Restriction map for
pF1KE0353
(Linear) (Six-base) MAPSORT of: KIEE0353.seq Check: 7269 from: 1 to: 1629
KIEE0353.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AccI GT'mk_AC
Cuts at: 0 938 1629
Size: 938 691
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1629
Size: 1397 232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 174 668 1629
Size: 174 494 961
Fragments arranged by size:
961 494 174
AlwNI CAG_nnn'CTG
Cuts at: 0 230 1044 1420 1629
Size: 230 814 376 209
Fragments arranged by size:
814 376 230 209
ApoI r'AATT_y
Cuts at: 0 1618 1629
Size: 1618 11
BamHI G'GATC_C
Cuts at: 0 428 1629
Size: 428 1201
BanII G_rGCy'C
Cuts at: 0 348 1629
Size: 348 1281
BbsI GAAGACnn'nnnn_
Cuts at: 0 248 628 1629
Size: 248 380 1001
Fragments arranged by size:
1001 380 248
BbvCI CC'TCA_GC
Cuts at: 0 122 1629
Size: 122 1507
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 856 890 1629
Size: 856 34 739
Fragments arranged by size:
856 739 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 615 1629
Size: 615 1014
BclI T'GATC_A
Cuts at: 0 221 1629
Size: 221 1408
BfrBI ATG'CAT
Cuts at: 0 205 1629
Size: 205 1424
BglII A'GATC_T
Cuts at: 0 363 490 1200 1264 1629
Size: 363 127 710 64 365
Fragments arranged by size:
710 365 363 127 64
Bme1580I G_kGCm'C
Cuts at: 0 1427 1629
Size: 1427 202
BmrI ACTGGGnnnn_n'
Cuts at: 0 1395 1430 1629
Size: 1395 35 199
Fragments arranged by size:
1395 199 35
Bpu10I CC'TnA_GC
Cuts at: 0 122 298 1629
Size: 122 176 1331
Fragments arranged by size:
1331 176 122
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 558 588 753 783 1104 1134 1629
Size: 558 30 165 30 321 30 495
Fragments arranged by size:
558 495 321 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 391 420 430 1629
Size: 391 29 10 1199
Fragments arranged by size:
1199 391 29 10
BseYI C'CCAG_C
Cuts at: 0 800 1629
Size: 800 829
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1312 1629
Size: 1312 317
BsmI GAATG_Cn'
Cuts at: 0 104 205 1629
Size: 104 101 1424
Fragments arranged by size:
1424 104 101
Bsp1286I G_dGCh'C
Cuts at: 0 348 1427 1629
Size: 348 1079 202
Fragments arranged by size:
1079 348 202
BspHI T'CATG_A
Cuts at: 0 512 1629
Size: 512 1117
BsrDI GCAATG_nn'
Cuts at: 0 650 823 1629
Size: 650 173 806
Fragments arranged by size:
806 650 173
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 1629
Size: 591 1038
BstYI r'GATC_y
Cuts at: 0 363 428 490 1200 1264 1629
Size: 363 65 62 710 64 365
Fragments arranged by size:
710 365 363 65 64 62
Bsu36I CC'TnA_GG
Cuts at: 0 1182 1629
Size: 1182 447
BtsI GCAGTG_nn'
Cuts at: 0 747 811 1032 1212 1629
Size: 747 64 221 180 417
Fragments arranged by size:
747 417 221 180 64
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 41 76 1629
Size: 41 35 1553
Fragments arranged by size:
1553 41 35
EarI CTCTTCn'nnn_
Cuts at: 0 91 418 825 1629
Size: 91 327 407 804
Fragments arranged by size:
804 407 327 91
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1397 1629
Size: 1397 232
EcoNI CCTnn'n_nnAGG
Cuts at: 0 446 1629
Size: 446 1183
EcoO109I rG'GnC_Cy
Cuts at: 0 302 766 1170 1629
Size: 302 464 404 459
Fragments arranged by size:
464 459 404 302
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1308 1629
Size: 1308 321
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 130 162 342 374 1051 1083 1629
Size: 130 32 180 32 677 32 546
Fragments arranged by size:
677 546 180 130 32 32 32
HincII GTy'rAC
Cuts at: 0 1548 1629
Size: 1548 81
MslI CAynn'nnrTG
Cuts at: 0 250 1629
Size: 250 1379
NsiI A_TGCA'T
Cuts at: 0 207 1629
Size: 207 1422
PasI CC'CwG_GG
Cuts at: 0 1016 1629
Size: 1016 613
PpuMI rG'GwC_Cy
Cuts at: 0 1170 1629
Size: 1170 459
PsiI TTA'TAA
Cuts at: 0 728 1629
Size: 728 901
PstI C_TGCA'G
Cuts at: 0 1082 1219 1629
Size: 1082 137 410
Fragments arranged by size:
1082 410 137
SapI GCTCTTCn'nnn_
Cuts at: 0 825 1629
Size: 825 804
SfcI C'TryA_G
Cuts at: 0 1078 1215 1629
Size: 1078 137 414
Fragments arranged by size:
1078 414 137
SspI AAT'ATT
Cuts at: 0 689 790 1629
Size: 689 101 839
Fragments arranged by size:
839 689 101
StyI C'CwwG_G
Cuts at: 0 922 1629
Size: 922 707
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 10 1629
Size: 10 1619
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 558 590 1629
Size: 558 32 1039
Fragments arranged by size:
1039 558 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 495 1069 1629
Size: 495 574 560
Fragments arranged by size:
574 560 495
XmnI GAAnn'nnTTC
Cuts at: 0 689 1629
Size: 689 940
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApoI BamHI BanII BbsI
BbvCI BcgI BciVI BclI BfrBI BglII Bme1580I BmrI
Bpu10I BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspHI
BsrDI BstXI BstYI Bsu36I BtsI CspCI EarI Eco57MI
EcoNI EcoO109I FalI Hin4I HincII MslI NsiI PasI
PpuMI PsiI PstI SapI SfcI SspI StyI TaqII
TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BanI BbeI BglI BlpI BmgBI
BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI
BsaWI BsiEI BsiHKAI BsiWI BsmBI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstZ17I
BtgI BtgZI ClaI DraI DraIII DrdI EaeI EagI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HaeII
HindIII HpaI KasI KpnI MfeI MluI MmeI MscI
MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PciI PflMI PfoI PmeI PmlI
PpiI PshAI PspOMI PspXI PsrI PvuI PvuII RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TatI Tth111I XbaI XhoI XmaI
ZraI