Restriction map for
pF1KE0385
(Linear) (Six-base) MAPSORT of: KIEE0385.seq Check: 481 from: 1 to: 2295
KIEE0385.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 686 2295
Size: 686 1609
Acc65I G'GTAC_C
Cuts at: 0 1114 2295
Size: 1114 1181
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 557 1157 2295
Size: 557 600 1138
Fragments arranged by size:
1138 600 557
AfeI AGC'GCT
Cuts at: 0 78 2295
Size: 78 2217
AflIII A'CryG_T
Cuts at: 0 1023 1559 1782 2295
Size: 1023 536 223 513
Fragments arranged by size:
1023 536 513 223
AhdI GACnn_n'nnGTC
Cuts at: 0 1322 2295
Size: 1322 973
AleI CACnn'nnGTG
Cuts at: 0 321 611 2295
Size: 321 290 1684
Fragments arranged by size:
1684 321 290
AlwNI CAG_nnn'CTG
Cuts at: 0 301 2295
Size: 301 1994
ApoI r'AATT_y
Cuts at: 0 118 951 1226 1454 2295
Size: 118 833 275 228 841
Fragments arranged by size:
841 833 275 228 118
AseI AT'TA_AT
Cuts at: 0 1472 1894 2017 2295
Size: 1472 422 123 278
Fragments arranged by size:
1472 422 278 123
AvrII C'CTAG_G
Cuts at: 0 1198 2295
Size: 1198 1097
BamHI G'GATC_C
Cuts at: 0 913 2295
Size: 913 1382
BanI G'GyrC_C
Cuts at: 0 1114 2295
Size: 1114 1181
BbsI GAAGACnn'nnnn_
Cuts at: 0 700 2295
Size: 700 1595
BciVI GTATCCnnnnn_n'
Cuts at: 0 1833 2295
Size: 1833 462
BclI T'GATC_A
Cuts at: 0 1674 2295
Size: 1674 621
BfrBI ATG'CAT
Cuts at: 0 1698 2295
Size: 1698 597
BglI GCCn_nnn'nGGC
Cuts at: 0 41 2295
Size: 41 2254
BglII A'GATC_T
Cuts at: 0 1253 2295
Size: 1253 1042
Bme1580I G_kGCm'C
Cuts at: 0 751 2295
Size: 751 1544
BmgBI CAC'GTC
Cuts at: 0 24 1026 2295
Size: 24 1002 1269
Fragments arranged by size:
1269 1002 24
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1260 2295
Size: 1260 1035
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 1055 1526 2171 2295
Size: 449 606 471 645 124
Fragments arranged by size:
645 606 471 449 124
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1252 1637 2295
Size: 1252 385 658
Fragments arranged by size:
1252 658 385
BsaI GGTCTCn'nnnn_
Cuts at: 0 2024 2295
Size: 2024 271
BsaAI yAC'GTr
Cuts at: 0 861 2295
Size: 861 1434
BsaBI GATnn'nnATC
Cuts at: 0 2019 2295
Size: 2019 276
BsaHI Gr'CG_yC
Cuts at: 0 2046 2295
Size: 2046 249
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 784 814 1028 1031 1058 1061 2295
Size: 784 30 214 3 27 3 1234
Fragments arranged by size:
1234 784 214 30 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 19 446 1052 1249 2295
Size: 19 427 606 197 1046
Fragments arranged by size:
1046 606 427 197 19
BseYI C'CCAG_C
Cuts at: 0 73 301 2295
Size: 73 228 1994
Fragments arranged by size:
1994 228 73
BsiEI CG_ry'CG
Cuts at: 0 1446 2295
Size: 1446 849
BsiHKAI G_wGCw'C
Cuts at: 0 380 558 906 939 1018 2295
Size: 380 178 348 33 79 1277
Fragments arranged by size:
1277 380 348 178 79 33
BsmI GAATG_Cn'
Cuts at: 0 1761 2295
Size: 1761 534
Bsp1286I G_dGCh'C
Cuts at: 0 380 558 751 906 939 1018 2295
Size: 380 178 193 155 33 79 1277
Fragments arranged by size:
1277 380 193 178 155 79 33
BspHI T'CATG_A
Cuts at: 0 546 842 2226 2295
Size: 546 296 1384 69
Fragments arranged by size:
1384 546 296 69
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2115 2295
Size: 2115 180
BsrDI GCAATG_nn'
Cuts at: 0 581 2295
Size: 581 1714
BsrFI r'CCGG_y
Cuts at: 0 31 60 2295
Size: 31 29 2235
Fragments arranged by size:
2235 31 29
BstBI TT'CG_AA
Cuts at: 0 143 2295
Size: 143 2152
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1326 1642 1720 2295
Size: 1326 316 78 575
Fragments arranged by size:
1326 575 316 78
BstYI r'GATC_y
Cuts at: 0 913 1253 1735 2295
Size: 913 340 482 560
Fragments arranged by size:
913 560 482 340
BtgI C'CryG_G
Cuts at: 0 751 1641 1713 1731 2295
Size: 751 890 72 18 564
Fragments arranged by size:
890 751 564 72 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1842 2295
Size: 1842 453
ClaI AT'CG_AT
Cuts at: 0 1192 2295
Size: 1192 1103
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 2156 2191 2295
Size: 2156 35 104
Fragments arranged by size:
2156 104 35
DraI TTT'AAA
Cuts at: 0 288 2295
Size: 288 2007
EaeI y'GGCC_r
Cuts at: 0 29 1443 2295
Size: 29 1414 852
Fragments arranged by size:
1414 852 29
EagI C'GGCC_G
Cuts at: 0 1443 2295
Size: 1443 852
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 557 1055 1157 1526 2171 2295
Size: 449 108 498 102 369 645 124
Fragments arranged by size:
645 498 449 369 124 108 102
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1135 2295
Size: 1135 1160
FseI GG_CCGG'CC
Cuts at: 0 35 2295
Size: 35 2260
HaeII r_GCGC'y
Cuts at: 0 80 2295
Size: 80 2215
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1308 1340 1524 1556 1841 1873 2295
Size: 1308 32 184 32 285 32 422
Fragments arranged by size:
1308 422 285 184 32 32 32
HincII GTy'rAC
Cuts at: 0 687 1565 2295
Size: 687 878 730
Fragments arranged by size:
878 730 687
HindIII A'AGCT_T
Cuts at: 0 713 2295
Size: 713 1582
HpaI GTT'AAC
Cuts at: 0 1565 2295
Size: 1565 730
KpnI G_GTAC'C
Cuts at: 0 1118 2295
Size: 1118 1177
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1560 2295
Size: 1560 735
MslI CAynn'nnrTG
Cuts at: 0 321 551 611 1558 1634 1640 1697 1718
Size: 321 230 60 947 76 6 57 21
Cuts at: 1718 1813 2295
Size: 95 482
Fragments arranged by size:
947 482 321 230 95 76 60 57
21 6
NaeI GCC'GGC
Cuts at: 0 33 2295
Size: 33 2262
NcoI C'CATG_G
Cuts at: 0 751 1641 1713 2295
Size: 751 890 72 582
Fragments arranged by size:
890 751 582 72
NdeI CA'TA_TG
Cuts at: 0 150 566 1694 2201 2295
Size: 150 416 1128 507 94
Fragments arranged by size:
1128 507 416 150 94
NgoMIV G'CCGG_C
Cuts at: 0 31 2295
Size: 31 2264
NsiI A_TGCA'T
Cuts at: 0 1700 2295
Size: 1700 595
NspI r_CATG'y
Cuts at: 0 295 1356 1563 1786 2295
Size: 295 1061 207 223 509
Fragments arranged by size:
1061 509 295 223 207
PciI A'CATG_T
Cuts at: 0 1559 1782 2295
Size: 1559 223 513
Fragments arranged by size:
1559 513 223
PfoI T'CCnGG_A
Cuts at: 0 848 2295
Size: 848 1447
PsiI TTA'TAA
Cuts at: 0 1154 2295
Size: 1154 1141
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1814 1846 2295
Size: 1814 32 449
Fragments arranged by size:
1814 449 32
SalI G'TCGA_C
Cuts at: 0 685 2295
Size: 685 1610
ScaI AGT'ACT
Cuts at: 0 632 2217 2295
Size: 632 1585 78
Fragments arranged by size:
1585 632 78
SmlI C'TyrA_G
Cuts at: 0 1231 1652 2295
Size: 1231 421 643
Fragments arranged by size:
1231 643 421
SspI AAT'ATT
Cuts at: 0 284 1898 2295
Size: 284 1614 397
Fragments arranged by size:
1614 397 284
StyI C'CwwG_G
Cuts at: 0 54 751 1198 1641 1713 2295
Size: 54 697 447 443 72 582
Fragments arranged by size:
697 582 447 443 72 54
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 293 1153 2295
Size: 293 860 1142
Fragments arranged by size:
1142 860 293
TatI w'GTAC_w
Cuts at: 0 630 2118 2215 2295
Size: 630 1488 97 80
Fragments arranged by size:
1488 630 97 80
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1031 1063 2295
Size: 1031 32 1232
Fragments arranged by size:
1232 1031 32
Enzymes that do cut:
AccI Acc65I AcuI AfeI AflIII AhdI AleI AlwNI
ApoI AseI AvrII BamHI BanI BbsI BciVI BclI
BfrBI BglI BglII Bme1580I BmgBI BplI BpmI BpuEI
BsaI BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BsrFI BstBI
BstXI BstYI BtgI BtgZI ClaI CspCI DraI EaeI
EagI Eco57MI FalI FseI HaeII Hin4I HincII HindIII
HpaI KpnI MmeI MslI NaeI NcoI NdeI NgoMIV
NsiI NspI PciI PfoI PsiI PsrI SalI ScaI
SmlI SspI StyI TaqII TatI TstI
Enzymes that do not cut:
AarI AatII AclI AflII AgeI AlfI AloI ApaI
ApaLI AscI AsiSI AvaI BaeI BanII BbeI BbvCI
BcgI BlpI BmrI BmtI Bpu10I BsaWI BsgI BsiWI
BsmBI BspEI BsrBI BsrGI BssHII BssSI BstAPI BstEII
BstZ17I Bsu36I BtsI DraIII DrdI EarI EciI EcoICRI
EcoNI EcoO109I EcoRI EcoRV FspI FspAI KasI MfeI
MluI MscI MspA1I NarI NheI NotI NruI PacI
PasI PflMI PmeI PmlI PpiI PpuMI PshAI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SanDI
SapI SbfI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI