Restriction map for
pF1KE0330
(Linear) (Six-base) MAPSORT of: KIEE0330.seq Check: 9504 from: 1 to: 1824
KIEE0330.orf
With 182 enzymes: SgfI *
April 24, 2009 13:13 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 1093 1255 1335 1824
Size: 809 284 162 80 489
Fragments arranged by size:
809 489 284 162 80
AhdI GACnn_n'nnGTC
Cuts at: 0 1292 1824
Size: 1292 532
AlwNI CAG_nnn'CTG
Cuts at: 0 258 290 1824
Size: 258 32 1534
Fragments arranged by size:
1534 258 32
ApaI G_GGCC'C
Cuts at: 0 111 1824
Size: 111 1713
ApoI r'AATT_y
Cuts at: 0 574 1300 1824
Size: 574 726 524
Fragments arranged by size:
726 574 524
AvaI C'yCGr_G
Cuts at: 0 44 201 1824
Size: 44 157 1623
Fragments arranged by size:
1623 157 44
AvrII C'CTAG_G
Cuts at: 0 579 1824
Size: 579 1245
BamHI G'GATC_C
Cuts at: 0 25 1824
Size: 25 1799
BanI G'GyrC_C
Cuts at: 0 146 1518 1679 1824
Size: 146 1372 161 145
Fragments arranged by size:
1372 161 146 145
BanII G_rGCy'C
Cuts at: 0 111 224 449 787 1313 1397 1824
Size: 111 113 225 338 526 84 427
Fragments arranged by size:
526 427 338 225 113 111 84
BbeI G_GCGC'C
Cuts at: 0 150 1824
Size: 150 1674
BbsI GAAGACnn'nnnn_
Cuts at: 0 584 1824
Size: 584 1240
BbvCI CC'TCA_GC
Cuts at: 0 1184 1688 1824
Size: 1184 504 136
Fragments arranged by size:
1184 504 136
BglI GCCn_nnn'nGGC
Cuts at: 0 38 1060 1824
Size: 38 1022 764
Fragments arranged by size:
1022 764 38
BglII A'GATC_T
Cuts at: 0 585 1824
Size: 585 1239
BlpI GC'TnA_GC
Cuts at: 0 358 1642 1824
Size: 358 1284 182
Fragments arranged by size:
1284 358 182
Bme1580I G_kGCm'C
Cuts at: 0 111 1548 1824
Size: 111 1437 276
Fragments arranged by size:
1437 276 111
BmrI ACTGGGnnnn_n'
Cuts at: 0 1542 1824
Size: 1542 282
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 928 1327 1824
Size: 928 399 497
Fragments arranged by size:
928 497 399
Bpu10I CC'TnA_GC
Cuts at: 0 1184 1688 1824
Size: 1184 504 136
Fragments arranged by size:
1184 504 136
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 372 1049 1824
Size: 372 677 775
Fragments arranged by size:
775 677 372
BsaHI Gr'CG_yC
Cuts at: 0 147 236 1824
Size: 147 89 1588
Fragments arranged by size:
1588 147 89
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 1201 1528 1824
Size: 124 1077 327 296
Fragments arranged by size:
1077 327 296 124
BseYI C'CCAG_C
Cuts at: 0 1720 1824
Size: 1720 104
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1766 1824
Size: 1766 58
BsiEI CG_ry'CG
Cuts at: 0 102 1824
Size: 102 1722
BsiHKAI G_wGCw'C
Cuts at: 0 1313 1397 1643 1824
Size: 1313 84 246 181
Fragments arranged by size:
1313 246 181 84
BsmI GAATG_Cn'
Cuts at: 0 702 928 1202 1706 1824
Size: 702 226 274 504 118
Fragments arranged by size:
702 504 274 226 118
Bsp1286I G_dGCh'C
Cuts at: 0 111 224 449 787 1313 1397 1548 1643
Size: 111 113 225 338 526 84 151 95
Cuts at: 1643 1824
Size: 181
Fragments arranged by size:
526 338 225 181 151 113 111 95
84
BspHI T'CATG_A
Cuts at: 0 1248 1824
Size: 1248 576
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 153 898 1824
Size: 153 745 926
Fragments arranged by size:
926 745 153
BsrBI CCG'CTC
Cuts at: 0 49 200 489 1038 1824
Size: 49 151 289 549 786
Fragments arranged by size:
786 549 289 151 49
BsrFI r'CCGG_y
Cuts at: 0 37 97 1824
Size: 37 60 1727
Fragments arranged by size:
1727 60 37
BstAPI GCAn_nnn'nTGC
Cuts at: 0 258 1824
Size: 258 1566
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1126 1824
Size: 1126 698
BstYI r'GATC_y
Cuts at: 0 25 287 585 1824
Size: 25 262 298 1239
Fragments arranged by size:
1239 298 262 25
Bsu36I CC'TnA_GG
Cuts at: 0 1228 1750 1824
Size: 1228 522 74
Fragments arranged by size:
1228 522 74
BtgI C'CryG_G
Cuts at: 0 67 102 184 1049 1824
Size: 67 35 82 865 775
Fragments arranged by size:
865 775 82 67 35
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 729 1824
Size: 729 1095
BtsI GCAGTG_nn'
Cuts at: 0 1108 1150 1824
Size: 1108 42 674
Fragments arranged by size:
1108 674 42
DraIII CAC_nnn'GTG
Cuts at: 0 902 1137 1824
Size: 902 235 687
Fragments arranged by size:
902 687 235
EaeI y'GGCC_r
Cuts at: 0 35 99 1052 1824
Size: 35 64 953 772
Fragments arranged by size:
953 772 64 35
EagI C'GGCC_G
Cuts at: 0 99 1824
Size: 99 1725
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1567 1824
Size: 1567 257
EcoICRI GAG'CTC
Cuts at: 0 1311 1395 1824
Size: 1311 84 429
Fragments arranged by size:
1311 429 84
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 809 928 1093 1255 1327 1335 1824
Size: 809 119 165 162 72 8 489
Fragments arranged by size:
809 489 165 162 119 72 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 846 1687 1824
Size: 846 841 137
Fragments arranged by size:
846 841 137
EcoO109I rG'GnC_Cy
Cuts at: 0 107 108 165 483 851 887 1164 1824
Size: 107 1 57 318 368 36 277 660
Fragments arranged by size:
660 368 318 277 107 57 36 1
EcoRI G'AATT_C
Cuts at: 0 1300 1824
Size: 1300 524
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1396 1824
Size: 1396 428
HaeII r_GCGC'y
Cuts at: 0 150 1824
Size: 150 1674
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 184 216 279 311 436 468 1278 1310
Size: 184 32 63 32 125 32 810 32
Cuts at: 1310 1824
Size: 514
Fragments arranged by size:
810 514 184 125 63 32 32 32
32
HindIII A'AGCT_T
Cuts at: 0 605 1102 1824
Size: 605 497 722
Fragments arranged by size:
722 605 497
KasI G'GCGC_C
Cuts at: 0 146 1824
Size: 146 1678
MspA1I CmG'CkG
Cuts at: 0 69 104 186 1824
Size: 69 35 82 1638
Fragments arranged by size:
1638 82 69 35
NaeI GCC'GGC
Cuts at: 0 39 99 1824
Size: 39 60 1725
Fragments arranged by size:
1725 60 39
NarI GG'CG_CC
Cuts at: 0 147 1824
Size: 147 1677
NdeI CA'TA_TG
Cuts at: 0 1800 1824
Size: 1800 24
NgoMIV G'CCGG_C
Cuts at: 0 37 97 1824
Size: 37 60 1727
Fragments arranged by size:
1727 60 37
PflMI CCAn_nnn'nTGG
Cuts at: 0 951 1824
Size: 951 873
PfoI T'CCnGG_A
Cuts at: 0 1304 1824
Size: 1304 520
PpuMI rG'GwC_Cy
Cuts at: 0 165 483 851 887 1164 1824
Size: 165 318 368 36 277 660
Fragments arranged by size:
660 368 318 277 165 36
PsiI TTA'TAA
Cuts at: 0 1408 1824
Size: 1408 416
PspOMI G'GGCC_C
Cuts at: 0 107 1824
Size: 107 1717
PstI C_TGCA'G
Cuts at: 0 1197 1763 1824
Size: 1197 566 61
Fragments arranged by size:
1197 566 61
SacI G_AGCT'C
Cuts at: 0 1313 1397 1824
Size: 1313 84 427
Fragments arranged by size:
1313 427 84
SacII CC_GC'GG
Cuts at: 0 70 105 187 1824
Size: 70 35 82 1637
Fragments arranged by size:
1637 82 70 35
SanDI GG'GwC_CC
Cuts at: 0 1164 1824
Size: 1164 660
SfcI C'TryA_G
Cuts at: 0 291 1193 1759 1824
Size: 291 902 566 65
Fragments arranged by size:
902 566 291 65
SfoI GGC'GCC
Cuts at: 0 148 1824
Size: 148 1676
SmlI C'TyrA_G
Cuts at: 0 44 387 1064 1824
Size: 44 343 677 760
Fragments arranged by size:
760 677 343 44
SpeI A'CTAG_T
Cuts at: 0 55 1824
Size: 55 1769
StyI C'CwwG_G
Cuts at: 0 579 1824
Size: 579 1245
TatI w'GTAC_w
Cuts at: 0 678 1625 1824
Size: 678 947 199
Fragments arranged by size:
947 678 199
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1100 1121 1132 1153 1824
Size: 1100 21 11 21 671
Fragments arranged by size:
1100 671 21 21 11
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1056 1824
Size: 1056 768
XhoI C'TCGA_G
Cuts at: 0 44 1824
Size: 44 1780
XmnI GAAnn'nnTTC
Cuts at: 0 535 1376 1824
Size: 535 841 448
Fragments arranged by size:
841 535 448
Enzymes that do cut:
AcuI AhdI AlwNI ApaI ApoI AvaI AvrII BamHI
BanI BanII BbeI BbsI BbvCI BglI BglII BlpI
Bme1580I BmrI BpmI Bpu10I BpuEI BsaHI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BsrBI
BsrFI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EagI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI FalI HaeII Hin4I HindIII KasI MspA1I NaeI
NarI NdeI NgoMIV PflMI PfoI PpuMI PsiI PspOMI
PstI SacI SacII SanDI SfcI SfoI SmlI SpeI
StyI TatI TstI XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AleI AlfI AloI ApaLI AscI AseI AsiSI
BaeI BcgI BciVI BclI BfrBI BmgBI BmtI BplI
BsaI BsaAI BsaBI BsaWI BsaXI BsiWI BsmBI BspEI
BsrDI BsrGI BssHII BssSI BstBI BstEII BstZ17I ClaI
CspCI DraI DrdI EarI EcoRV FseI FspI FspAI
HincII HpaI KpnI MfeI MluI MmeI MscI MslI
NcoI NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PshAI PspXI PsrI PvuI
PvuII RsrII SalI SapI SbfI ScaI SexAI SfiI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI StuI
SwaI TaqII Tth111I XbaI XmaI ZraI