Restriction map for
pF1KE0338
(Linear) (Six-base) MAPSORT of: KIEE0338.seq Check: 4925 from: 1 to: 1515
KIEE0338.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 341 581 940 1515
Size: 341 240 359 575
Fragments arranged by size:
575 359 341 240
AflII C'TTAA_G
Cuts at: 0 537 1515
Size: 537 978
AflIII A'CryG_T
Cuts at: 0 1035 1515
Size: 1035 480
AlwNI CAG_nnn'CTG
Cuts at: 0 937 1515
Size: 937 578
AvaI C'yCGr_G
Cuts at: 0 1304 1515
Size: 1304 211
BamHI G'GATC_C
Cuts at: 0 1126 1515
Size: 1126 389
BanI G'GyrC_C
Cuts at: 0 1207 1515
Size: 1207 308
BbsI GAAGACnn'nnnn_
Cuts at: 0 569 996 1515
Size: 569 427 519
Fragments arranged by size:
569 519 427
BciVI GTATCCnnnnn_n'
Cuts at: 0 342 1515
Size: 342 1173
BclI T'GATC_A
Cuts at: 0 1505 1515
Size: 1505 10
Bme1580I G_kGCm'C
Cuts at: 0 83 1515
Size: 83 1432
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 145 1515
Size: 78 67 1370
Fragments arranged by size:
1370 78 67
BsaI GGTCTCn'nnnn_
Cuts at: 0 1255 1485 1515
Size: 1255 230 30
Fragments arranged by size:
1255 230 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 595 1136 1515
Size: 520 75 541 379
Fragments arranged by size:
541 520 379 75
BseYI C'CCAG_C
Cuts at: 0 937 1515
Size: 937 578
BsiHKAI G_wGCw'C
Cuts at: 0 575 888 1199 1515
Size: 575 313 311 316
Fragments arranged by size:
575 316 313 311
BsmI GAATG_Cn'
Cuts at: 0 159 495 1515
Size: 159 336 1020
Fragments arranged by size:
1020 336 159
Bsp1286I G_dGCh'C
Cuts at: 0 83 575 888 1199 1515
Size: 83 492 313 311 316
Fragments arranged by size:
492 316 313 311 83
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 81 1459 1515
Size: 81 1378 56
Fragments arranged by size:
1378 81 56
BsrBI CCG'CTC
Cuts at: 0 962 1288 1515
Size: 962 326 227
Fragments arranged by size:
962 326 227
BsrDI GCAATG_nn'
Cuts at: 0 216 1061 1160 1515
Size: 216 845 99 355
Fragments arranged by size:
845 355 216 99
BstXI CCAn_nnnn'nTGG
Cuts at: 0 501 1183 1462 1515
Size: 501 682 279 53
Fragments arranged by size:
682 501 279 53
BstYI r'GATC_y
Cuts at: 0 1126 1515
Size: 1126 389
BtgI C'CryG_G
Cuts at: 0 1211 1515
Size: 1211 304
BtsI GCAGTG_nn'
Cuts at: 0 573 1515
Size: 573 942
ClaI AT'CG_AT
Cuts at: 0 240 1515
Size: 240 1275
EarI CTCTTCn'nnn_
Cuts at: 0 838 969 1400 1515
Size: 838 131 431 115
Fragments arranged by size:
838 431 131 115
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 78 145 341 581 940 1515
Size: 78 67 196 240 359 575
Fragments arranged by size:
575 359 240 196 78 67
EcoO109I rG'GnC_Cy
Cuts at: 0 659 1515
Size: 659 856
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 90 1515
Size: 90 1425
HincII GTy'rAC
Cuts at: 0 957 1515
Size: 957 558
MfeI C'AATT_G
Cuts at: 0 1496 1515
Size: 1496 19
MslI CAynn'nnrTG
Cuts at: 0 484 1515
Size: 484 1031
MspA1I CmG'CkG
Cuts at: 0 934 1515
Size: 934 581
NcoI C'CATG_G
Cuts at: 0 1211 1515
Size: 1211 304
NspI r_CATG'y
Cuts at: 0 90 1039 1084 1515
Size: 90 949 45 431
Fragments arranged by size:
949 431 90 45
PasI CC'CwG_GG
Cuts at: 0 663 1515
Size: 663 852
PciI A'CATG_T
Cuts at: 0 1035 1515
Size: 1035 480
PflMI CCAn_nnn'nTGG
Cuts at: 0 699 1515
Size: 699 816
PfoI T'CCnGG_A
Cuts at: 0 1334 1515
Size: 1334 181
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 13 45 1515
Size: 13 32 1470
Fragments arranged by size:
1470 32 13
PpuMI rG'GwC_Cy
Cuts at: 0 659 1515
Size: 659 856
PstI C_TGCA'G
Cuts at: 0 29 602 1515
Size: 29 573 913
Fragments arranged by size:
913 573 29
PvuII CAG'CTG
Cuts at: 0 934 1515
Size: 934 581
SanDI GG'GwC_CC
Cuts at: 0 659 1515
Size: 659 856
SapI GCTCTTCn'nnn_
Cuts at: 0 969 1515
Size: 969 546
SfcI C'TryA_G
Cuts at: 0 25 598 1221 1515
Size: 25 573 623 294
Fragments arranged by size:
623 573 294 25
SmlI C'TyrA_G
Cuts at: 0 537 1515
Size: 537 978
StuI AGG'CCT
Cuts at: 0 511 644 1515
Size: 511 133 871
Fragments arranged by size:
871 511 133
StyI C'CwwG_G
Cuts at: 0 512 998 1211 1515
Size: 512 486 213 304
Fragments arranged by size:
512 486 304 213
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1404 1515
Size: 1404 111
TatI w'GTAC_w
Cuts at: 0 1113 1515
Size: 1113 402
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1178 1210 1515
Size: 1178 32 305
Fragments arranged by size:
1178 305 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 271 454 816 1398 1515
Size: 271 183 362 582 117
Fragments arranged by size:
582 362 271 183 117
XmnI GAAnn'nnTTC
Cuts at: 0 73 894 1515
Size: 73 821 621
Fragments arranged by size:
821 621 73
Enzymes that do cut:
AcuI AflII AflIII AlwNI AvaI BamHI BanI BbsI
BciVI BclI Bme1580I BpmI BsaI BseRI BseYI BsiHKAI
BsmI Bsp1286I BspMI BsrBI BsrDI BstXI BstYI BtgI
BtsI ClaI EarI Eco57MI EcoO109I FalI HincII MfeI
MslI MspA1I NcoI NspI PasI PciI PflMI PfoI
PpiI PpuMI PstI PvuII SanDI SapI SfcI SmlI
StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AleI AlfI AloI ApaI ApaLI ApoI AscI AseI
AsiSI AvrII BaeI BanII BbeI BbvCI BcgI BfrBI
BglI BglII BlpI BmgBI BmrI BmtI BplI Bpu10I
BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsgI BsiEI
BsiWI BsmBI BspEI BspHI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstZ17I Bsu36I BtgZI CspCI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoRI
EcoRV FseI FspI FspAI HaeII Hin4I HindIII HpaI
KasI KpnI MluI MmeI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PmeI PmlI
PshAI PsiI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI SwaI
Tth111I XbaI XhoI XmaI ZraI