Restriction map for
pF1KE0345
(Linear) (Six-base) MAPSORT of: KIEE0345.seq Check: 9457 from: 1 to: 1707
KIEE0345.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1323 1707
Size: 1323 384
Acc65I G'GTAC_C
Cuts at: 0 1302 1707
Size: 1302 405
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1254 1707
Size: 1254 453
AleI CACnn'nnGTG
Cuts at: 0 1540 1707
Size: 1540 167
AlwNI CAG_nnn'CTG
Cuts at: 0 1524 1570 1707
Size: 1524 46 137
Fragments arranged by size:
1524 137 46
ApaI G_GGCC'C
Cuts at: 0 851 1068 1707
Size: 851 217 639
Fragments arranged by size:
851 639 217
AvaI C'yCGr_G
Cuts at: 0 896 916 1111 1707
Size: 896 20 195 596
Fragments arranged by size:
896 596 195 20
BamHI G'GATC_C
Cuts at: 0 1241 1707
Size: 1241 466
BanI G'GyrC_C
Cuts at: 0 399 670 1302 1589 1707
Size: 399 271 632 287 118
Fragments arranged by size:
632 399 287 271 118
BanII G_rGCy'C
Cuts at: 0 230 462 851 916 1068 1505 1707
Size: 230 232 389 65 152 437 202
Fragments arranged by size:
437 389 232 230 202 152 65
BbvCI CC'TCA_GC
Cuts at: 0 81 302 749 1707
Size: 81 221 447 958
Fragments arranged by size:
958 447 221 81
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 234 268 1652 1686 1707
Size: 234 34 1384 34 21
Fragments arranged by size:
1384 234 34 34 21
BglI GCCn_nnn'nGGC
Cuts at: 0 1385 1707
Size: 1385 322
BglII A'GATC_T
Cuts at: 0 1070 1707
Size: 1070 637
BlpI GC'TnA_GC
Cuts at: 0 773 841 1707
Size: 773 68 866
Fragments arranged by size:
866 773 68
Bme1580I G_kGCm'C
Cuts at: 0 675 851 1068 1141 1363 1423 1707
Size: 675 176 217 73 222 60 284
Fragments arranged by size:
675 284 222 217 176 73 60
BmgBI CAC'GTC
Cuts at: 0 813 1482 1707
Size: 813 669 225
Fragments arranged by size:
813 669 225
BmrI ACTGGGnnnn_n'
Cuts at: 0 824 1707
Size: 824 883
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1600 1662 1707
Size: 1600 62 45
Fragments arranged by size:
1600 62 45
Bpu10I CC'TnA_GC
Cuts at: 0 81 302 749 1707
Size: 81 221 447 958
Fragments arranged by size:
958 447 221 81
BsaI GGTCTCn'nnnn_
Cuts at: 0 1576 1707
Size: 1576 131
BsaWI w'CCGG_w
Cuts at: 0 959 1394 1707
Size: 959 435 313
Fragments arranged by size:
959 435 313
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 800 830 877 907 1021 1051 1081 1111
Size: 800 30 47 30 114 30 30 30
Cuts at: 1111 1198 1228 1707
Size: 87 30 479
Fragments arranged by size:
800 479 114 87 47 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1513 1707
Size: 1513 194
BseYI C'CCAG_C
Cuts at: 0 21 475 1055 1279 1414 1658 1707
Size: 21 454 580 224 135 244 49
Fragments arranged by size:
580 454 244 224 135 49 21
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1427 1495 1707
Size: 1427 68 212
Fragments arranged by size:
1427 212 68
BsiEI CG_ry'CG
Cuts at: 0 731 1707
Size: 731 976
BsiHKAI G_wGCw'C
Cuts at: 0 151 788 1707
Size: 151 637 919
Fragments arranged by size:
919 637 151
BsmI GAATG_Cn'
Cuts at: 0 240 1707
Size: 240 1467
BsmBI CGTCTCn'nnnn_
Cuts at: 0 646 803 1707
Size: 646 157 904
Fragments arranged by size:
904 646 157
Bsp1286I G_dGCh'C
Cuts at: 0 151 230 462 675 788 851 916 1068
Size: 151 79 232 213 113 63 65 152
Cuts at: 1068 1141 1363 1423 1505 1707
Size: 73 222 60 82 202
Fragments arranged by size:
232 222 213 202 152 151 113 82
79 73 65 63 60
BspEI T'CCGG_A
Cuts at: 0 1394 1707
Size: 1394 313
BspHI T'CATG_A
Cuts at: 0 230 1707
Size: 230 1477
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1323 1707
Size: 1323 384
BsrBI CCG'CTC
Cuts at: 0 840 1226 1347 1707
Size: 840 386 121 360
Fragments arranged by size:
840 386 360 121
BsrDI GCAATG_nn'
Cuts at: 0 203 1707
Size: 203 1504
BsrFI r'CCGG_y
Cuts at: 0 1699 1707
Size: 1699 8
BsrGI T'GTAC_A
Cuts at: 0 110 821 1707
Size: 110 711 886
Fragments arranged by size:
886 711 110
BstAPI GCAn_nnn'nTGC
Cuts at: 0 205 1570 1707
Size: 205 1365 137
Fragments arranged by size:
1365 205 137
BstXI CCAn_nnnn'nTGG
Cuts at: 0 442 1005 1446 1707
Size: 442 563 441 261
Fragments arranged by size:
563 442 441 261
BstYI r'GATC_y
Cuts at: 0 410 1070 1241 1707
Size: 410 660 171 466
Fragments arranged by size:
660 466 410 171
BtgI C'CryG_G
Cuts at: 0 35 125 131 506 1433 1493 1707
Size: 35 90 6 375 927 60 214
Fragments arranged by size:
927 375 214 90 60 35 6
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1605 1707
Size: 1605 102
BtsI GCAGTG_nn'
Cuts at: 0 151 169 1139 1179 1439 1571 1707
Size: 151 18 970 40 260 132 136
Fragments arranged by size:
970 260 151 136 132 40 18
DrdI GACnn_nn'nnGTC
Cuts at: 0 1390 1707
Size: 1390 317
EaeI y'GGCC_r
Cuts at: 0 32 128 659 728 1386 1436 1448 1707
Size: 32 96 531 69 658 50 12 259
Fragments arranged by size:
658 531 259 96 69 50 32 12
EagI C'GGCC_G
Cuts at: 0 728 1707
Size: 728 979
EarI CTCTTCn'nnn_
Cuts at: 0 271 472 1707
Size: 271 201 1235
Fragments arranged by size:
1235 271 201
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1173 1707
Size: 1173 534
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1254 1600 1662 1707
Size: 1254 346 62 45
Fragments arranged by size:
1254 346 62 45
EcoO109I rG'GnC_Cy
Cuts at: 0 753 927 980 993 1707
Size: 753 174 53 13 714
Fragments arranged by size:
753 714 174 53 13
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 169 201 1258 1290 1707
Size: 169 32 1057 32 417
Fragments arranged by size:
1057 417 169 32 32
KpnI G_GTAC'C
Cuts at: 0 1306 1707
Size: 1306 401
MscI TGG'CCA
Cuts at: 0 661 1450 1707
Size: 661 789 257
Fragments arranged by size:
789 661 257
MslI CAynn'nnrTG
Cuts at: 0 1540 1624 1707
Size: 1540 84 83
Fragments arranged by size:
1540 84 83
MspA1I CmG'CkG
Cuts at: 0 133 438 486 636 748 792 845 1238
Size: 133 305 48 150 112 44 53 393
Cuts at: 1238 1666 1707
Size: 428 41
Fragments arranged by size:
428 393 305 150 133 112 53 48
44 41
NaeI GCC'GGC
Cuts at: 0 1701 1707
Size: 1701 6
NgoMIV G'CCGG_C
Cuts at: 0 1699 1707
Size: 1699 8
NspI r_CATG'y
Cuts at: 0 828 1707
Size: 828 879
PasI CC'CwG_GG
Cuts at: 0 455 1707
Size: 455 1252
PflMI CCAn_nnn'nTGG
Cuts at: 0 246 1707
Size: 246 1461
PfoI T'CCnGG_A
Cuts at: 0 1678 1707
Size: 1678 29
PpuMI rG'GwC_Cy
Cuts at: 0 753 980 993 1707
Size: 753 227 13 714
Fragments arranged by size:
753 714 227 13
PspOMI G'GGCC_C
Cuts at: 0 847 1064 1707
Size: 847 217 643
Fragments arranged by size:
847 643 217
PstI C_TGCA'G
Cuts at: 0 1575 1707
Size: 1575 132
PvuII CAG'CTG
Cuts at: 0 486 636 748 1666 1707
Size: 486 150 112 918 41
Fragments arranged by size:
918 486 150 112 41
RsrII CG'GwC_CG
Cuts at: 0 545 1707
Size: 545 1162
SacII CC_GC'GG
Cuts at: 0 134 1707
Size: 134 1573
SanDI GG'GwC_CC
Cuts at: 0 993 1707
Size: 993 714
SfcI C'TryA_G
Cuts at: 0 1571 1707
Size: 1571 136
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1385 1707
Size: 1385 322
SmaI CCC'GGG
Cuts at: 0 898 1707
Size: 898 809
StuI AGG'CCT
Cuts at: 0 679 1707
Size: 679 1028
StyI C'CwwG_G
Cuts at: 0 89 1217 1707
Size: 89 1128 490
Fragments arranged by size:
1128 490 89
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1615 1707
Size: 1615 92
TatI w'GTAC_w
Cuts at: 0 110 821 1707
Size: 110 711 886
Fragments arranged by size:
886 711 110
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 800 832 1081 1113 1707
Size: 800 32 249 32 594
Fragments arranged by size:
800 594 249 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 123 483 1707
Size: 123 360 1224
Fragments arranged by size:
1224 360 123
XmaI C'CCGG_G
Cuts at: 0 896 1707
Size: 896 811
Enzymes that do cut:
AarI Acc65I AcuI AleI AlwNI ApaI AvaI BamHI
BanI BanII BbvCI BcgI BglI BglII BlpI Bme1580I
BmgBI BmrI BpmI Bpu10I BsaI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstXI
BstYI BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI Eco57MI EcoO109I Hin4I KpnI MscI MslI MspA1I
NaeI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI
PstI PvuII RsrII SacII SanDI SfcI SfiI SmaI
StuI StyI TaqII TatI TstI XcmI XmaI
Enzymes that do not cut:
AatII AccI AclI AfeI AflII AflIII AgeI AhdI
AlfI AloI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BciVI BclI BfrBI BmtI BplI
BpuEI BsaAI BsaBI BsaHI BsiWI BssHII BssSI BstBI
BstEII BstZ17I Bsu36I ClaI CspCI DraI DraIII EcoICRI
EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII
HincII HindIII HpaI KasI MfeI MluI MmeI NarI
NcoI NdeI NheI NotI NruI NsiI PacI PciI
PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI
SacI SalI SapI SbfI ScaI SexAI SfoI SgfI
SgrAI SmlI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmnI ZraI