Restriction map for
pF1KE0380
(Linear) (Six-base) MAPSORT of: KIEE0380.seq Check: 917 from: 1 to: 1743
KIEE0380.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 1172 1743
Size: 568 604 571
Fragments arranged by size:
604 571 568
AfeI AGC'GCT
Cuts at: 0 358 556 1743
Size: 358 198 1187
Fragments arranged by size:
1187 358 198
AflIII A'CryG_T
Cuts at: 0 1469 1743
Size: 1469 274
AhdI GACnn_n'nnGTC
Cuts at: 0 1417 1743
Size: 1417 326
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 352 774 1743
Size: 352 422 969
Fragments arranged by size:
969 422 352
AlwNI CAG_nnn'CTG
Cuts at: 0 871 1743
Size: 871 872
ApoI r'AATT_y
Cuts at: 0 1021 1168 1743
Size: 1021 147 575
Fragments arranged by size:
1021 575 147
AvaI C'yCGr_G
Cuts at: 0 172 352 1743
Size: 172 180 1391
Fragments arranged by size:
1391 180 172
BamHI G'GATC_C
Cuts at: 0 731 1743
Size: 731 1012
BanI G'GyrC_C
Cuts at: 0 207 1743
Size: 207 1536
BanII G_rGCy'C
Cuts at: 0 103 633 821 1181 1743
Size: 103 530 188 360 562
Fragments arranged by size:
562 530 360 188 103
BbvCI CC'TCA_GC
Cuts at: 0 319 1743
Size: 319 1424
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1552 1586 1743
Size: 1552 34 157
Fragments arranged by size:
1552 157 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 878 1307 1533 1743
Size: 878 429 226 210
Fragments arranged by size:
878 429 226 210
BfrBI ATG'CAT
Cuts at: 0 1554 1743
Size: 1554 189
Bme1580I G_kGCm'C
Cuts at: 0 763 1206 1743
Size: 763 443 537
Fragments arranged by size:
763 537 443
BmgBI CAC'GTC
Cuts at: 0 1472 1743
Size: 1472 271
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1033 1743
Size: 1033 710
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 733 861 1243 1330 1743
Size: 733 128 382 87 413
Fragments arranged by size:
733 413 382 128 87
Bpu10I CC'TnA_GC
Cuts at: 0 319 1743
Size: 319 1424
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 499 1743
Size: 499 1244
BsaI GGTCTCn'nnnn_
Cuts at: 0 923 1743
Size: 923 820
BsaAI yAC'GTr
Cuts at: 0 857 1743
Size: 857 886
BsaBI GATnn'nnATC
Cuts at: 0 310 1743
Size: 310 1433
BsaHI Gr'CG_yC
Cuts at: 0 646 1743
Size: 646 1097
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 1279 1309 1743
Size: 46 30 1203 30 434
Fragments arranged by size:
1203 434 46 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 280 298 757 1022 1743
Size: 280 18 459 265 721
Fragments arranged by size:
721 459 280 265 18
BseYI C'CCAG_C
Cuts at: 0 96 222 639 1743
Size: 96 126 417 1104
Fragments arranged by size:
1104 417 126 96
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 283 322 1166 1680 1743
Size: 283 39 844 514 63
Fragments arranged by size:
844 514 283 63 39
BsiEI CG_ry'CG
Cuts at: 0 51 202 327 1743
Size: 51 151 125 1416
Fragments arranged by size:
1416 151 125 51
BsiHKAI G_wGCw'C
Cuts at: 0 71 545 1181 1743
Size: 71 474 636 562
Fragments arranged by size:
636 562 474 71
Bsp1286I G_dGCh'C
Cuts at: 0 71 103 545 633 763 821 1181 1206
Size: 71 32 442 88 130 58 360 25
Cuts at: 1206 1743
Size: 537
Fragments arranged by size:
537 442 360 130 88 71 58 32
25
BsrDI GCAATG_nn'
Cuts at: 0 1517 1743
Size: 1517 226
BsrFI r'CCGG_y
Cuts at: 0 186 204 210 1743
Size: 186 18 6 1533
Fragments arranged by size:
1533 186 18 6
BssHII G'CGCG_C
Cuts at: 0 227 1743
Size: 227 1516
BstBI TT'CG_AA
Cuts at: 0 794 1743
Size: 794 949
BstYI r'GATC_y
Cuts at: 0 731 1034 1609 1743
Size: 731 303 575 134
Fragments arranged by size:
731 575 303 134
Bsu36I CC'TnA_GG
Cuts at: 0 1316 1508 1743
Size: 1316 192 235
Fragments arranged by size:
1316 235 192
BtgI C'CryG_G
Cuts at: 0 31 196 1743
Size: 31 165 1547
Fragments arranged by size:
1547 165 31
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 677 1174 1743
Size: 677 497 569
Fragments arranged by size:
677 569 497
BtsI GCAGTG_nn'
Cuts at: 0 334 758 1048 1743
Size: 334 424 290 695
Fragments arranged by size:
695 424 334 290
DraI TTT'AAA
Cuts at: 0 1173 1743
Size: 1173 570
EaeI y'GGCC_r
Cuts at: 0 48 89 199 560 1083 1130 1743
Size: 48 41 110 361 523 47 613
Fragments arranged by size:
613 523 361 110 48 47 41
EagI C'GGCC_G
Cuts at: 0 48 199 1743
Size: 48 151 1544
Fragments arranged by size:
1544 151 48
EarI CTCTTCn'nnn_
Cuts at: 0 734 1743
Size: 734 1009
EcoICRI GAG'CTC
Cuts at: 0 1179 1743
Size: 1179 564
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 733 861 1172 1243 1330 1743
Size: 568 165 128 311 71 87 413
Fragments arranged by size:
568 413 311 165 128 87 71
EcoO109I rG'GnC_Cy
Cuts at: 0 151 618 1743
Size: 151 467 1125
Fragments arranged by size:
1125 467 151
EcoRV GAT'ATC
Cuts at: 0 495 1743
Size: 495 1248
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 532 573 1299 1611 1743
Size: 532 41 726 312 132
Fragments arranged by size:
726 532 312 132 41
HaeII r_GCGC'y
Cuts at: 0 27 61 79 360 558 1743
Size: 27 34 18 281 198 1185
Fragments arranged by size:
1185 281 198 34 27 18
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 316 348 1281 1313 1403 1435 1456 1488
Size: 316 32 933 32 90 32 21 32
Cuts at: 1488 1594 1626 1743
Size: 106 32 117
Fragments arranged by size:
933 316 117 106 90 32 32 32
32 32 21
HincII GTy'rAC
Cuts at: 0 449 1743
Size: 449 1294
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 946 1743
Size: 946 797
MscI TGG'CCA
Cuts at: 0 562 1085 1132 1743
Size: 562 523 47 611
Fragments arranged by size:
611 562 523 47
MslI CAynn'nnrTG
Cuts at: 0 1068 1522 1743
Size: 1068 454 221
Fragments arranged by size:
1068 454 221
MspA1I CmG'CkG
Cuts at: 0 33 198 255 369 720 1150 1743
Size: 33 165 57 114 351 430 593
Fragments arranged by size:
593 430 351 165 114 57 33
NaeI GCC'GGC
Cuts at: 0 212 1743
Size: 212 1531
NgoMIV G'CCGG_C
Cuts at: 0 210 1743
Size: 210 1533
NotI GC'GGCC_GC
Cuts at: 0 199 1743
Size: 199 1544
NsiI A_TGCA'T
Cuts at: 0 1556 1743
Size: 1556 187
NspI r_CATG'y
Cuts at: 0 1312 1740 1743
Size: 1312 428 3
Fragments arranged by size:
1312 428 3
PflMI CCAn_nnn'nTGG
Cuts at: 0 1407 1743
Size: 1407 336
PfoI T'CCnGG_A
Cuts at: 0 677 1247 1743
Size: 677 570 496
Fragments arranged by size:
677 570 496
PmlI CAC'GTG
Cuts at: 0 857 1743
Size: 857 886
PpuMI rG'GwC_Cy
Cuts at: 0 151 1743
Size: 151 1592
PsiI TTA'TAA
Cuts at: 0 434 1743
Size: 434 1309
PstI C_TGCA'G
Cuts at: 0 254 260 281 728 1743
Size: 254 6 21 447 1015
Fragments arranged by size:
1015 447 254 21 6
PvuII CAG'CTG
Cuts at: 0 255 1150 1743
Size: 255 895 593
Fragments arranged by size:
895 593 255
SacI G_AGCT'C
Cuts at: 0 1181 1743
Size: 1181 562
SacII CC_GC'GG
Cuts at: 0 34 199 1743
Size: 34 165 1544
Fragments arranged by size:
1544 165 34
SanDI GG'GwC_CC
Cuts at: 0 151 1743
Size: 151 1592
SexAI A'CCwGG_T
Cuts at: 0 1415 1743
Size: 1415 328
SfcI C'TryA_G
Cuts at: 0 250 256 277 528 724 1743
Size: 250 6 21 251 196 1019
Fragments arranged by size:
1019 251 250 196 21 6
SgrAI Cr'CCGG_yG
Cuts at: 0 204 1743
Size: 204 1539
SmaI CCC'GGG
Cuts at: 0 174 1743
Size: 174 1569
SmlI C'TyrA_G
Cuts at: 0 478 1743
Size: 478 1265
SspI AAT'ATT
Cuts at: 0 961 1743
Size: 961 782
StuI AGG'CCT
Cuts at: 0 1326 1743
Size: 1326 417
StyI C'CwwG_G
Cuts at: 0 584 1743
Size: 584 1159
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1082 1513 1743
Size: 1082 431 230
Fragments arranged by size:
1082 431 230
TatI w'GTAC_w
Cuts at: 0 1466 1743
Size: 1466 277
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1057 1089 1612 1644 1743
Size: 1057 32 523 32 99
Fragments arranged by size:
1057 523 99 32 32
XmaI C'CCGG_G
Cuts at: 0 172 1743
Size: 172 1571
XmnI GAAnn'nnTTC
Cuts at: 0 790 1720 1743
Size: 790 930 23
Fragments arranged by size:
930 790 23
Enzymes that do cut:
AcuI AfeI AflIII AhdI AlfI AlwNI ApoI AvaI
BamHI BanI BanII BbvCI BcgI BciVI BfrBI Bme1580I
BmgBI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BsrDI BsrFI BssHII BstBI BstYI Bsu36I BtgI BtgZI
BtsI DraI EaeI EagI EarI EcoICRI Eco57MI EcoO109I
EcoRV FalI HaeII Hin4I HincII MmeI MscI MslI
MspA1I NaeI NgoMIV NotI NsiI NspI PflMI PfoI
PmlI PpuMI PsiI PstI PvuII SacI SacII SanDI
SexAI SfcI SgrAI SmaI SmlI SspI StuI StyI
TaqII TatI TstI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AflII AgeI AleI
AloI ApaI ApaLI AscI AseI AsiSI AvrII BaeI
BbeI BbsI BclI BglI BglII BlpI BmrI BmtI
BsaWI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI
BsrGI BssSI BstAPI BstEII BstXI BstZ17I ClaI CspCI
DraIII DrdI EciI EcoNI EcoRI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NruI PacI PasI PciI PmeI PpiI
PshAI PspOMI PspXI PsrI PvuI RsrII SalI SapI
SbfI ScaI SfiI SfoI SgfI SnaBI SpeI SphI
SrfI SwaI Tth111I XbaI XcmI XhoI ZraI