Restriction map for
pF1KE0372
(Linear) (Six-base) MAPSORT of: KIEE0372.seq Check: 9058 from: 1 to: 1695
KIEE0372.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AatII G_ACGT'C
Cuts at: 0 1391 1695
Size: 1391 304
AccI GT'mk_AC
Cuts at: 0 648 1181 1215 1695
Size: 648 533 34 480
Fragments arranged by size:
648 533 480 34
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 1187 1695
Size: 89 1098 508
Fragments arranged by size:
1098 508 89
AflII C'TTAA_G
Cuts at: 0 1392 1695
Size: 1392 303
AflIII A'CryG_T
Cuts at: 0 472 1633 1695
Size: 472 1161 62
Fragments arranged by size:
1161 472 62
AlwNI CAG_nnn'CTG
Cuts at: 0 127 1695
Size: 127 1568
ApoI r'AATT_y
Cuts at: 0 400 756 1189 1695
Size: 400 356 433 506
Fragments arranged by size:
506 433 400 356
AseI AT'TA_AT
Cuts at: 0 850 1695
Size: 850 845
BanII G_rGCy'C
Cuts at: 0 79 469 1695
Size: 79 390 1226
Fragments arranged by size:
1226 390 79
BbsI GAAGACnn'nnnn_
Cuts at: 0 3 797 1695
Size: 3 794 898
Fragments arranged by size:
898 794 3
BclI T'GATC_A
Cuts at: 0 513 854 1695
Size: 513 341 841
Fragments arranged by size:
841 513 341
BglII A'GATC_T
Cuts at: 0 552 1695
Size: 552 1143
Bme1580I G_kGCm'C
Cuts at: 0 300 1695
Size: 300 1395
BmgBI CAC'GTC
Cuts at: 0 1594 1695
Size: 1594 101
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 173 1157 1695
Size: 173 984 538
Fragments arranged by size:
984 538 173
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 398 977 1130 1695
Size: 398 579 153 565
Fragments arranged by size:
579 565 398 153
Bpu10I CC'TnA_GC
Cuts at: 0 1132 1695
Size: 1132 563
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 655 1695
Size: 655 1040
BsaI GGTCTCn'nnnn_
Cuts at: 0 375 1695
Size: 375 1320
BsaAI yAC'GTr
Cuts at: 0 475 1695
Size: 475 1220
BsaHI Gr'CG_yC
Cuts at: 0 1388 1695
Size: 1388 307
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1106 1136 1220 1250 1695
Size: 1106 30 84 30 445
Fragments arranged by size:
1106 445 84 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 505 680 1133 1695
Size: 505 175 453 562
Fragments arranged by size:
562 505 453 175
BsiHKAI G_wGCw'C
Cuts at: 0 79 1695
Size: 79 1616
BsmI GAATG_Cn'
Cuts at: 0 1177 1695
Size: 1177 518
Bsp1286I G_dGCh'C
Cuts at: 0 79 300 469 1695
Size: 79 221 169 1226
Fragments arranged by size:
1226 221 169 79
BsrDI GCAATG_nn'
Cuts at: 0 98 1082 1695
Size: 98 984 613
Fragments arranged by size:
984 613 98
BstAPI GCAn_nnn'nTGC
Cuts at: 0 889 1695
Size: 889 806
BstYI r'GATC_y
Cuts at: 0 552 1695
Size: 552 1143
BstZ17I GTA'TAC
Cuts at: 0 1216 1695
Size: 1216 479
Bsu36I CC'TnA_GG
Cuts at: 0 1110 1695
Size: 1110 585
BtsI GCAGTG_nn'
Cuts at: 0 1294 1695
Size: 1294 401
DrdI GACnn_nn'nnGTC
Cuts at: 0 1387 1695
Size: 1387 308
EaeI y'GGCC_r
Cuts at: 0 744 1275 1524 1695
Size: 744 531 249 171
Fragments arranged by size:
744 531 249 171
EarI CTCTTCn'nnn_
Cuts at: 0 974 1695
Size: 974 721
EcoICRI GAG'CTC
Cuts at: 0 77 1695
Size: 77 1618
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 89 398 977 1130 1187 1695
Size: 89 309 579 153 57 508
Fragments arranged by size:
579 508 309 153 89 57
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 378 917 1695
Size: 378 539 778
Fragments arranged by size:
778 539 378
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 862 894 1583 1615 1695
Size: 862 32 689 32 80
Fragments arranged by size:
862 689 80 32 32
HincII GTy'rAC
Cuts at: 0 1641 1695
Size: 1641 54
HindIII A'AGCT_T
Cuts at: 0 1049 1695
Size: 1049 646
HpaI GTT'AAC
Cuts at: 0 1641 1695
Size: 1641 54
MfeI C'AATT_G
Cuts at: 0 287 1295 1695
Size: 287 1008 400
Fragments arranged by size:
1008 400 287
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 288 1542 1695
Size: 288 1254 153
Fragments arranged by size:
1254 288 153
MscI TGG'CCA
Cuts at: 0 746 1277 1695
Size: 746 531 418
Fragments arranged by size:
746 531 418
MslI CAynn'nnrTG
Cuts at: 0 364 451 947 988 1438 1695
Size: 364 87 496 41 450 257
Fragments arranged by size:
496 450 364 257 87 41
MspA1I CmG'CkG
Cuts at: 0 376 1695
Size: 376 1319
NspI r_CATG'y
Cuts at: 0 1637 1695
Size: 1637 58
PciI A'CATG_T
Cuts at: 0 1633 1695
Size: 1633 62
PflMI CCAn_nnn'nTGG
Cuts at: 0 1500 1695
Size: 1500 195
PfoI T'CCnGG_A
Cuts at: 0 172 1695
Size: 172 1523
PsiI TTA'TAA
Cuts at: 0 1057 1208 1695
Size: 1057 151 487
Fragments arranged by size:
1057 487 151
PstI C_TGCA'G
Cuts at: 0 885 1695
Size: 885 810
PvuII CAG'CTG
Cuts at: 0 376 1695
Size: 376 1319
SacI G_AGCT'C
Cuts at: 0 79 1695
Size: 79 1616
SfcI C'TryA_G
Cuts at: 0 645 881 1695
Size: 645 236 814
Fragments arranged by size:
814 645 236
SmlI C'TyrA_G
Cuts at: 0 634 1392 1695
Size: 634 758 303
Fragments arranged by size:
758 634 303
SspI AAT'ATT
Cuts at: 0 675 979 1695
Size: 675 304 716
Fragments arranged by size:
716 675 304
StyI C'CwwG_G
Cuts at: 0 68 160 1695
Size: 68 92 1535
Fragments arranged by size:
1535 92 68
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 585 1695
Size: 585 1110
Tth111I GACn'n_nGTC
Cuts at: 0 1303 1695
Size: 1303 392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 180 271 1497 1695
Size: 180 91 1226 198
Fragments arranged by size:
1226 198 180 91
ZraI GAC'GTC
Cuts at: 0 1389 1695
Size: 1389 306
Enzymes that do cut:
AatII AccI AcuI AflII AflIII AlwNI ApoI AseI
BanII BbsI BclI BglII Bme1580I BmgBI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BsiHKAI
BsmI Bsp1286I BsrDI BstAPI BstYI BstZ17I Bsu36I BtsI
DrdI EaeI EarI EcoICRI Eco57MI FalI Hin4I HincII
HindIII HpaI MfeI MmeI MscI MslI MspA1I NspI
PciI PflMI PfoI PsiI PstI PvuII SacI SfcI
SmlI SspI StyI TaqII Tth111I XcmI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AfeI AgeI AhdI AleI AlfI
AloI ApaI ApaLI AscI AsiSI AvaI AvrII BaeI
BamHI BanI BbeI BbvCI BcgI BciVI BfrBI BglI
BlpI BmrI BmtI BsaBI BsaWI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BspMI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstEII BstXI BtgI BtgZI ClaI
CspCI DraI DraIII EagI EciI EcoNI EcoO109I EcoRI
EcoRV FseI FspI FspAI HaeII KasI KpnI MluI
NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PmeI PmlI PpiI PpuMI PshAI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SanDI
SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI StuI SwaI TatI
TstI XbaI XhoI XmaI XmnI