Restriction map for
pF1KE0291
(Linear) (Six-base) MAPSORT of: KIEE0291.seq Check: 1160 from: 1 to: 1221
KIEE0291.orf
With 182 enzymes: SgfI *
April 24, 2009 13:12 ..
AccI GT'mk_AC
Cuts at: 0 489 1221
Size: 489 732
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 685 1221
Size: 172 513 536
Fragments arranged by size:
536 513 172
AflIII A'CryG_T
Cuts at: 0 838 1221
Size: 838 383
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 413 1221
Size: 413 808
AlwNI CAG_nnn'CTG
Cuts at: 0 1109 1221
Size: 1109 112
AseI AT'TA_AT
Cuts at: 0 734 1221
Size: 734 487
BanI G'GyrC_C
Cuts at: 0 817 1221
Size: 817 404
BanII G_rGCy'C
Cuts at: 0 160 216 257 344 533 578 1221
Size: 160 56 41 87 189 45 643
Fragments arranged by size:
643 189 160 87 56 45 41
BbsI GAAGACnn'nnnn_
Cuts at: 0 979 1221
Size: 979 242
BclI T'GATC_A
Cuts at: 0 323 1134 1221
Size: 323 811 87
Fragments arranged by size:
811 323 87
BlpI GC'TnA_GC
Cuts at: 0 235 1221
Size: 235 986
BmgBI CAC'GTC
Cuts at: 0 839 1221
Size: 839 382
BmrI ACTGGGnnnn_n'
Cuts at: 0 338 434 1221
Size: 338 96 787
Fragments arranged by size:
787 338 96
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 358 743 1221
Size: 64 294 385 478
Fragments arranged by size:
478 385 294 64
BsaI GGTCTCn'nnnn_
Cuts at: 0 541 1221
Size: 541 680
BsaBI GATnn'nnATC
Cuts at: 0 325 1221
Size: 325 896
BsaHI Gr'CG_yC
Cuts at: 0 832 1221
Size: 832 389
BsaWI w'CCGG_w
Cuts at: 0 570 1221
Size: 570 651
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1109 1221
Size: 1109 112
BseYI C'CCAG_C
Cuts at: 0 577 1221
Size: 577 644
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 614 1221
Size: 162 452 607
Fragments arranged by size:
607 452 162
BsiHKAI G_wGCw'C
Cuts at: 0 22 533 1221
Size: 22 511 688
Fragments arranged by size:
688 511 22
BsmBI CGTCTCn'nnnn_
Cuts at: 0 189 1221
Size: 189 1032
Bsp1286I G_dGCh'C
Cuts at: 0 22 160 216 257 344 533 578 1221
Size: 22 138 56 41 87 189 45 643
Fragments arranged by size:
643 189 138 87 56 45 41 22
BspEI T'CCGG_A
Cuts at: 0 570 1221
Size: 570 651
BsrDI GCAATG_nn'
Cuts at: 0 633 778 1221
Size: 633 145 443
Fragments arranged by size:
633 443 145
BstAPI GCAn_nnn'nTGC
Cuts at: 0 896 1221
Size: 896 325
BstXI CCAn_nnnn'nTGG
Cuts at: 0 721 1221
Size: 721 500
BstYI r'GATC_y
Cuts at: 0 39 394 1221
Size: 39 355 827
Fragments arranged by size:
827 355 39
BtgI C'CryG_G
Cuts at: 0 248 1221
Size: 248 973
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 87 1221
Size: 87 1134
BtsI GCAGTG_nn'
Cuts at: 0 95 602 1221
Size: 95 507 619
Fragments arranged by size:
619 507 95
DraIII CAC_nnn'GTG
Cuts at: 0 336 1221
Size: 336 885
EaeI y'GGCC_r
Cuts at: 0 1184 1221
Size: 1184 37
EarI CTCTTCn'nnn_
Cuts at: 0 652 1221
Size: 652 569
EcoICRI GAG'CTC
Cuts at: 0 531 1221
Size: 531 690
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 64 172 358 685 743 1221
Size: 64 108 186 327 58 478
Fragments arranged by size:
478 327 186 108 64 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 285 1221
Size: 285 936
EcoO109I rG'GnC_Cy
Cuts at: 0 130 1221
Size: 130 1091
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 820 1221
Size: 820 401
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 356 388 693 725 828 860 1221
Size: 356 32 305 32 103 32 361
Fragments arranged by size:
361 356 305 103 32 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1221
Size: 780 441
MscI TGG'CCA
Cuts at: 0 1186 1221
Size: 1186 35
MslI CAynn'nnrTG
Cuts at: 0 229 1221
Size: 229 992
MspA1I CmG'CkG
Cuts at: 0 61 1128 1221
Size: 61 1067 93
Fragments arranged by size:
1067 93 61
NcoI C'CATG_G
Cuts at: 0 248 1221
Size: 248 973
PasI CC'CwG_GG
Cuts at: 0 189 1221
Size: 189 1032
PflMI CCAn_nnn'nTGG
Cuts at: 0 336 420 1221
Size: 336 84 801
Fragments arranged by size:
801 336 84
PfoI T'CCnGG_A
Cuts at: 0 512 1221
Size: 512 709
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 298 330 1221
Size: 298 32 891
Fragments arranged by size:
891 298 32
SacI G_AGCT'C
Cuts at: 0 533 1221
Size: 533 688
SapI GCTCTTCn'nnn_
Cuts at: 0 652 1221
Size: 652 569
SfcI C'TryA_G
Cuts at: 0 918 1214 1221
Size: 918 296 7
Fragments arranged by size:
918 296 7
SspI AAT'ATT
Cuts at: 0 738 1221
Size: 738 483
StyI C'CwwG_G
Cuts at: 0 248 1221
Size: 248 973
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 523 1161 1221
Size: 523 638 60
Fragments arranged by size:
638 523 60
TatI w'GTAC_w
Cuts at: 0 841 1221
Size: 841 380
Enzymes that do cut:
AccI AcuI AflIII AlfI AlwNI AseI BanI BanII
BbsI BclI BlpI BmgBI BmrI BpmI BsaI BsaBI
BsaHI BsaWI BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I
BspEI BsrDI BstAPI BstXI BstYI BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
Hin4I MmeI MscI MslI MspA1I NcoI PasI PflMI
PfoI PpiI SacI SapI SfcI SspI StyI TaqII
TatI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AleI AloI ApaI ApaLI ApoI AscI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII Bme1580I BmtI BplI Bpu10I BpuEI BsaAI
BsaXI BsiEI BsiWI BsmI BspHI BspMI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I ClaI
CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI
FspI FspAI HaeII HincII HindIII HpaI KasI KpnI
MfeI MluI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpuMI
PshAI PsiI PspOMI PspXI PsrI PstI PvuI PvuII
RsrII SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StuI SwaI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI