Restriction map for
pF1KE0404
(Linear) (Six-base) MAPSORT of: KIEE0404.seq Check: 4005 from: 1 to: 822
KIEE0404.orf
With 182 enzymes: SgfI *
April 24, 2009 13:14 ..
AatII G_ACGT'C
Cuts at: 0 529 822
Size: 529 293
AflIII A'CryG_T
Cuts at: 0 54 822
Size: 54 768
AhdI GACnn_n'nnGTC
Cuts at: 0 489 822
Size: 489 333
AlwNI CAG_nnn'CTG
Cuts at: 0 615 696 822
Size: 615 81 126
Fragments arranged by size:
615 126 81
ApoI r'AATT_y
Cuts at: 0 190 451 822
Size: 190 261 371
Fragments arranged by size:
371 261 190
BanII G_rGCy'C
Cuts at: 0 347 822
Size: 347 475
BbvCI CC'TCA_GC
Cuts at: 0 679 822
Size: 679 143
BlpI GC'TnA_GC
Cuts at: 0 47 822
Size: 47 775
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 361 601 822
Size: 267 94 240 221
Fragments arranged by size:
267 240 221 94
Bpu10I CC'TnA_GC
Cuts at: 0 679 822
Size: 679 143
BsaHI Gr'CG_yC
Cuts at: 0 526 595 822
Size: 526 69 227
Fragments arranged by size:
526 227 69
BsaWI w'CCGG_w
Cuts at: 0 9 27 822
Size: 9 18 795
Fragments arranged by size:
795 18 9
BseYI C'CCAG_C
Cuts at: 0 657 792 822
Size: 657 135 30
Fragments arranged by size:
657 135 30
BsiHKAI G_wGCw'C
Cuts at: 0 347 374 822
Size: 347 27 448
Fragments arranged by size:
448 347 27
Bsp1286I G_dGCh'C
Cuts at: 0 347 374 822
Size: 347 27 448
Fragments arranged by size:
448 347 27
BspEI T'CCGG_A
Cuts at: 0 9 822
Size: 9 813
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 494 564 822
Size: 494 70 258
Fragments arranged by size:
494 258 70
BsrDI GCAATG_nn'
Cuts at: 0 690 803 822
Size: 690 113 19
Fragments arranged by size:
690 113 19
BsrFI r'CCGG_y
Cuts at: 0 571 822
Size: 571 251
BstYI r'GATC_y
Cuts at: 0 431 777 822
Size: 431 346 45
Fragments arranged by size:
431 346 45
BtgI C'CryG_G
Cuts at: 0 386 735 822
Size: 386 349 87
Fragments arranged by size:
386 349 87
BtsI GCAGTG_nn'
Cuts at: 0 86 822
Size: 86 736
EarI CTCTTCn'nnn_
Cuts at: 0 800 822
Size: 800 22
EcoICRI GAG'CTC
Cuts at: 0 345 822
Size: 345 477
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 267 361 601 822
Size: 267 94 240 221
Fragments arranged by size:
267 240 221 94
EcoO109I rG'GnC_Cy
Cuts at: 0 483 675 822
Size: 483 192 147
Fragments arranged by size:
483 192 147
EcoRI G'AATT_C
Cuts at: 0 190 451 822
Size: 190 261 371
Fragments arranged by size:
371 261 190
HaeII r_GCGC'y
Cuts at: 0 18 822
Size: 18 804
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 44 76 475 507 822
Size: 44 32 399 32 315
Fragments arranged by size:
399 315 44 32 32
MslI CAynn'nnrTG
Cuts at: 0 67 822
Size: 67 755
MspA1I CmG'CkG
Cuts at: 0 90 339 647 822
Size: 90 249 308 175
Fragments arranged by size:
308 249 175 90
NaeI GCC'GGC
Cuts at: 0 573 822
Size: 573 249
NcoI C'CATG_G
Cuts at: 0 386 822
Size: 386 436
NgoMIV G'CCGG_C
Cuts at: 0 571 822
Size: 571 251
NspI r_CATG'y
Cuts at: 0 58 822
Size: 58 764
PciI A'CATG_T
Cuts at: 0 54 822
Size: 54 768
PflMI CCAn_nnn'nTGG
Cuts at: 0 615 822
Size: 615 207
PfoI T'CCnGG_A
Cuts at: 0 455 822
Size: 455 367
PpuMI rG'GwC_Cy
Cuts at: 0 483 822
Size: 483 339
PvuII CAG'CTG
Cuts at: 0 90 647 822
Size: 90 557 175
Fragments arranged by size:
557 175 90
SacI G_AGCT'C
Cuts at: 0 347 822
Size: 347 475
SapI GCTCTTCn'nnn_
Cuts at: 0 800 822
Size: 800 22
SspI AAT'ATT
Cuts at: 0 148 822
Size: 148 674
StyI C'CwwG_G
Cuts at: 0 386 560 822
Size: 386 174 262
Fragments arranged by size:
386 262 174
TatI w'GTAC_w
Cuts at: 0 641 822
Size: 641 181
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 67 99 610 642 822
Size: 67 32 511 32 180
Fragments arranged by size:
511 180 67 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 528 822
Size: 528 294
ZraI GAC'GTC
Cuts at: 0 527 822
Size: 527 295
Enzymes that do cut:
AatII AflIII AhdI AlwNI ApoI BanII BbvCI BlpI
BpmI Bpu10I BsaHI BsaWI BseYI BsiHKAI Bsp1286I BspEI
BspMI BsrDI BsrFI BstYI BtgI BtsI EarI EcoICRI
Eco57MI EcoO109I EcoRI HaeII Hin4I MslI MspA1I NaeI
NcoI NgoMIV NspI PciI PflMI PfoI PpuMI PvuII
SacI SapI SspI StyI TatI TstI Tth111I ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AleI AlfI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BamHI BanI BbeI BbsI BcgI
BciVI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI
BmtI BplI BpuEI BsaI BsaAI BsaBI BsaXI BseRI
BsgI BsiEI BsiWI BsmI BsmBI BspHI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI
EciI EcoNI EcoRV FalI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
NarI NdeI NheI NotI NruI NsiI PacI PasI
PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI StuI SwaI TaqII XbaI
XcmI XhoI XmaI XmnI