Restriction map for
pF1KE0409
(Linear) (Six-base) MAPSORT of: KIEE0409.seq Check: 6252 from: 1 to: 585
KIEE0409.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AccI GT'mk_AC
Cuts at: 0 192 585
Size: 192 393
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 473 585
Size: 143 330 112
Fragments arranged by size:
330 143 112
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 145 177 585
Size: 145 32 408
Fragments arranged by size:
408 145 32
AlwNI CAG_nnn'CTG
Cuts at: 0 358 585
Size: 358 227
ApaI G_GGCC'C
Cuts at: 0 234 585
Size: 234 351
ApoI r'AATT_y
Cuts at: 0 107 585
Size: 107 478
BanI G'GyrC_C
Cuts at: 0 573 585
Size: 573 12
BanII G_rGCy'C
Cuts at: 0 26 234 585
Size: 26 208 351
Fragments arranged by size:
351 208 26
BbsI GAAGACnn'nnnn_
Cuts at: 0 516 585
Size: 516 69
BbvCI CC'TCA_GC
Cuts at: 0 343 585
Size: 343 242
BlpI GC'TnA_GC
Cuts at: 0 73 585
Size: 73 512
Bme1580I G_kGCm'C
Cuts at: 0 234 585
Size: 234 351
BmrI ACTGGGnnnn_n'
Cuts at: 0 228 585
Size: 228 357
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 42 585
Size: 42 543
Bpu10I CC'TnA_GC
Cuts at: 0 343 585
Size: 343 242
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 553 585
Size: 283 270 32
Fragments arranged by size:
283 270 32
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 34 585
Size: 34 551
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 34 585
Size: 34 551
BsiHKAI G_wGCw'C
Cuts at: 0 26 585
Size: 26 559
Bsp1286I G_dGCh'C
Cuts at: 0 26 234 585
Size: 26 208 351
Fragments arranged by size:
351 208 26
BspHI T'CATG_A
Cuts at: 0 300 585
Size: 300 285
BsrBI CCG'CTC
Cuts at: 0 80 585
Size: 80 505
BssSI C'ACGA_G
Cuts at: 0 58 585
Size: 58 527
BtgI C'CryG_G
Cuts at: 0 401 585
Size: 401 184
BtsI GCAGTG_nn'
Cuts at: 0 369 375 585
Size: 369 6 210
Fragments arranged by size:
369 210 6
EaeI y'GGCC_r
Cuts at: 0 35 585
Size: 35 550
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 19 585
Size: 19 566
EcoICRI GAG'CTC
Cuts at: 0 24 585
Size: 24 561
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 143 473 585
Size: 143 330 112
Fragments arranged by size:
330 143 112
EcoO109I rG'GnC_Cy
Cuts at: 0 53 230 585
Size: 53 177 355
Fragments arranged by size:
355 177 53
EcoRI G'AATT_C
Cuts at: 0 107 585
Size: 107 478
HincII GTy'rAC
Cuts at: 0 224 585
Size: 224 361
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 266 585
Size: 266 319
MslI CAynn'nnrTG
Cuts at: 0 400 585
Size: 400 185
NcoI C'CATG_G
Cuts at: 0 401 585
Size: 401 184
PflMI CCAn_nnn'nTGG
Cuts at: 0 358 585
Size: 358 227
PspOMI G'GGCC_C
Cuts at: 0 230 585
Size: 230 355
PstI C_TGCA'G
Cuts at: 0 52 585
Size: 52 533
SacI G_AGCT'C
Cuts at: 0 26 585
Size: 26 559
SexAI A'CCwGG_T
Cuts at: 0 195 585
Size: 195 390
SfcI C'TryA_G
Cuts at: 0 48 547 585
Size: 48 499 38
Fragments arranged by size:
499 48 38
SmlI C'TyrA_G
Cuts at: 0 262 568 585
Size: 262 306 17
Fragments arranged by size:
306 262 17
StyI C'CwwG_G
Cuts at: 0 401 585
Size: 401 184
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 18 585
Size: 18 567
TatI w'GTAC_w
Cuts at: 0 379 585
Size: 379 206
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 355 585
Size: 355 230
Enzymes that do cut:
AccI AcuI AloI AlwNI ApaI ApoI BanI BanII
BbsI BbvCI BlpI Bme1580I BmrI BplI Bpu10I BpuEI
BseRI BsgI BsiHKAI Bsp1286I BspHI BsrBI BssSI BtgI
BtsI EaeI EciI EcoICRI Eco57MI EcoO109I EcoRI HincII
MmeI MslI NcoI PflMI PspOMI PstI SacI SexAI
SfcI SmlI StyI TaqII TatI XcmI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BcgI BciVI BclI BfrBI
BglI BglII BmgBI BmtI BpmI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI
BspEI BspMI BsrDI BsrFI BsrGI BssHII BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EarI EcoNI EcoRV FalI
FseI FspI FspAI HaeII Hin4I HindIII HpaI KasI
KpnI MfeI MluI MscI MspA1I NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacII SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI