Restriction map for
pF1KE0371
(Linear) (Six-base) MAPSORT of: KIEE0371.seq Check: 9245 from: 1 to: 1686
KIEE0371.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 1673 1686
Size: 1673 13
Acc65I G'GTAC_C
Cuts at: 0 270 1686
Size: 270 1416
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1686
Size: 1397 289
AflIII A'CryG_T
Cuts at: 0 182 1253 1364 1686
Size: 182 1071 111 322
Fragments arranged by size:
1071 322 182 111
AleI CACnn'nnGTG
Cuts at: 0 181 1686
Size: 181 1505
AlwNI CAG_nnn'CTG
Cuts at: 0 972 1191 1686
Size: 972 219 495
Fragments arranged by size:
972 495 219
ApaI G_GGCC'C
Cuts at: 0 25 58 1545 1686
Size: 25 33 1487 141
Fragments arranged by size:
1487 141 33 25
ApaLI G'TGCA_C
Cuts at: 0 577 1686
Size: 577 1109
AvaI C'yCGr_G
Cuts at: 0 1288 1390 1686
Size: 1288 102 296
Fragments arranged by size:
1288 296 102
BamHI G'GATC_C
Cuts at: 0 227 1686
Size: 227 1459
BanI G'GyrC_C
Cuts at: 0 42 270 1120 1686
Size: 42 228 850 566
Fragments arranged by size:
850 566 228 42
BanII G_rGCy'C
Cuts at: 0 25 37 58 1205 1325 1545 1686
Size: 25 12 21 1147 120 220 141
Fragments arranged by size:
1147 220 141 120 25 21 12
BbeI G_GCGC'C
Cuts at: 0 46 1686
Size: 46 1640
BbsI GAAGACnn'nnnn_
Cuts at: 0 348 909 1686
Size: 348 561 777
Fragments arranged by size:
777 561 348
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 192 226 1686
Size: 192 34 1460
Fragments arranged by size:
1460 192 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1034 1540 1686
Size: 1034 506 146
Fragments arranged by size:
1034 506 146
BglII A'GATC_T
Cuts at: 0 701 1686
Size: 701 985
BlpI GC'TnA_GC
Cuts at: 0 1295 1686
Size: 1295 391
Bme1580I G_kGCm'C
Cuts at: 0 25 58 581 1180 1545 1686
Size: 25 33 523 599 365 141
Fragments arranged by size:
599 523 365 141 33 25
BmgBI CAC'GTC
Cuts at: 0 183 489 1104 1686
Size: 183 306 615 582
Fragments arranged by size:
615 582 306 183
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1686
Size: 360 1326
Bpu10I CC'TnA_GC
Cuts at: 0 1030 1686
Size: 1030 656
BsaI GGTCTCn'nnnn_
Cuts at: 0 471 520 853 1686
Size: 471 49 333 833
Fragments arranged by size:
833 471 333 49
BsaAI yAC'GTr
Cuts at: 0 1677 1686
Size: 1677 9
BsaHI Gr'CG_yC
Cuts at: 0 43 1467 1686
Size: 43 1424 219
Fragments arranged by size:
1424 219 43
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1573 1603 1686
Size: 1573 30 83
Fragments arranged by size:
1573 83 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 112 232 680 1686
Size: 22 90 120 448 1006
Fragments arranged by size:
1006 448 120 90 22
BseYI C'CCAG_C
Cuts at: 0 1235 1405 1686
Size: 1235 170 281
Fragments arranged by size:
1235 281 170
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 114 321 397 1686
Size: 114 207 76 1289
Fragments arranged by size:
1289 207 114 76
BsiEI CG_ry'CG
Cuts at: 0 560 662 692 1686
Size: 560 102 30 994
Fragments arranged by size:
994 560 102 30
BsiHKAI G_wGCw'C
Cuts at: 0 581 1686
Size: 581 1105
BsmBI CGTCTCn'nnnn_
Cuts at: 0 634 1446 1458 1686
Size: 634 812 12 228
Fragments arranged by size:
812 634 228 12
Bsp1286I G_dGCh'C
Cuts at: 0 25 37 58 581 1180 1205 1325 1545
Size: 25 12 21 523 599 25 120 220
Cuts at: 1545 1686
Size: 141
Fragments arranged by size:
599 523 220 141 120 25 25 21
12
BspHI T'CATG_A
Cuts at: 0 1226 1686
Size: 1226 460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1199 1686
Size: 1199 487
BsrBI CCG'CTC
Cuts at: 0 944 1034 1686
Size: 944 90 652
Fragments arranged by size:
944 652 90
BsrDI GCAATG_nn'
Cuts at: 0 1515 1686
Size: 1515 171
BsrFI r'CCGG_y
Cuts at: 0 364 1686
Size: 364 1322
BstXI CCAn_nnnn'nTGG
Cuts at: 0 540 552 1665 1686
Size: 540 12 1113 21
Fragments arranged by size:
1113 540 21 12
BstYI r'GATC_y
Cuts at: 0 227 407 498 701 1686
Size: 227 180 91 203 985
Fragments arranged by size:
985 227 203 180 91
BtgI C'CryG_G
Cuts at: 0 560 1067 1686
Size: 560 507 619
Fragments arranged by size:
619 560 507
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 217 1686
Size: 217 1469
BtsI GCAGTG_nn'
Cuts at: 0 197 1219 1436 1686
Size: 197 1022 217 250
Fragments arranged by size:
1022 250 217 197
DraIII CAC_nnn'GTG
Cuts at: 0 1653 1686
Size: 1653 33
DrdI GACnn_nn'nnGTC
Cuts at: 0 658 1686
Size: 658 1028
EaeI y'GGCC_r
Cuts at: 0 81 164 542 557 689 819 1433 1686
Size: 81 83 378 15 132 130 614 253
Fragments arranged by size:
614 378 253 132 130 83 81 15
EagI C'GGCC_G
Cuts at: 0 557 689 1686
Size: 557 132 997
Fragments arranged by size:
997 557 132
EarI CTCTTCn'nnn_
Cuts at: 0 238 760 856 901 1686
Size: 238 522 96 45 785
Fragments arranged by size:
785 522 238 96 45
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 161 1362 1686
Size: 161 1201 324
Fragments arranged by size:
1201 324 161
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 360 1397 1686
Size: 360 1037 289
Fragments arranged by size:
1037 360 289
EcoNI CCTnn'n_nnAGG
Cuts at: 0 424 1666 1686
Size: 424 1242 20
Fragments arranged by size:
1242 424 20
EcoO109I rG'GnC_Cy
Cuts at: 0 21 22 55 969 1188 1541 1686
Size: 21 1 33 914 219 353 145
Fragments arranged by size:
914 353 219 145 33 21 1
HaeII r_GCGC'y
Cuts at: 0 46 1686
Size: 46 1640
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 379 411 1686
Size: 379 32 1275
Fragments arranged by size:
1275 379 32
HincII GTy'rAC
Cuts at: 0 1611 1686
Size: 1611 75
KasI G'GCGC_C
Cuts at: 0 42 1686
Size: 42 1644
KpnI G_GTAC'C
Cuts at: 0 274 1686
Size: 274 1412
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 642 1686
Size: 642 1044
MscI TGG'CCA
Cuts at: 0 544 821 1435 1686
Size: 544 277 614 251
Fragments arranged by size:
614 544 277 251
MslI CAynn'nnrTG
Cuts at: 0 181 202 550 1686
Size: 181 21 348 1136
Fragments arranged by size:
1136 348 181 21
MspA1I CmG'CkG
Cuts at: 0 143 1422 1686
Size: 143 1279 264
Fragments arranged by size:
1279 264 143
NarI GG'CG_CC
Cuts at: 0 43 1686
Size: 43 1643
NcoI C'CATG_G
Cuts at: 0 1067 1686
Size: 1067 619
NspI r_CATG'y
Cuts at: 0 649 1257 1647 1686
Size: 649 608 390 39
Fragments arranged by size:
649 608 390 39
PasI CC'CwG_GG
Cuts at: 0 18 59 1128 1686
Size: 18 41 1069 558
Fragments arranged by size:
1069 558 41 18
PciI A'CATG_T
Cuts at: 0 1253 1686
Size: 1253 433
PfoI T'CCnGG_A
Cuts at: 0 854 1686
Size: 854 832
PpuMI rG'GwC_Cy
Cuts at: 0 1188 1686
Size: 1188 498
PspOMI G'GGCC_C
Cuts at: 0 21 54 1541 1686
Size: 21 33 1487 145
Fragments arranged by size:
1487 145 33 21
PstI C_TGCA'G
Cuts at: 0 685 1686
Size: 685 1001
SapI GCTCTTCn'nnn_
Cuts at: 0 901 1686
Size: 901 785
SexAI A'CCwGG_T
Cuts at: 0 505 1686
Size: 505 1181
SfcI C'TryA_G
Cuts at: 0 594 681 984 1686
Size: 594 87 303 702
Fragments arranged by size:
702 594 303 87
SfoI GGC'GCC
Cuts at: 0 44 1686
Size: 44 1642
SmaI CCC'GGG
Cuts at: 0 1290 1392 1686
Size: 1290 102 294
Fragments arranged by size:
1290 294 102
SphI G_CATG'C
Cuts at: 0 649 1647 1686
Size: 649 998 39
Fragments arranged by size:
998 649 39
StyI C'CwwG_G
Cuts at: 0 26 1019 1067 1316 1686
Size: 26 993 48 249 370
Fragments arranged by size:
993 370 249 48 26
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1108 1228 1398 1686
Size: 1108 120 170 288
Fragments arranged by size:
1108 288 170 120
TatI w'GTAC_w
Cuts at: 0 746 1686
Size: 746 940
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 63 95 1354 1386 1639 1671 1686
Size: 63 32 1259 32 253 32 15
Fragments arranged by size:
1259 253 63 32 32 32 15
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 969 1095 1164 1392 1686
Size: 969 126 69 228 294
Fragments arranged by size:
969 294 228 126 69
XmaI C'CCGG_G
Cuts at: 0 1288 1390 1686
Size: 1288 102 296
Fragments arranged by size:
1288 296 102
Enzymes that do cut:
AccI Acc65I AcuI AflIII AleI AlwNI ApaI ApaLI
AvaI BamHI BanI BanII BbeI BbsI BcgI BglI
BglII BlpI Bme1580I BmgBI BpmI Bpu10I BsaI BsaAI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI
Bsp1286I BspHI BspMI BsrBI BsrDI BsrFI BstXI BstYI
BtgI BtgZI BtsI DraIII DrdI EaeI EagI EarI
EciI Eco57MI EcoNI EcoO109I HaeII Hin4I HincII KasI
KpnI MmeI MscI MslI MspA1I NarI NcoI NspI
PasI PciI PfoI PpuMI PspOMI PstI SapI SexAI
SfcI SfoI SmaI SphI StyI TaqII TatI TstI
XcmI XmaI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AlfI
AloI ApoI AscI AseI AsiSI AvrII BaeI BbvCI
BciVI BclI BfrBI BmrI BmtI BplI BpuEI BsaBI
BsaWI BsiWI BsmI BspEI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstZ17I Bsu36I ClaI CspCI DraI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HindIII HpaI
MfeI MluI NaeI NdeI NgoMIV NheI NotI NruI
NsiI PacI PflMI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SbfI ScaI SfiI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmnI ZraI