409 149
BsiEI CG_ry'CG
Cuts at: 0 440 558
Size: 440 118
BsiHKAI G_wGCw'C
Cuts at: 0 350 524 558
Size: 350 174 34
Fragments arranged by size:
350 174 34
Bsp1286I G_dGCh'C
Cuts at: 0 350 473 524 558
Size: 350 123 51 34
Fragments arranged by size:
350 123 51 34
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 416 558
Size: 416 142
BssHII G'CGCG_C
Cuts at: 0 115 311 313 411 558
Size: 115 196 2 98 147
Fragments arranged by size:
196 147 115 98 2
BstXI CCAn_nnnn'nTGG
Cuts at: 0 249 483 558
Size: 249 234 75
Fragments arranged by size:
249 234 75
BstYI r'GATC_y
Cuts at: 0 83 558
Size: 83 475
BtgI C'CryG_G
Cuts at: 0 260 558
Size: 260 298
EaeI y'GGCC_r
Cuts at: 0 206 437 558
Size: 206 231 121
Fragments arranged by size:
231 206 121
EagI C'GGCC_G
Cuts at: 0 437 558
Size: 437 121
EarI CTCTTCn'nnn_
Cuts at: 0 553 558
Size: 553 5
EcoICRI GAG'CTC
Cuts at: 0 522 558
Size: 522 36
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 412 558
Size: 340 72 146
Fragments arranged by size:
340 146 72
FspI TGC'GCA
Cuts at: 0 448 558
Size: 448 110
HaeII r_GCGC'y
Cuts at: 0 104 297 558
Size: 104 193 261
Fragments arranged by size:
261 193 104
KasI G'GCGC_C
Cuts at: 0 100 558
Size: 100 458
MscI TGG'CCA
Cuts at: 0 208 558
Size: 208 350
MslI CAynn'nnrTG
Cuts at: 0 463 558
Size: 463 95
MspA1I CmG'CkG
Cuts at: 0 144 399 558
Size: 144 255 159
Fragments arranged by size:
255 159 144
NarI GG'CG_CC
Cuts at: 0 101 558
Size: 101 457
PvuII CAG'CTG
Cuts at: 0 144 558
Size: 144 414
SacI G_AGCT'C
Cuts at: 0 524 558
Size: 524 34
SfcI C'TryA_G
Cuts at: 0 333 558
Size: 333 225
SfoI GGC'GCC
Cuts at: 0 102 558
Size: 102 456
SmaI CCC'GGG
Cuts at: 0 176 558
Size: 176 382
StyI C'CwwG_G
Cuts at: 0 527 558
Size: 527 31
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 246 558
Size: 246 312
XmaI C'CCGG_G
Cuts at: 0 174 558
Size: 174 384
Enzymes that do cut:
AcuI AfeI AflIII ApaLI AvaI BanI BanII BbeI
Bme1580I BmgBI BplI BpmI Bpu10I BsaAI BsaHI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspMI BssHII BstXI
BstYI BtgI EaeI EagI EarI EcoICRI Eco57MI FspI
HaeII KasI MscI MslI MspA1I NarI PvuII SacI
SfcI SfoI SmaI StyI XcmI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AhdI
AleI AlfI AloI AlwNI ApaI ApoI AscI AseI
AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI
BclI BfrBI BglI BglII BlpI BmrI BmtI BpuEI
BsaI BsaBI BsaWI BsaXI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EciI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspAI Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TaqII TatI
TstI Tth111I XbaI XhoI XmnI ZraI